Formate-limited growth of Methanobacterium formicium in steady-state cultures
| ISSN: |
1432-072X
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| Keywords: |
Methanobacterium formicicum ; Chemostat ; Growth parameters ; Hydrogenase ; Formate dehydrogenase
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| Source: |
Springer Online Journal Archives 1860-2000
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| Topics: |
Biology
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| Notes: |
Abstract Growth of Methanobacterium formicicum strain MF was studied in pH-stat batch cultures and formatelimited chemostat cultures. The maxium rate of methanogenesis from formate occurred at pH 7.6 and 43°C and the maximum specific growth rate constant (μm) was 0.08 h-1. The K s and maximum growth yield (Y s max ) were 3.5 mM formate and 1.4 (g dry wt) mol01 formate respectively, and the maintenance coefficient (m) was calculated as 6.8 mmol formate (g dry wt)-1 h-1. The efficiency of electron transport phosphorylation during formate metabolism assuming Y ATP of 10.5 g mol-1 was about 20%. The specific activities of hydrogenase and formate dehydrogenase increased slightly with dilution rate.
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| Type of Medium: |
Electronic Resource
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| URL: |