Low levels of DNA sequence variation among adapted genotypes of hexaploid wheat
Bryan, G. J. ; Stephenson, P. ; Collins, A. ; Kirby, J. ; Smith, J. B. ; Gale, M. D.
Springer
Published 1999
Springer
Published 1999
| ISSN: |
1432-2242
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|---|---|
| Keywords: |
Key words PCR markers ; Sequence-tagged-site ; Wheat
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| Source: |
Springer Online Journal Archives 1860-2000
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| Topics: |
Biology
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| Notes: |
Abstract PCR products from regions corresponding to sequences hybridising to wheat RFLP probes were sequenced in order to establish the level of DNA sequence variation among adapted wheat genotypes. Hexaploid bread wheat shows a very low rate of nucleotide polymorphism, approximately 1 polymorphic nucleotide per 1000 basepairs. Differences in PCR product length can be exploited to design genome-specific amplicons, which may have use in gene tagging or in diagnostic applications. Interpretation of results may be complicated by the simultaneous amplification of orthologous and paralogous sequences. These findings have significant implications for the use of STS markers in wheat and other polyploid species.
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| Type of Medium: |
Electronic Resource
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| URL: |