Separation and characterization of complement-fixing immune complexes in juvenile rheumatoid arthritis patients
| ISSN: |
1437-160X
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|---|---|
| Keywords: |
Juvenile rheumatoid arthritis ; Immune complexes ; IgM and IgG rheumatoid factors
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| Source: |
Springer Online Journal Archives 1860-2000
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| Topics: |
Medicine
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| Notes: |
Summary Immune complexes (IC) in sera from patients with juvenile rheumatoid arthritis (JRA) were isolated by the use of immunoabsorbent columns. Sera from 14 JRA patients (four seropositive for 19S IgM RF and 10 seronegative, but nine having hidden 19S IgM RF) were analyzed by the anti-human Clq (αHClq) and anti-human C3 (αHC3) columns. The columns were sequentially eluted with veronal buffer, 0.02 M EDTA, 0.5 M NaCl, and 1 M propionic acid. By the αHClq column, IgM RF were detected in at least one of the separated IC fractions of 13 of 14 patients and IgG RF in three patients. By the αHC3 column, only five patients demonstrated IgM RF and only one IgG RF in the eluted fractions. On sucrose density gradient analysis (SDGA), all IC were demonstrated in the peaks ≧19S. 19S IgM RF were demonstrated by ELISA in all 14 patients, but IgG RF in only three. These studies demonstrate that complement-fixing 19S IgM RF, IgG, and IgG RF containing IC can be detected in the serum of JRA patients.
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| Type of Medium: |
Electronic Resource
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| URL: |
| _version_ | 1798296277931261954 |
|---|---|
| autor | Moore, T. L. Dorner, R. W. |
| autorsonst | Moore, T. L. Dorner, R. W. |
| book_url | http://dx.doi.org/10.1007/BF00541503 |
| datenlieferant | nat_lic_papers |
| hauptsatz | hsatz_simple |
| identnr | NLM203145011 |
| issn | 1437-160X |
| journal_name | Rheumatology international |
| materialart | 1 |
| notes | Summary Immune complexes (IC) in sera from patients with juvenile rheumatoid arthritis (JRA) were isolated by the use of immunoabsorbent columns. Sera from 14 JRA patients (four seropositive for 19S IgM RF and 10 seronegative, but nine having hidden 19S IgM RF) were analyzed by the anti-human Clq (αHClq) and anti-human C3 (αHC3) columns. The columns were sequentially eluted with veronal buffer, 0.02 M EDTA, 0.5 M NaCl, and 1 M propionic acid. By the αHClq column, IgM RF were detected in at least one of the separated IC fractions of 13 of 14 patients and IgG RF in three patients. By the αHC3 column, only five patients demonstrated IgM RF and only one IgG RF in the eluted fractions. On sucrose density gradient analysis (SDGA), all IC were demonstrated in the peaks ≧19S. 19S IgM RF were demonstrated by ELISA in all 14 patients, but IgG RF in only three. These studies demonstrate that complement-fixing 19S IgM RF, IgG, and IgG RF containing IC can be detected in the serum of JRA patients. |
| package_name | Springer |
| publikationsjahr_anzeige | 1986 |
| publikationsjahr_facette | 1986 |
| publikationsjahr_intervall | 8014:1985-1989 |
| publikationsjahr_sort | 1986 |
| publisher | Springer |
| reference | 6 (1986), S. 49-52 |
| schlagwort | Juvenile rheumatoid arthritis Immune complexes IgM and IgG rheumatoid factors |
| search_space | articles |
| shingle_author_1 | Moore, T. L. Dorner, R. W. |
| shingle_author_2 | Moore, T. L. Dorner, R. W. |
| shingle_author_3 | Moore, T. L. Dorner, R. W. |
| shingle_author_4 | Moore, T. L. Dorner, R. W. |
| shingle_catch_all_1 | Moore, T. L. Dorner, R. W. Separation and characterization of complement-fixing immune complexes in juvenile rheumatoid arthritis patients Juvenile rheumatoid arthritis Immune complexes IgM and IgG rheumatoid factors Juvenile rheumatoid arthritis Immune complexes IgM and IgG rheumatoid factors Summary Immune complexes (IC) in sera from patients with juvenile rheumatoid arthritis (JRA) were isolated by the use of immunoabsorbent columns. Sera from 14 JRA patients (four seropositive for 19S IgM RF and 10 seronegative, but nine having hidden 19S IgM RF) were analyzed by the anti-human Clq (αHClq) and anti-human C3 (αHC3) columns. The columns were sequentially eluted with veronal buffer, 0.02 M EDTA, 0.5 M NaCl, and 1 M propionic acid. By the αHClq column, IgM RF were detected in at least one of the separated IC fractions of 13 of 14 patients and IgG RF in three patients. By the αHC3 column, only five patients demonstrated IgM RF and only one IgG RF in the eluted fractions. On sucrose density gradient analysis (SDGA), all IC were demonstrated in the peaks ≧19S. 19S IgM RF were demonstrated by ELISA in all 14 patients, but IgG RF in only three. These studies demonstrate that complement-fixing 19S IgM RF, IgG, and IgG RF containing IC can be detected in the serum of JRA patients. 1437-160X 1437160X Springer |
