High production of D-β-hydroxyisobutyric acid from methacrylic acid by Candida rugosa and its mutant
Lee, I. Y. ; Kim, C. H. ; Yeon, B. K. ; Hong, W. K. ; Choi, E. S. ; Rhee, S. K. ; Park, Y. H. ; Sung, D. H. ; Baek, W. H.
Springer
Published 1997
Springer
Published 1997
| ISSN: |
0178-515X
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| Source: |
Springer Online Journal Archives 1860-2000
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| Topics: |
Process Engineering, Biotechnology, Nutrition Technology
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| Notes: |
Abstract Production of D-β-hydroxyisobutyric acid (D-HIBA) from methacrylic acid (MA) was investigated using Candida rugosa IFO 0750 and its mutant. Cell growth decreased as the MA concentration increased and was inhibited at D-HIBA concentrations higher than 30 g/l. Optimal MA concentration for D-HIBA production was in the range of 10–20 g/l. It was also noted that cell growth and D-HIBA production were inhibited by higher concen-trations of Na+, K+, and NH4 +, which were required for pH control during cultivation. With a suitably designed feeding mode of MA, the parent strain produced 65 g/l of D-HIBA after 120 h of fed-batch cultivation, but molar conversion yield of D-HIBA was less than 40%. The mutant, unable to assimilate propionic acid, produced as high as 70 g/l of D-HIBA in the same culture period with a molar conversion yield of more than 70%.
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| Type of Medium: |
Electronic Resource
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| URL: |