Analysis of intra-specific somatic hybrids of potato (Solanum tuberosum) using simple sequence repeats
| ISSN: |
1432-203X
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|---|---|
| Keywords: |
Key words: potato, simple sequence repeats, somatic hybrids
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| Source: |
Springer Online Journal Archives 1860-2000
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| Topics: |
Biology
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| Notes: |
Abstract. We have utilised simple sequence repeat (SSR) polymorphism to analyse two sets of potential intra-specific hybrids of potato. Two primer pairs were used and both showed that one set of fusion products could not be true heterokaryons. In the other set, one of the primer pairs showed that unique bands in each of the parents were present in all of the hybrids, unambiguously demonstrating hybridity. This simple and robust, high-resolution assay can be used at the callus level and is amenable to automation, making it possible to reduce greatly the time required to screen a large number of potential somatic hybrids.
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| Type of Medium: |
Electronic Resource
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| URL: |
| _version_ | 1798295906280275968 |
|---|---|
| autor | Provan, J. Kumar, A. Shepherd, L. Powell, W. Waugh, R. |
| autorsonst | Provan, J. Kumar, A. Shepherd, L. Powell, W. Waugh, R. |
| book_url | http://dx.doi.org/10.1007/s002990050205 |
| datenlieferant | nat_lic_papers |
| hauptsatz | hsatz_simple |
| identnr | NLM203160118 |
| iqvoc_descriptor_title | iqvoc_00000708:Analysis |
| issn | 1432-203X |
| journal_name | Plant cell reports |
| materialart | 1 |
| notes | Abstract. We have utilised simple sequence repeat (SSR) polymorphism to analyse two sets of potential intra-specific hybrids of potato. Two primer pairs were used and both showed that one set of fusion products could not be true heterokaryons. In the other set, one of the primer pairs showed that unique bands in each of the parents were present in all of the hybrids, unambiguously demonstrating hybridity. This simple and robust, high-resolution assay can be used at the callus level and is amenable to automation, making it possible to reduce greatly the time required to screen a large number of potential somatic hybrids. |
| package_name | Springer |
| publikationsjahr_anzeige | 1996 |
| publikationsjahr_facette | 1996 |
| publikationsjahr_intervall | 8004:1995-1999 |
| publikationsjahr_sort | 1996 |
| publisher | Springer |
| reference | 16 (1996), S. 196-199 |
| schlagwort | Key words: potato, simple sequence repeats, somatic hybrids |
| search_space | articles |
| shingle_author_1 | Provan, J. Kumar, A. Shepherd, L. Powell, W. Waugh, R. |
| shingle_author_2 | Provan, J. Kumar, A. Shepherd, L. Powell, W. Waugh, R. |
| shingle_author_3 | Provan, J. Kumar, A. Shepherd, L. Powell, W. Waugh, R. |
| shingle_author_4 | Provan, J. Kumar, A. Shepherd, L. Powell, W. Waugh, R. |
| shingle_catch_all_1 | Provan, J. Kumar, A. Shepherd, L. Powell, W. Waugh, R. Analysis of intra-specific somatic hybrids of potato (Solanum tuberosum) using simple sequence repeats Key words: potato, simple sequence repeats, somatic hybrids Key words: potato, simple sequence repeats, somatic hybrids Abstract. We have utilised simple sequence repeat (SSR) polymorphism to analyse two sets of potential intra-specific hybrids of potato. Two primer pairs were used and both showed that one set of fusion products could not be true heterokaryons. In the other set, one of the primer pairs showed that unique bands in each of the parents were present in all of the hybrids, unambiguously demonstrating hybridity. This simple and robust, high-resolution assay can be used at the callus level and is amenable to automation, making it possible to reduce greatly the time required to screen a large number of potential somatic hybrids. 1432-203X 1432203X Springer |
| shingle_catch_all_2 | Provan, J. Kumar, A. Shepherd, L. Powell, W. Waugh, R. Analysis of intra-specific somatic hybrids of potato (Solanum tuberosum) using simple sequence repeats Key words: potato, simple sequence repeats, somatic hybrids Key words: potato, simple sequence repeats, somatic hybrids Abstract. We have utilised simple sequence repeat (SSR) polymorphism to analyse two sets of potential intra-specific hybrids of potato. Two primer pairs were used and both showed that one set of fusion products could not be true heterokaryons. In the other set, one of the primer pairs showed that unique bands in each of the parents were present in all of the hybrids, unambiguously demonstrating hybridity. This simple and robust, high-resolution assay can be used at the callus level and is amenable to automation, making it possible to reduce greatly the time required to screen a large number of potential somatic hybrids. 1432-203X 1432203X Springer |
| shingle_catch_all_3 | Provan, J. Kumar, A. Shepherd, L. Powell, W. Waugh, R. Analysis of intra-specific somatic hybrids of potato (Solanum tuberosum) using simple sequence repeats Key words: potato, simple sequence repeats, somatic hybrids Key words: potato, simple sequence repeats, somatic hybrids Abstract. We have utilised simple sequence repeat (SSR) polymorphism to analyse two sets of potential intra-specific hybrids of potato. Two primer pairs were used and both showed that one set of fusion products could not be true heterokaryons. In the other set, one of the primer pairs showed that unique bands in each of the parents were present in all of the hybrids, unambiguously demonstrating hybridity. This simple and robust, high-resolution assay can be used at the callus level and is amenable to automation, making it possible to reduce greatly the time required to screen a large number of potential somatic hybrids. 1432-203X 1432203X Springer |
| shingle_catch_all_4 | Provan, J. Kumar, A. Shepherd, L. Powell, W. Waugh, R. Analysis of intra-specific somatic hybrids of potato (Solanum tuberosum) using simple sequence repeats Key words: potato, simple sequence repeats, somatic hybrids Key words: potato, simple sequence repeats, somatic hybrids Abstract. We have utilised simple sequence repeat (SSR) polymorphism to analyse two sets of potential intra-specific hybrids of potato. Two primer pairs were used and both showed that one set of fusion products could not be true heterokaryons. In the other set, one of the primer pairs showed that unique bands in each of the parents were present in all of the hybrids, unambiguously demonstrating hybridity. This simple and robust, high-resolution assay can be used at the callus level and is amenable to automation, making it possible to reduce greatly the time required to screen a large number of potential somatic hybrids. 1432-203X 1432203X Springer |
| shingle_title_1 | Analysis of intra-specific somatic hybrids of potato (Solanum tuberosum) using simple sequence repeats |
| shingle_title_2 | Analysis of intra-specific somatic hybrids of potato (Solanum tuberosum) using simple sequence repeats |
| shingle_title_3 | Analysis of intra-specific somatic hybrids of potato (Solanum tuberosum) using simple sequence repeats |
| shingle_title_4 | Analysis of intra-specific somatic hybrids of potato (Solanum tuberosum) using simple sequence repeats |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Springer Online Journal Archives 1860-2000 |
| timestamp | 2024-05-06T09:43:38.847Z |
| titel | Analysis of intra-specific somatic hybrids of potato (Solanum tuberosum) using simple sequence repeats |
| titel_suche | Analysis of intra-specific somatic hybrids of potato (Solanum tuberosum) using simple sequence repeats |
| topic | W |
| uid | nat_lic_papers_NLM203160118 |