The interferon-Á gene as a positional and functional candidate gene for inflammatory bowel disease

ISSN:
1432-1262
Keywords:
Key words Pro-inflammatory cytokines ; Crohn's disease ; Ulcerative colitis ; Susceptibility
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Epidemiological and genome-wide linkage analyses have provided firm evidence for a genetic component in the pathogenesis of inflammatory bowel disease. The linkage regions on chromosomes 12 and 16 have been replicated in several independent samples. These represent the best positional evidence in the search for inflammatory bowel disease susceptibility genes. While systematic association and physical mapping studies in these regions are under way, the direct analysis of immunologically relevant genes as positional and functional candidates may provide a shortcut in this process. The interferon-γ gene resides in the chromosome 12 linkage region near the marker D12S83. Interferon-γ is an important proinflammatory cytokine in the interleukin-12 cascade and has been implicated in the pathogenesis of mucosal inflammation. We tested this gene for evidence of linkage and association in 133 German multiplex families and 506 single patients with their parents. An intragenic, highly informative CA-repeat marker in intron 1 of the gene was typed using fluorescence-labeled polymerase chain reaction and analysis on an automated sequencer. In the nonparametric linkage analysis using GENEHUNTER, a nonsignificant maximum LOD score of 0.67 was obtained. The transmission disequilibrium test for association was negative (P≥0.22) for Crohn's disease, ulcerative colitis, and the combined inflammatory bowel disease phenotype. In summary, the findings make interferon-γ a very unlikely candidate for the major susceptibility gene in the chromosome 12 linkage interval. Future efforts can concentrate on other transcripts in the region.
Type of Medium:
Electronic Resource
URL:
_version_ 1798295644123693059
autor Hampe, J.
Hermann, B.
Bridger, S.
MacPherson, A. J. S.
Mathew, C. G.
Schreiber, S.
autorsonst Hampe, J.
Hermann, B.
Bridger, S.
MacPherson, A. J. S.
Mathew, C. G.
Schreiber, S.
book_url http://dx.doi.org/10.1007/s003840050173
datenlieferant nat_lic_papers
hauptsatz hsatz_simple
identnr NLM200835661
issn 1432-1262
journal_name International journal of colorectal disease
materialart 1
notes Abstract Epidemiological and genome-wide linkage analyses have provided firm evidence for a genetic component in the pathogenesis of inflammatory bowel disease. The linkage regions on chromosomes 12 and 16 have been replicated in several independent samples. These represent the best positional evidence in the search for inflammatory bowel disease susceptibility genes. While systematic association and physical mapping studies in these regions are under way, the direct analysis of immunologically relevant genes as positional and functional candidates may provide a shortcut in this process. The interferon-γ gene resides in the chromosome 12 linkage region near the marker D12S83. Interferon-γ is an important proinflammatory cytokine in the interleukin-12 cascade and has been implicated in the pathogenesis of mucosal inflammation. We tested this gene for evidence of linkage and association in 133 German multiplex families and 506 single patients with their parents. An intragenic, highly informative CA-repeat marker in intron 1 of the gene was typed using fluorescence-labeled polymerase chain reaction and analysis on an automated sequencer. In the nonparametric linkage analysis using GENEHUNTER, a nonsignificant maximum LOD score of 0.67 was obtained. The transmission disequilibrium test for association was negative (P≥0.22) for Crohn's disease, ulcerative colitis, and the combined inflammatory bowel disease phenotype. In summary, the findings make interferon-γ a very unlikely candidate for the major susceptibility gene in the chromosome 12 linkage interval. Future efforts can concentrate on other transcripts in the region.
package_name Springer
publikationsjahr_anzeige 1998
publikationsjahr_facette 1998
publikationsjahr_intervall 8004:1995-1999
publikationsjahr_sort 1998
publisher Springer
reference 13 (1998), S. 260-263
schlagwort Key words Pro-inflammatory cytokines
Crohn's disease
Ulcerative colitis
Susceptibility
search_space articles
shingle_author_1 Hampe, J.
Hermann, B.
Bridger, S.
MacPherson, A. J. S.
Mathew, C. G.
Schreiber, S.
shingle_author_2 Hampe, J.
Hermann, B.
Bridger, S.
MacPherson, A. J. S.
Mathew, C. G.
Schreiber, S.
shingle_author_3 Hampe, J.
