Fractionation of polychlorinated dibenzo-p-dioxins, polychlorinated dibenzofurans and planar polychlorinated biphenyls by high performance liquid chromatography on a pyrenyl-silica column
Martínez-Cored, M. ; Pujadas, E. ; Díaz-Ferrero, J. ; Coll, M. ; Martí, R. ; Broto-Puig, F. ; Comellas, L. ; Rodríguez-Larena, M. C.
Springer
Published 1999
Springer
Published 1999
| ISSN: |
1432-1130
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| Source: |
Springer Online Journal Archives 1860-2000
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| Topics: |
Chemistry and Pharmacology
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| Notes: |
Abstract Analytical procedures for the determination of polychlorinated dibenzo-p-dioxins (PCDD), polychlorinated dibenzofurans (PCDF) and non-ortho polychlorinated biphenyls (PCB) require a fractionation step to separate PCDD/F from planar PCB and the bulk of PCB. An HPLC method which achieves the separation of the bulk of PCB (0–6 mL of hexane), mono-ortho PCB (6–8 mL of hexane), non-ortho PCB (8–15 mL of hexane) and PCDD/F (15–50 mL of toluene) on a PYE column (2-(1-pyrenyl) ethyldimethylsilylated silica gel) in a single step without the use of backflush as other authors proposed was developed. The method shows a good accuracy and precision and it is linear in the range studied, e.g from 5.8 to 2420 pg injected in HPLC for TCDD/F, from 28.8 to 12100 pg for PeCDD/F, HxCDD/F, HpCDD/F and from 57.6 to 24200 pg for OCDD/F. It has been successfully applied to the analysis of technical mixtures of PCB (Aroclors), a pine wood sample and several water samples of different origins.
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| Type of Medium: |
Electronic Resource
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| URL: |