The beta cell glucokinase promoter variant is an unlikely risk factor for diabetes mellitus
Lotfi, K. ; Sund, G. ; Lowe, R. ; Graham, J. ; Landin-Olsson, M. ; Kockum, I. ; Deeb, S. ; Lernmark, Å.
Springer
Published 1997
Springer
Published 1997
| ISSN: |
1432-0428
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|---|---|
| Keywords: |
Keywords IDDM ; NIDDM ; glucokinase gene ; polymerase chain reaction ; single strand conformational polymorphism ; islet cell antibodies ; GAD65 antibodies.
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| Source: |
Springer Online Journal Archives 1860-2000
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| Topics: |
Medicine
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| Notes: |
Summary Glucokinase plays an important role in the regulation of insulin secretion and is therefore an attractive candidate gene for both insulin dependent (IDDM) and non-insulin-dependent (NIDDM) diabetes mellitus. A single G-A nucleotide polymorphism at the –30 position of the beta-cell specific promoter region of the glucokinase gene was previously associated with reduced beta-cell function. In the present study we analysed 268 consecutive newly diagnosed Swedish patients classified with either IDDM (n = 205), NIDDM (n = 31) or unclassifiable (n = 32) diabetes between the ages of 15 and 35 years along with a group of 158 age- and sex-matched control subjects. The beta-cell promoter region was amplified by the polymerase chain reaction and the G-A variant identified by single strand conformational polymorphism. There was no significant difference in allele frequencies of G and A between any of the subject groups and likewise, no significant difference in the frequencies of the G/G, G/A, or A/A genotypes. Eight subjects were homozygous for the less common A allele, five had IDDM and three were control subjects. Our results suggest that the –30 beta-cell glucokinase promoter variant is not associated with IDDM. [Diabetologia (1997) 40: 959–962)
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| Type of Medium: |
Electronic Resource
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| URL: |
| _version_ | 1798295381518319616 |
|---|---|
| autor | Lotfi, K. Sund, G. Lowe, R. Graham, J. Landin-Olsson, M. Kockum, I. Deeb, S. Lernmark, Å. |
| autorsonst | Lotfi, K. Sund, G. Lowe, R. Graham, J. Landin-Olsson, M. Kockum, I. Deeb, S. Lernmark, Å. |
| book_url | http://dx.doi.org/10.1007/s001250050774 |
| datenlieferant | nat_lic_papers |
| hauptsatz | hsatz_simple |
| identnr | NLM199942048 |
| issn | 1432-0428 |
| journal_name | Diabetologia |
| materialart | 1 |
| notes | Summary Glucokinase plays an important role in the regulation of insulin secretion and is therefore an attractive candidate gene for both insulin dependent (IDDM) and non-insulin-dependent (NIDDM) diabetes mellitus. A single G-A nucleotide polymorphism at the –30 position of the beta-cell specific promoter region of the glucokinase gene was previously associated with reduced beta-cell function. In the present study we analysed 268 consecutive newly diagnosed Swedish patients classified with either IDDM (n = 205), NIDDM (n = 31) or unclassifiable (n = 32) diabetes between the ages of 15 and 35 years along with a group of 158 age- and sex-matched control subjects. The beta-cell promoter region was amplified by the polymerase chain reaction and the G-A variant identified by single strand conformational polymorphism. There was no significant difference in allele frequencies of G and A between any of the subject groups and likewise, no significant difference in the frequencies of the G/G, G/A, or A/A genotypes. Eight subjects were homozygous for the less common A allele, five had IDDM and three were control subjects. Our results suggest that the –30 beta-cell glucokinase promoter variant is not associated with IDDM. [Diabetologia (1997) 40: 959–962) |
| package_name | Springer |
| publikationsjahr_anzeige | 1997 |
| publikationsjahr_facette | 1997 |
| publikationsjahr_intervall | 8004:1995-1999 |
| publikationsjahr_sort | 1997 |
| publisher | Springer |
| reference | 40 (1997), S. 959-962 |
| schlagwort | Keywords IDDM NIDDM glucokinase gene polymerase chain reaction single strand conformational polymorphism islet cell antibodies GAD65 antibodies. |
