Microdetermination of citric acid in nervous tissue

Naruse, H. ; Cheng, S. -C. ; Waelsch, H.
Springer
Published 1966
ISSN:
1432-1106
Keywords:
Citric acid ; Micro assay ; Peripheral nerve ; CNS
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary The separation on a Dowex-1-acetate column of glutamic, aspartic, lactic, succinic, and malic acids was described. The “citric acid” (citric, cisaconitic and isocitric acids), fumaric, and oxaloacetic acids were separated. A new method for microdetermination of citric acid was described also. TPNH in the presence of purified aconitase, and isocitric dehydrogenase, is measured fluorometrically. The method permits the determination of 1 mμmole citric acid. The citric acid content of peripheral nerve and brain was measured by this method.
Type of Medium:
Electronic Resource
URL: