1H NMR study of the interaction of bacteriophage λ Cro protein with the O R 3 operator
Hahn, K. D. ; Buck, F. ; Rüterjans, H. ; Chernov, B. K. ; Skryabin, K. G. ; Kirpichnikov, M. P.
Springer
Published 1985
Springer
Published 1985
| ISSN: |
1432-1017
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|---|---|
| Keywords: |
O R 3 operator ; nuclear magnetic resonance ; assignment of proton resonances ; two-dimensional nuclear Overhauser effect spectroscopy
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| Source: |
Springer Online Journal Archives 1860-2000
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| Topics: |
Biology
Physics
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| Notes: |
Abstract The 17 base pair operator O R 3 oligonucleotide, which is the preferential binding site for the Cro repressor of phage λ, was studied by two-dimensional NMR spectroscopy. A sequential assignment procedure based on two-dimensional Nuclear Overhauser Effect (NOESY) and scalar coupling correlated (COSY) NMR spectroscopy, together with the knowledge of the oligodesoxynucleotide sequence, made it possible to assign the non-exhangeable base protons and the H1′ and the H2′-H2″ sugar protons of the O R 3 operator DNA. The pattern of the observed NOE connectivities is consistent with a right-handed helical DNA structure. The base and sugar proton assignments provide the necessary information for further studies of the O R 3 operator — Cro repressor interaction.
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| Type of Medium: |
Electronic Resource
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| URL: |