| search_space | articles |
| shingle_author_1 | Lotfi, K. Sund, G. Lowe, R. Graham, J. Landin-Olsson, M. Kockum, I. Deeb, S. Lernmark, Å. |
| shingle_author_2 | Lotfi, K. Sund, G. Lowe, R. Graham, J. Landin-Olsson, M. Kockum, I. Deeb, S. Lernmark, Å. |
| shingle_author_3 | Lotfi, K. Sund, G. Lowe, R. Graham, J. Landin-Olsson, M. Kockum, I. Deeb, S. Lernmark, Å. |
| shingle_author_4 | Lotfi, K. Sund, G. Lowe, R. Graham, J. Landin-Olsson, M. Kockum, I. Deeb, S. Lernmark, Å. |
| shingle_catch_all_1 | Lotfi, K. Sund, G. Lowe, R. Graham, J. Landin-Olsson, M. Kockum, I. Deeb, S. Lernmark, Å. The beta cell glucokinase promoter variant is an unlikely risk factor for diabetes mellitus Keywords IDDM NIDDM glucokinase gene polymerase chain reaction single strand conformational polymorphism islet cell antibodies GAD65 antibodies. Keywords IDDM NIDDM glucokinase gene polymerase chain reaction single strand conformational polymorphism islet cell antibodies GAD65 antibodies. Summary Glucokinase plays an important role in the regulation of insulin secretion and is therefore an attractive candidate gene for both insulin dependent (IDDM) and non-insulin-dependent (NIDDM) diabetes mellitus. A single G-A nucleotide polymorphism at the –30 position of the beta-cell specific promoter region of the glucokinase gene was previously associated with reduced beta-cell function. In the present study we analysed 268 consecutive newly diagnosed Swedish patients classified with either IDDM (n = 205), NIDDM (n = 31) or unclassifiable (n = 32) diabetes between the ages of 15 and 35 years along with a group of 158 age- and sex-matched control subjects. The beta-cell promoter region was amplified by the polymerase chain reaction and the G-A variant identified by single strand conformational polymorphism. There was no significant difference in allele frequencies of G and A between any of the subject groups and likewise, no significant difference in the frequencies of the G/G, G/A, or A/A genotypes. Eight subjects were homozygous for the less common A allele, five had IDDM and three were control subjects. Our results suggest that the –30 beta-cell glucokinase promoter variant is not associated with IDDM. [Diabetologia (1997) 40: 959–962) 1432-0428 14320428 Springer |
| shingle_catch_all_2 | Lotfi, K. Sund, G. Lowe, R. Graham, J. Landin-Olsson, M. Kockum, I. Deeb, S. Lernmark, Å. The beta cell glucokinase promoter variant is an unlikely risk factor for diabetes mellitus Keywords IDDM NIDDM glucokinase gene polymerase chain reaction single strand conformational polymorphism islet cell antibodies GAD65 antibodies. Keywords IDDM NIDDM glucokinase gene polymerase chain reaction single strand conformational polymorphism islet cell antibodies GAD65 antibodies. Summary Glucokinase plays an important role in the regulation of insulin secretion and is therefore an attractive candidate gene for both insulin dependent (IDDM) and non-insulin-dependent (NIDDM) diabetes mellitus. A single G-A nucleotide polymorphism at the –30 position of the beta-cell specific promoter region of the glucokinase gene was previously associated with reduced beta-cell function. In the present study we analysed 268 consecutive newly diagnosed Swedish patients classified with either IDDM (n = 205), NIDDM (n = 31) or unclassifiable (n = 32) diabetes between the ages of 15 and 35 years along with a group of 158 age- and sex-matched control subjects. The beta-cell promoter region was amplified by the polymerase chain reaction and the G-A variant identified by single strand conformational polymorphism. There was no significant difference in allele frequencies of G and A between any of the subject groups and likewise, no significant difference in the frequencies of the G/G, G/A, or A/A genotypes. Eight subjects were homozygous for the less common A allele, five had IDDM and three were control subjects. Our results suggest that the –30 beta-cell glucokinase promoter variant is not associated with IDDM. [Diabetologia (1997) 40: 959–962) 1432-0428 14320428 Springer |