Hermann, B.
Bridger, S.
MacPherson, A. J. S.
Mathew, C. G.
Schreiber, S.
shingle_author_4 Hampe, J.
Hermann, B.
Bridger, S.
MacPherson, A. J. S.
Mathew, C. G.
Schreiber, S.
shingle_catch_all_1 Hampe, J.
Hermann, B.
Bridger, S.
MacPherson, A. J. S.
Mathew, C. G.
Schreiber, S.
The interferon-Á gene as a positional and functional candidate gene for inflammatory bowel disease
Key words Pro-inflammatory cytokines
Crohn's disease
Ulcerative colitis
Susceptibility
Key words Pro-inflammatory cytokines
Crohn's disease
Ulcerative colitis
Susceptibility
Abstract Epidemiological and genome-wide linkage analyses have provided firm evidence for a genetic component in the pathogenesis of inflammatory bowel disease. The linkage regions on chromosomes 12 and 16 have been replicated in several independent samples. These represent the best positional evidence in the search for inflammatory bowel disease susceptibility genes. While systematic association and physical mapping studies in these regions are under way, the direct analysis of immunologically relevant genes as positional and functional candidates may provide a shortcut in this process. The interferon-γ gene resides in the chromosome 12 linkage region near the marker D12S83. Interferon-γ is an important proinflammatory cytokine in the interleukin-12 cascade and has been implicated in the pathogenesis of mucosal inflammation. We tested this gene for evidence of linkage and association in 133 German multiplex families and 506 single patients with their parents. An intragenic, highly informative CA-repeat marker in intron 1 of the gene was typed using fluorescence-labeled polymerase chain reaction and analysis on an automated sequencer. In the nonparametric linkage analysis using GENEHUNTER, a nonsignificant maximum LOD score of 0.67 was obtained. The transmission disequilibrium test for association was negative (P≥0.22) for Crohn's disease, ulcerative colitis, and the combined inflammatory bowel disease phenotype. In summary, the findings make interferon-γ a very unlikely candidate for the major susceptibility gene in the chromosome 12 linkage interval. Future efforts can concentrate on other transcripts in the region.
1432-1262
14321262
Springer
shingle_catch_all_2 Hampe, J.
Hermann, B.
Bridger, S.
MacPherson, A. J. S.
Mathew, C. G.
Schreiber, S.
The interferon-Á gene as a positional and functional candidate gene for inflammatory bowel disease
Key words Pro-inflammatory cytokines
Crohn's disease
Ulcerative colitis
Susceptibility
Key words Pro-inflammatory cytokines
Crohn's disease
Ulcerative colitis
Susceptibility
Abstract Epidemiological and genome-wide linkage analyses have provided firm evidence for a genetic component in the pathogenesis of inflammatory bowel disease. The linkage regions on chromosomes 12 and 16 have been replicated in several independent samples. These represent the best positional evidence in the search for inflammatory bowel disease susceptibility genes. While systematic association and physical mapping studies in these regions are under way, the direct analysis of immunologically relevant genes as positional and functional candidates may provide a shortcut in this process. The interferon-γ gene resides in the chromosome 12 linkage region near the marker D12S83. Interferon-γ is an important proinflammatory cytokine in the interleukin-12 cascade and has been implicated in the pathogenesis of mucosal inflammation. We tested this gene for evidence of linkage and association in 133 German multiplex families and 506 single patients with their parents. An intragenic, highly informative CA-repeat marker in intron 1 of the gene was typed using fluorescence-labeled polymerase chain reaction and analysis on an automated sequencer. In the nonparametric linkage analysis using GENEHUNTER, a nonsignificant maximum LOD score of 0.67 was obtained. The transmission disequilibrium test for association was negative (P≥0.22) for Crohn's disease, ulcerative colitis, and the combined inflammatory bowel disease phenotype. In summary, the findings make interferon-γ a very unlikely candidate for the major susceptibility gene in the chromosome 12 linkage interval. Future efforts can concentrate on other transcripts in the region.
1432-1262
14321262
Springer
shingle_catch_all_3 Hampe, J.
Hermann, B.
Bridger, S.
MacPherson, A. J. S.
Mathew, C. G.
Schreiber, S.
