Mesophilic syntrophic acetate oxidation during methane formation by a triculture at high ammonium concentration
| ISSN: |
1432-072X
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| Keywords: |
Mesophilic acetate oxidation ; Methanogenesis ; Ammonium ; Syntrophy ; Interspecies hydrogen transfer
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| Source: |
Springer Online Journal Archives 1860-2000
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| Topics: |
Biology
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| Notes: |
Abstract In a mesophilic (37°C) triculture at a high ammonium concentration and pH8, methanogenesis from acetate occurred via syntrophic acetate oxidation. Studies with 14C-labelled substrates showed that the amount of labelled methane formed from 1-14C-labelled acetate was equal to that formed from 2-14C-labelled acetate. Labelled methane was also formed from H14CO3 -. These results clearly showed that both the methyl and carboxyl groups of acetate were oxidized to CO2 and that CO2 was reduced to CH4 through hydrogenotrophic methanogenesis. During growth of the triculture, a significant isotopic exchange between the carboxyl group of acetate and bicarbonate occurred. As a result, there was a decrease in the specific activity of 1-14C-acetate, and the production of 14CO2 was slightly higher from 1-14C- than from 2-14C-acetate. For each mole acetate degraded, 0.94 mol methane was formed; 9.2 mmol acetate was metabolized during the 294 days of incubation.
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| Type of Medium: |
Electronic Resource
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| URL: |
| _version_ | 1798295490947710976 |
|---|---|
| autor | Schnürer, Anna Houwen, Frans P. Svensson, Bo H. |
| autorsonst | Schnürer, Anna Houwen, Frans P. Svensson, Bo H. |
| book_url | http://dx.doi.org/10.1007/BF00264375 |
| datenlieferant | nat_lic_papers |
| hauptsatz | hsatz_simple |
| identnr | NLM200190393 |
| issn | 1432-072X |
| journal_name | Archives of microbiology |
| materialart | 1 |
| notes | Abstract In a mesophilic (37°C) triculture at a high ammonium concentration and pH8, methanogenesis from acetate occurred via syntrophic acetate oxidation. Studies with 14C-labelled substrates showed that the amount of labelled methane formed from 1-14C-labelled acetate was equal to that formed from 2-14C-labelled acetate. Labelled methane was also formed from H14CO3 -. These results clearly showed that both the methyl and carboxyl groups of acetate were oxidized to CO2 and that CO2 was reduced to CH4 through hydrogenotrophic methanogenesis. During growth of the triculture, a significant isotopic exchange between the carboxyl group of acetate and bicarbonate occurred. As a result, there was a decrease in the specific activity of 1-14C-acetate, and the production of 14CO2 was slightly higher from 1-14C- than from 2-14C-acetate. For each mole acetate degraded, 0.94 mol methane was formed; 9.2 mmol acetate was metabolized during the 294 days of incubation. |
| package_name | Springer |
| publikationsjahr_anzeige | 1994 |
| publikationsjahr_facette | 1994 |
| publikationsjahr_intervall | 8009:1990-1994 |
| publikationsjahr_sort | 1994 |
| publisher | Springer |
| reference | 162 (1994), S. 70-74 |
| schlagwort | Mesophilic acetate oxidation Methanogenesis Ammonium Syntrophy Interspecies hydrogen transfer |
| search_space | articles |
| shingle_author_1 | Schnürer, Anna Houwen, Frans P. Svensson, Bo H. |
| shingle_author_2 | Schnürer, Anna Houwen, Frans P. Svensson, Bo H. |
| shingle_author_3 | Schnürer, Anna Houwen, Frans P. Svensson, Bo H. |
| shingle_author_4 | Schnürer, Anna Houwen, Frans P. Svensson, Bo H. |
| shingle_catch_all_1 | Schnürer, Anna Houwen, Frans P. Svensson, Bo H. Mesophilic syntrophic acetate oxidation during methane formation by a triculture at high ammonium concentration Mesophilic acetate oxidation Methanogenesis Ammonium Syntrophy Interspecies hydrogen transfer Mesophilic acetate oxidation Methanogenesis Ammonium Syntrophy Interspecies hydrogen transfer Abstract In a mesophilic (37°C) triculture at a high ammonium concentration and pH8, methanogenesis from acetate occurred via syntrophic acetate oxidation. Studies with 14C-labelled substrates showed that the amount of labelled methane formed from 1-14C-labelled acetate was equal to that formed from 2-14C-labelled acetate. Labelled methane was also formed from H14CO3 -. These results clearly showed that both the methyl and carboxyl groups of acetate were oxidized to CO2 and that CO2 was reduced to CH4 through hydrogenotrophic methanogenesis. During growth of the triculture, a significant isotopic exchange between the carboxyl group of acetate and bicarbonate occurred. As a result, there was a decrease in the specific activity of 1-14C-acetate, and the production of 14CO2 was slightly higher from 1-14C- than from 2-14C-acetate. For each mole acetate degraded, 0.94 mol methane was formed; 9.2 mmol acetate was metabolized during the 294 days of incubation. 1432-072X 1432072X Springer |
