Characterization and localization of repetitive DNA sequences in the ornamental Alstroemeria aurea Graham
Jeu, M. J. De ; Lasschuit, J. ; Kuipers, A. G. J. ; Kamstra, S. A. ; Visser, R. G. F.
Springer
Published 1997
Springer
Published 1997
| ISSN: |
1432-2242
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|---|---|
| Keywords: |
Key words Alstroemeria aurea ; Repetitive DNA ; FISH-fluorescence in situ hybridization ; Heterochromatin ; Phylogeny
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| Source: |
Springer Online Journal Archives 1860-2000
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| Topics: |
Biology
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| Notes: |
Abstract Three repetitive DNA sequences were isolated from a genomic DNA library of the ornamental Alstroemeria aurea Graham. Two repeats, A001-I and A001-II, were quite homologous and highly A. aurea-specific. A001-I was a 217-bp sequence with several telomeric TTTAGGG repeats at the 5′ end and a unique sequence of 98 bp at the other end. The third repeat, A001-IV, was a 840-bp sequence which contained two sub-sequences of 56 and 74 bp respectively, previously found in chloroplast (cp) DNA of tobacco and spinach and to a lesser extent in the cpDNA of maize and rice. Repeat A001-IV was not species-specific and its hybridization signal was weaker than the other repeats. Fluorescence in situ hybridization (FISH) revealed the A. aurea-specific repeats to be located in the heterochromatic regions of all A. aurea chromosomes. The differences in FISH pattern make them useful tools for karyotype analysis. The non-species-specific sequence A001-IV gave a dispersed signal over all the Alstroemeria chromosomes in an interspecific hybrid. The potential use of these repetitive DNA sequences for the study of phylogenetic relationships within the genus Alstroemeria is discussed.
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| Type of Medium: |
Electronic Resource
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| URL: |
| _version_ | 1798295972071079936 |
|---|---|
| autor | Jeu, M. J. De Lasschuit, J. Kuipers, A. G. J. Kamstra, S. A. Visser, R. G. F. |
| autorsonst | Jeu, M. J. De Lasschuit, J. Kuipers, A. G. J. Kamstra, S. A. Visser, R. G. F. |
| book_url | http://dx.doi.org/10.1007/s001220050505 |
| datenlieferant | nat_lic_papers |
| hauptsatz | hsatz_simple |
| identnr | NLM199880409 |
| issn | 1432-2242 |
| journal_name | Theoretical and applied genetics |
| materialart | 1 |
| notes | Abstract Three repetitive DNA sequences were isolated from a genomic DNA library of the ornamental Alstroemeria aurea Graham. Two repeats, A001-I and A001-II, were quite homologous and highly A. aurea-specific. A001-I was a 217-bp sequence with several telomeric TTTAGGG repeats at the 5′ end and a unique sequence of 98 bp at the other end. The third repeat, A001-IV, was a 840-bp sequence which contained two sub-sequences of 56 and 74 bp respectively, previously found in chloroplast (cp) DNA of tobacco and spinach and to a lesser extent in the cpDNA of maize and rice. Repeat A001-IV was not species-specific and its hybridization signal was weaker than the other repeats. Fluorescence in situ hybridization (FISH) revealed the A. aurea-specific repeats to be located in the heterochromatic regions of all A. aurea chromosomes. The differences in FISH pattern make them useful tools for karyotype analysis. The non-species-specific sequence A001-IV gave a dispersed signal over all the Alstroemeria chromosomes in an interspecific hybrid. The potential use of these repetitive DNA sequences for the study of phylogenetic relationships within the genus Alstroemeria is discussed. |
| package_name | Springer |
| publikationsjahr_anzeige | 1997 |
| publikationsjahr_facette | 1997 |
| publikationsjahr_intervall | 8004:1995-1999 |
| publikationsjahr_sort | 1997 |
| publisher | Springer |
| reference | 94 (1997), S. 982-990 |
| schlagwort | Key words Alstroemeria aurea Repetitive DNA FISH-fluorescence in situ hybridization Heterochromatin Phylogeny |
| search_space | articles |
| shingle_author_1 | Jeu, M. J. De Lasschuit, J. Kuipers, A. G. J. Kamstra, S. A. Visser, R. G. F. |
| shingle_author_2 | Jeu, M. J. De Lasschuit, J. Kuipers, A. G. J. Kamstra, S. A. Visser, R. G. F. |
| shingle_author_3 | Jeu, M. J. De Lasschuit, J. Kuipers, A. G. J. Kamstra, S. A. Visser, R. G. F. |
| shingle_author_4 | Jeu, M. J. De Lasschuit, J. Kuipers, A. G. J. Kamstra, S. A. Visser, R. G. F. |
| shingle_catch_all_1 | Jeu, M. J. De Lasschuit, J. Kuipers, A. G. J. Kamstra, S. A. Visser, R. G. F. Characterization and localization of repetitive DNA sequences in the ornamental Alstroemeria aurea Graham Key words Alstroemeria aurea Repetitive DNA FISH-fluorescence in situ hybridization Heterochromatin Phylogeny Key words Alstroemeria aurea Repetitive DNA FISH-fluorescence in situ hybridization Heterochromatin Phylogeny Abstract Three repetitive DNA sequences were isolated from a genomic DNA library of the ornamental Alstroemeria aurea Graham. Two repeats, A001-I and A001-II, were quite homologous and highly A. aurea-specific. A001-I was a 217-bp sequence with several telomeric TTTAGGG repeats at the 5′ end and a unique sequence of 98 bp at the other end. The third repeat, A001-IV, was a 840-bp sequence which contained two sub-sequences of 56 and 74 bp respectively, previously found in chloroplast (cp) DNA of tobacco and spinach and to a lesser extent in the cpDNA of maize and rice. Repeat A001-IV was not species-specific and its hybridization signal was weaker than the other repeats. Fluorescence in situ hybridization (FISH) revealed the A. aurea-specific repeats to be located in the heterochromatic regions of all A. aurea chromosomes. The differences in FISH pattern make them useful tools for karyotype analysis. The non-species-specific sequence A001-IV gave a dispersed signal over all the Alstroemeria chromosomes in an interspecific hybrid. The potential use of these repetitive DNA sequences for the study of phylogenetic relationships within the genus Alstroemeria is discussed. 1432-2242 14322242 Springer |
| shingle_catch_all_2 | Jeu, M. J. De Lasschuit, J. Kuipers, A. G. J. Kamstra, S. A. Visser, R. G. F. Characterization and localization of repetitive DNA sequences in the ornamental Alstroemeria aurea Graham Key words Alstroemeria aurea Repetitive DNA FISH-fluorescence in situ hybridization Heterochromatin Phylogeny Key words Alstroemeria aurea Repetitive DNA FISH-fluorescence in situ hybridization Heterochromatin Phylogeny Abstract Three repetitive DNA sequences were isolated from a genomic DNA library of the ornamental Alstroemeria aurea Graham. Two repeats, A001-I and A001-II, were quite homologous and highly A. aurea-specific. A001-I was a 217-bp sequence with several telomeric TTTAGGG repeats at the 5′ end and a unique sequence of 98 bp at the other end. The third repeat, A001-IV, was a 840-bp sequence which contained two sub-sequences of 56 and 74 bp respectively, previously found in chloroplast (cp) DNA of tobacco and spinach and to a lesser extent in the cpDNA of maize and rice. Repeat A001-IV was not species-specific and its hybridization signal was weaker than the other repeats. Fluorescence in situ hybridization (FISH) revealed the A. aurea-specific repeats to be located in the heterochromatic regions of all A. aurea chromosomes. The differences in FISH pattern make them useful tools for karyotype analysis. The non-species-specific sequence A001-IV gave a dispersed signal over all the Alstroemeria chromosomes in an interspecific hybrid. The potential use of these repetitive DNA sequences for the study of phylogenetic relationships within the genus Alstroemeria is discussed. 1432-2242 14322242 Springer |
