Transitional cell cytokeratins as a second parameter in flow cytometry of bladder cancer
Edwards, B. F. ; Rossitto, P. V. ; Baker, W. C. ; Cardiff, R. D. ; Strand, M. ; Deitch, A. D. ; deVere White, R.
Springer
Published 1987
Springer
Published 1987
| ISSN: |
1433-8726
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| Source: |
Springer Online Journal Archives 1860-2000
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| Topics: |
Medicine
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| Notes: |
Summary Since the detection of abnormal DNA content in the cells of transitional cell carcinoma can be obscured by the presence in the population of diploid stromal and inflammatory cells, it would be desirable to have a second flow cytometric parameter to complement DNA analysis. The specific identification of carcinoma cells using monoclonal antibodies directed against epithelial intermediate filament proteins (cytokeratins) would provide a method with which to identify the transitional cells in solid tumors, voided urines or bladder washings. As a preliminary study we have tested cell-free urine samples using a double antibody radiometric assay (IRMAK) for cytokeratins released from transitional cells. The additional information from cytokeratin analyses increased the specificity of disease detection.
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| Type of Medium: |
Electronic Resource
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| URL: |