| shingle_catch_all_2 | Schnürer, Anna Houwen, Frans P. Svensson, Bo H. Mesophilic syntrophic acetate oxidation during methane formation by a triculture at high ammonium concentration Mesophilic acetate oxidation Methanogenesis Ammonium Syntrophy Interspecies hydrogen transfer Mesophilic acetate oxidation Methanogenesis Ammonium Syntrophy Interspecies hydrogen transfer Abstract In a mesophilic (37°C) triculture at a high ammonium concentration and pH8, methanogenesis from acetate occurred via syntrophic acetate oxidation. Studies with 14C-labelled substrates showed that the amount of labelled methane formed from 1-14C-labelled acetate was equal to that formed from 2-14C-labelled acetate. Labelled methane was also formed from H14CO3 -. These results clearly showed that both the methyl and carboxyl groups of acetate were oxidized to CO2 and that CO2 was reduced to CH4 through hydrogenotrophic methanogenesis. During growth of the triculture, a significant isotopic exchange between the carboxyl group of acetate and bicarbonate occurred. As a result, there was a decrease in the specific activity of 1-14C-acetate, and the production of 14CO2 was slightly higher from 1-14C- than from 2-14C-acetate. For each mole acetate degraded, 0.94 mol methane was formed; 9.2 mmol acetate was metabolized during the 294 days of incubation. 1432-072X 1432072X Springer |
| shingle_catch_all_3 | Schnürer, Anna Houwen, Frans P. Svensson, Bo H. Mesophilic syntrophic acetate oxidation during methane formation by a triculture at high ammonium concentration Mesophilic acetate oxidation Methanogenesis Ammonium Syntrophy Interspecies hydrogen transfer Mesophilic acetate oxidation Methanogenesis Ammonium Syntrophy Interspecies hydrogen transfer Abstract In a mesophilic (37°C) triculture at a high ammonium concentration and pH8, methanogenesis from acetate occurred via syntrophic acetate oxidation. Studies with 14C-labelled substrates showed that the amount of labelled methane formed from 1-14C-labelled acetate was equal to that formed from 2-14C-labelled acetate. Labelled methane was also formed from H14CO3 -. These results clearly showed that both the methyl and carboxyl groups of acetate were oxidized to CO2 and that CO2 was reduced to CH4 through hydrogenotrophic methanogenesis. During growth of the triculture, a significant isotopic exchange between the carboxyl group of acetate and bicarbonate occurred. As a result, there was a decrease in the specific activity of 1-14C-acetate, and the production of 14CO2 was slightly higher from 1-14C- than from 2-14C-acetate. For each mole acetate degraded, 0.94 mol methane was formed; 9.2 mmol acetate was metabolized during the 294 days of incubation. 1432-072X 1432072X Springer |
| shingle_catch_all_4 | Schnürer, Anna Houwen, Frans P. Svensson, Bo H. Mesophilic syntrophic acetate oxidation during methane formation by a triculture at high ammonium concentration Mesophilic acetate oxidation Methanogenesis Ammonium Syntrophy Interspecies hydrogen transfer Mesophilic acetate oxidation Methanogenesis Ammonium Syntrophy Interspecies hydrogen transfer Abstract In a mesophilic (37°C) triculture at a high ammonium concentration and pH8, methanogenesis from acetate occurred via syntrophic acetate oxidation. Studies with 14C-labelled substrates showed that the amount of labelled methane formed from 1-14C-labelled acetate was equal to that formed from 2-14C-labelled acetate. Labelled methane was also formed from H14CO3 -. These results clearly showed that both the methyl and carboxyl groups of acetate were oxidized to CO2 and that CO2 was reduced to CH4 through hydrogenotrophic methanogenesis. During growth of the triculture, a significant isotopic exchange between the carboxyl group of acetate and bicarbonate occurred. As a result, there was a decrease in the specific activity of 1-14C-acetate, and the production of 14CO2 was slightly higher from 1-14C- than from 2-14C-acetate. For each mole acetate degraded, 0.94 mol methane was formed; 9.2 mmol acetate was metabolized during the 294 days of incubation. 1432-072X 1432072X Springer |
| shingle_title_1 | Mesophilic syntrophic acetate oxidation during methane formation by a triculture at high ammonium concentration |
| shingle_title_2 | Mesophilic syntrophic acetate oxidation during methane formation by a triculture at high ammonium concentration |
| shingle_title_3 | Mesophilic syntrophic acetate oxidation during methane formation by a triculture at high ammonium concentration |
| shingle_title_4 | Mesophilic syntrophic acetate oxidation during methane formation by a triculture at high ammonium concentration |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Springer Online Journal Archives 1860-2000 |
| timestamp | 2024-05-06T09:37:02.802Z |
| titel | Mesophilic syntrophic acetate oxidation during methane formation by a triculture at high ammonium concentration |
| titel_suche | Mesophilic syntrophic acetate oxidation during methane formation by a triculture at high ammonium concentration |
| topic | W |
| uid | nat_lic_papers_NLM200190393 |