| shingle_catch_all_3 | Lotfi, K. Sund, G. Lowe, R. Graham, J. Landin-Olsson, M. Kockum, I. Deeb, S. Lernmark, Å. The beta cell glucokinase promoter variant is an unlikely risk factor for diabetes mellitus Keywords IDDM NIDDM glucokinase gene polymerase chain reaction single strand conformational polymorphism islet cell antibodies GAD65 antibodies. Keywords IDDM NIDDM glucokinase gene polymerase chain reaction single strand conformational polymorphism islet cell antibodies GAD65 antibodies. Summary Glucokinase plays an important role in the regulation of insulin secretion and is therefore an attractive candidate gene for both insulin dependent (IDDM) and non-insulin-dependent (NIDDM) diabetes mellitus. A single G-A nucleotide polymorphism at the –30 position of the beta-cell specific promoter region of the glucokinase gene was previously associated with reduced beta-cell function. In the present study we analysed 268 consecutive newly diagnosed Swedish patients classified with either IDDM (n = 205), NIDDM (n = 31) or unclassifiable (n = 32) diabetes between the ages of 15 and 35 years along with a group of 158 age- and sex-matched control subjects. The beta-cell promoter region was amplified by the polymerase chain reaction and the G-A variant identified by single strand conformational polymorphism. There was no significant difference in allele frequencies of G and A between any of the subject groups and likewise, no significant difference in the frequencies of the G/G, G/A, or A/A genotypes. Eight subjects were homozygous for the less common A allele, five had IDDM and three were control subjects. Our results suggest that the –30 beta-cell glucokinase promoter variant is not associated with IDDM. [Diabetologia (1997) 40: 959–962) 1432-0428 14320428 Springer |
| shingle_catch_all_4 | Lotfi, K. Sund, G. Lowe, R. Graham, J. Landin-Olsson, M. Kockum, I. Deeb, S. Lernmark, Å. The beta cell glucokinase promoter variant is an unlikely risk factor for diabetes mellitus Keywords IDDM NIDDM glucokinase gene polymerase chain reaction single strand conformational polymorphism islet cell antibodies GAD65 antibodies. Keywords IDDM NIDDM glucokinase gene polymerase chain reaction single strand conformational polymorphism islet cell antibodies GAD65 antibodies. Summary Glucokinase plays an important role in the regulation of insulin secretion and is therefore an attractive candidate gene for both insulin dependent (IDDM) and non-insulin-dependent (NIDDM) diabetes mellitus. A single G-A nucleotide polymorphism at the –30 position of the beta-cell specific promoter region of the glucokinase gene was previously associated with reduced beta-cell function. In the present study we analysed 268 consecutive newly diagnosed Swedish patients classified with either IDDM (n = 205), NIDDM (n = 31) or unclassifiable (n = 32) diabetes between the ages of 15 and 35 years along with a group of 158 age- and sex-matched control subjects. The beta-cell promoter region was amplified by the polymerase chain reaction and the G-A variant identified by single strand conformational polymorphism. There was no significant difference in allele frequencies of G and A between any of the subject groups and likewise, no significant difference in the frequencies of the G/G, G/A, or A/A genotypes. Eight subjects were homozygous for the less common A allele, five had IDDM and three were control subjects. Our results suggest that the –30 beta-cell glucokinase promoter variant is not associated with IDDM. [Diabetologia (1997) 40: 959–962) 1432-0428 14320428 Springer |
| shingle_title_1 | The beta cell glucokinase promoter variant is an unlikely risk factor for diabetes mellitus |
| shingle_title_2 | The beta cell glucokinase promoter variant is an unlikely risk factor for diabetes mellitus |
| shingle_title_3 | The beta cell glucokinase promoter variant is an unlikely risk factor for diabetes mellitus |
| shingle_title_4 | The beta cell glucokinase promoter variant is an unlikely risk factor for diabetes mellitus |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Springer Online Journal Archives 1860-2000 |
| timestamp | 2024-05-06T09:35:18.010Z |
| titel | The beta cell glucokinase promoter variant is an unlikely risk factor for diabetes mellitus |
| titel_suche | The beta cell glucokinase promoter variant is an unlikely risk factor for diabetes mellitus |
| topic | WW-YZ |
| uid | nat_lic_papers_NLM199942048 |