Large-scale cultivation of Solanum chrysotrichum cells: Production of the antifungal saponin SC-1 in 10-l airlift bioreactors
Villarreal, Ma. L. ; Arias, C. ; Vega, J. ; Feria-Velasco, A. ; Ramírez, O. T. ; Nicasio, P. ; Rojas, G. ; Quintero, R.
Springer
Published 1997
Springer
Published 1997
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1432-203X
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| Keywords: |
Key wordsSolanum chrysotrichum ; Cell suspension culture ; Spirostanol saponins ; Antimycotic ; Large-scale culture
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| Source: |
Springer Online Journal Archives 1860-2000
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| Topics: |
Biology
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| Notes: |
Abstract Cells of two different cell lines: ccvx (cotyledon derived) and ccvz (hypocotyl derived) of Solanum chrysotrichum were cultivated in 10-l airlift bioreactors for the production of the human antimycotic compound SC-1. When using 3 g l–1 dry weight inoculum in a batch culture, higher levels of biomass were achieved with the ccvx cell line (14.6 g l–1) than with ccvz (7.7 g l–1), resulting in 23 and 12 mg g–1 of SC-1 after 17 days in culture for ccvx and ccvz, respectively. The maximum productivity of SC-1 in bioreactors was 0.025 g l–1 day–1 after 9 days in culture. When using a draw-fill mode, the productivity increased by 60% to a value of 0.041 g l–1 day–1, 4 days after 50% of the cell suspension was removed and replaced with fresh medium. This latter bioreactor system is a feasible alternative for the production of the antimycotic metabolite of S. chrysotrichum on a large scale.
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Electronic Resource
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