The interferon-Á gene as a positional and functional candidate gene for inflammatory bowel disease
Key words Pro-inflammatory cytokines
Crohn's disease
Ulcerative colitis
Susceptibility
Key words Pro-inflammatory cytokines
Crohn's disease
Ulcerative colitis
Susceptibility
Abstract Epidemiological and genome-wide linkage analyses have provided firm evidence for a genetic component in the pathogenesis of inflammatory bowel disease. The linkage regions on chromosomes 12 and 16 have been replicated in several independent samples. These represent the best positional evidence in the search for inflammatory bowel disease susceptibility genes. While systematic association and physical mapping studies in these regions are under way, the direct analysis of immunologically relevant genes as positional and functional candidates may provide a shortcut in this process. The interferon-γ gene resides in the chromosome 12 linkage region near the marker D12S83. Interferon-γ is an important proinflammatory cytokine in the interleukin-12 cascade and has been implicated in the pathogenesis of mucosal inflammation. We tested this gene for evidence of linkage and association in 133 German multiplex families and 506 single patients with their parents. An intragenic, highly informative CA-repeat marker in intron 1 of the gene was typed using fluorescence-labeled polymerase chain reaction and analysis on an automated sequencer. In the nonparametric linkage analysis using GENEHUNTER, a nonsignificant maximum LOD score of 0.67 was obtained. The transmission disequilibrium test for association was negative (P≥0.22) for Crohn's disease, ulcerative colitis, and the combined inflammatory bowel disease phenotype. In summary, the findings make interferon-γ a very unlikely candidate for the major susceptibility gene in the chromosome 12 linkage interval. Future efforts can concentrate on other transcripts in the region.
1432-1262
14321262
Springer
shingle_catch_all_4 Hampe, J.
Hermann, B.
Bridger, S.
MacPherson, A. J. S.
Mathew, C. G.
Schreiber, S.
The interferon-Á gene as a positional and functional candidate gene for inflammatory bowel disease
Key words Pro-inflammatory cytokines
Crohn's disease
Ulcerative colitis
Susceptibility
Key words Pro-inflammatory cytokines
Crohn's disease
Ulcerative colitis
Susceptibility
Abstract Epidemiological and genome-wide linkage analyses have provided firm evidence for a genetic component in the pathogenesis of inflammatory bowel disease. The linkage regions on chromosomes 12 and 16 have been replicated in several independent samples. These represent the best positional evidence in the search for inflammatory bowel disease susceptibility genes. While systematic association and physical mapping studies in these regions are under way, the direct analysis of immunologically relevant genes as positional and functional candidates may provide a shortcut in this process. The interferon-γ gene resides in the chromosome 12 linkage region near the marker D12S83. Interferon-γ is an important proinflammatory cytokine in the interleukin-12 cascade and has been implicated in the pathogenesis of mucosal inflammation. We tested this gene for evidence of linkage and association in 133 German multiplex families and 506 single patients with their parents. An intragenic, highly informative CA-repeat marker in intron 1 of the gene was typed using fluorescence-labeled polymerase chain reaction and analysis on an automated sequencer. In the nonparametric linkage analysis using GENEHUNTER, a nonsignificant maximum LOD score of 0.67 was obtained. The transmission disequilibrium test for association was negative (P≥0.22) for Crohn's disease, ulcerative colitis, and the combined inflammatory bowel disease phenotype. In summary, the findings make interferon-γ a very unlikely candidate for the major susceptibility gene in the chromosome 12 linkage interval. Future efforts can concentrate on other transcripts in the region.
1432-1262
14321262
Springer
shingle_title_1 The interferon-Á gene as a positional and functional candidate gene for inflammatory bowel disease
shingle_title_2 The interferon-Á gene as a positional and functional candidate gene for inflammatory bowel disease
shingle_title_3 The interferon-Á gene as a positional and functional candidate gene for inflammatory bowel disease
shingle_title_4 The interferon-Á gene as a positional and functional candidate gene for inflammatory bowel disease
sigel_instance_filter dkfz
geomar
wilbert
ipn
albert
fhp
source_archive Springer Online Journal Archives 1860-2000
timestamp 2024-05-06T09:39:27.343Z
titel The interferon-Á gene as a positional and functional candidate gene for inflammatory bowel disease
titel_suche The interferon-Á gene as a positional and functional candidate gene for inflammatory bowel disease
topic WW-YZ
uid nat_lic_papers_NLM200835661