| shingle_catch_all_2 | Moore, T. L. Dorner, R. W. Separation and characterization of complement-fixing immune complexes in juvenile rheumatoid arthritis patients Juvenile rheumatoid arthritis Immune complexes IgM and IgG rheumatoid factors Juvenile rheumatoid arthritis Immune complexes IgM and IgG rheumatoid factors Summary Immune complexes (IC) in sera from patients with juvenile rheumatoid arthritis (JRA) were isolated by the use of immunoabsorbent columns. Sera from 14 JRA patients (four seropositive for 19S IgM RF and 10 seronegative, but nine having hidden 19S IgM RF) were analyzed by the anti-human Clq (αHClq) and anti-human C3 (αHC3) columns. The columns were sequentially eluted with veronal buffer, 0.02 M EDTA, 0.5 M NaCl, and 1 M propionic acid. By the αHClq column, IgM RF were detected in at least one of the separated IC fractions of 13 of 14 patients and IgG RF in three patients. By the αHC3 column, only five patients demonstrated IgM RF and only one IgG RF in the eluted fractions. On sucrose density gradient analysis (SDGA), all IC were demonstrated in the peaks ≧19S. 19S IgM RF were demonstrated by ELISA in all 14 patients, but IgG RF in only three. These studies demonstrate that complement-fixing 19S IgM RF, IgG, and IgG RF containing IC can be detected in the serum of JRA patients. 1437-160X 1437160X Springer |
| shingle_catch_all_3 | Moore, T. L. Dorner, R. W. Separation and characterization of complement-fixing immune complexes in juvenile rheumatoid arthritis patients Juvenile rheumatoid arthritis Immune complexes IgM and IgG rheumatoid factors Juvenile rheumatoid arthritis Immune complexes IgM and IgG rheumatoid factors Summary Immune complexes (IC) in sera from patients with juvenile rheumatoid arthritis (JRA) were isolated by the use of immunoabsorbent columns. Sera from 14 JRA patients (four seropositive for 19S IgM RF and 10 seronegative, but nine having hidden 19S IgM RF) were analyzed by the anti-human Clq (αHClq) and anti-human C3 (αHC3) columns. The columns were sequentially eluted with veronal buffer, 0.02 M EDTA, 0.5 M NaCl, and 1 M propionic acid. By the αHClq column, IgM RF were detected in at least one of the separated IC fractions of 13 of 14 patients and IgG RF in three patients. By the αHC3 column, only five patients demonstrated IgM RF and only one IgG RF in the eluted fractions. On sucrose density gradient analysis (SDGA), all IC were demonstrated in the peaks ≧19S. 19S IgM RF were demonstrated by ELISA in all 14 patients, but IgG RF in only three. These studies demonstrate that complement-fixing 19S IgM RF, IgG, and IgG RF containing IC can be detected in the serum of JRA patients. 1437-160X 1437160X Springer |
| shingle_catch_all_4 | Moore, T. L. Dorner, R. W. Separation and characterization of complement-fixing immune complexes in juvenile rheumatoid arthritis patients Juvenile rheumatoid arthritis Immune complexes IgM and IgG rheumatoid factors Juvenile rheumatoid arthritis Immune complexes IgM and IgG rheumatoid factors Summary Immune complexes (IC) in sera from patients with juvenile rheumatoid arthritis (JRA) were isolated by the use of immunoabsorbent columns. Sera from 14 JRA patients (four seropositive for 19S IgM RF and 10 seronegative, but nine having hidden 19S IgM RF) were analyzed by the anti-human Clq (αHClq) and anti-human C3 (αHC3) columns. The columns were sequentially eluted with veronal buffer, 0.02 M EDTA, 0.5 M NaCl, and 1 M propionic acid. By the αHClq column, IgM RF were detected in at least one of the separated IC fractions of 13 of 14 patients and IgG RF in three patients. By the αHC3 column, only five patients demonstrated IgM RF and only one IgG RF in the eluted fractions. On sucrose density gradient analysis (SDGA), all IC were demonstrated in the peaks ≧19S. 19S IgM RF were demonstrated by ELISA in all 14 patients, but IgG RF in only three. These studies demonstrate that complement-fixing 19S IgM RF, IgG, and IgG RF containing IC can be detected in the serum of JRA patients. 1437-160X 1437160X Springer |
| shingle_title_1 | Separation and characterization of complement-fixing immune complexes in juvenile rheumatoid arthritis patients |
| shingle_title_2 | Separation and characterization of complement-fixing immune complexes in juvenile rheumatoid arthritis patients |
| shingle_title_3 | Separation and characterization of complement-fixing immune complexes in juvenile rheumatoid arthritis patients |
| shingle_title_4 | Separation and characterization of complement-fixing immune complexes in juvenile rheumatoid arthritis patients |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Springer Online Journal Archives 1860-2000 |
| timestamp | 2024-05-06T09:49:32.445Z |
| titel | Separation and characterization of complement-fixing immune complexes in juvenile rheumatoid arthritis patients |
| titel_suche | Separation and characterization of complement-fixing immune complexes in juvenile rheumatoid arthritis patients |
| topic | WW-YZ |
| uid | nat_lic_papers_NLM203145011 |