| shingle_catch_all_3 | Jeu, M. J. De Lasschuit, J. Kuipers, A. G. J. Kamstra, S. A. Visser, R. G. F. Characterization and localization of repetitive DNA sequences in the ornamental Alstroemeria aurea Graham Key words Alstroemeria aurea Repetitive DNA FISH-fluorescence in situ hybridization Heterochromatin Phylogeny Key words Alstroemeria aurea Repetitive DNA FISH-fluorescence in situ hybridization Heterochromatin Phylogeny Abstract Three repetitive DNA sequences were isolated from a genomic DNA library of the ornamental Alstroemeria aurea Graham. Two repeats, A001-I and A001-II, were quite homologous and highly A. aurea-specific. A001-I was a 217-bp sequence with several telomeric TTTAGGG repeats at the 5′ end and a unique sequence of 98 bp at the other end. The third repeat, A001-IV, was a 840-bp sequence which contained two sub-sequences of 56 and 74 bp respectively, previously found in chloroplast (cp) DNA of tobacco and spinach and to a lesser extent in the cpDNA of maize and rice. Repeat A001-IV was not species-specific and its hybridization signal was weaker than the other repeats. Fluorescence in situ hybridization (FISH) revealed the A. aurea-specific repeats to be located in the heterochromatic regions of all A. aurea chromosomes. The differences in FISH pattern make them useful tools for karyotype analysis. The non-species-specific sequence A001-IV gave a dispersed signal over all the Alstroemeria chromosomes in an interspecific hybrid. The potential use of these repetitive DNA sequences for the study of phylogenetic relationships within the genus Alstroemeria is discussed. 1432-2242 14322242 Springer |
| shingle_catch_all_4 | Jeu, M. J. De Lasschuit, J. Kuipers, A. G. J. Kamstra, S. A. Visser, R. G. F. Characterization and localization of repetitive DNA sequences in the ornamental Alstroemeria aurea Graham Key words Alstroemeria aurea Repetitive DNA FISH-fluorescence in situ hybridization Heterochromatin Phylogeny Key words Alstroemeria aurea Repetitive DNA FISH-fluorescence in situ hybridization Heterochromatin Phylogeny Abstract Three repetitive DNA sequences were isolated from a genomic DNA library of the ornamental Alstroemeria aurea Graham. Two repeats, A001-I and A001-II, were quite homologous and highly A. aurea-specific. A001-I was a 217-bp sequence with several telomeric TTTAGGG repeats at the 5′ end and a unique sequence of 98 bp at the other end. The third repeat, A001-IV, was a 840-bp sequence which contained two sub-sequences of 56 and 74 bp respectively, previously found in chloroplast (cp) DNA of tobacco and spinach and to a lesser extent in the cpDNA of maize and rice. Repeat A001-IV was not species-specific and its hybridization signal was weaker than the other repeats. Fluorescence in situ hybridization (FISH) revealed the A. aurea-specific repeats to be located in the heterochromatic regions of all A. aurea chromosomes. The differences in FISH pattern make them useful tools for karyotype analysis. The non-species-specific sequence A001-IV gave a dispersed signal over all the Alstroemeria chromosomes in an interspecific hybrid. The potential use of these repetitive DNA sequences for the study of phylogenetic relationships within the genus Alstroemeria is discussed. 1432-2242 14322242 Springer |
| shingle_title_1 | Characterization and localization of repetitive DNA sequences in the ornamental Alstroemeria aurea Graham |
| shingle_title_2 | Characterization and localization of repetitive DNA sequences in the ornamental Alstroemeria aurea Graham |
| shingle_title_3 | Characterization and localization of repetitive DNA sequences in the ornamental Alstroemeria aurea Graham |
| shingle_title_4 | Characterization and localization of repetitive DNA sequences in the ornamental Alstroemeria aurea Graham |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Springer Online Journal Archives 1860-2000 |
| timestamp | 2024-05-06T09:44:40.918Z |
| titel | Characterization and localization of repetitive DNA sequences in the ornamental Alstroemeria aurea Graham |
| titel_suche | Characterization and localization of repetitive DNA sequences in the ornamental Alstroemeria aurea Graham |
| topic | W |
| uid | nat_lic_papers_NLM199880409 |