Characterization and localization of repetitive DNA sequences in the ornamental Alstroemeria aurea Graham

ISSN:
1432-2242
Keywords:
Key words Alstroemeria aurea ; Repetitive DNA ; FISH-fluorescence in situ hybridization ; Heterochromatin ; Phylogeny
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract  Three repetitive DNA sequences were isolated from a genomic DNA library of the ornamental Alstroemeria aurea Graham. Two repeats, A001-I and A001-II, were quite homologous and highly A. aurea-specific. A001-I was a 217-bp sequence with several telomeric TTTAGGG repeats at the 5′ end and a unique sequence of 98 bp at the other end. The third repeat, A001-IV, was a 840-bp sequence which contained two sub-sequences of 56 and 74 bp respectively, previously found in chloroplast (cp) DNA of tobacco and spinach and to a lesser extent in the cpDNA of maize and rice. Repeat A001-IV was not species-specific and its hybridization signal was weaker than the other repeats. Fluorescence in situ hybridization (FISH) revealed the A. aurea-specific repeats to be located in the heterochromatic regions of all A. aurea chromosomes. The differences in FISH pattern make them useful tools for karyotype analysis. The non-species-specific sequence A001-IV gave a dispersed signal over all the Alstroemeria chromosomes in an interspecific hybrid. The potential use of these repetitive DNA sequences for the study of phylogenetic relationships within the genus Alstroemeria is discussed.
Type of Medium:
Electronic Resource
URL:
_version_ 1798295972071079936
autor Jeu, M. J. De
Lasschuit, J.
Kuipers, A. G. J.
Kamstra, S. A.
Visser, R. G. F.
autorsonst Jeu, M. J. De
Lasschuit, J.
Kuipers, A. G. J.
Kamstra, S. A.
Visser, R. G. F.
book_url http://dx.doi.org/10.1007/s001220050505
datenlieferant nat_lic_papers
hauptsatz hsatz_simple
identnr NLM199880409
issn 1432-2242
journal_name Theoretical and applied genetics
materialart 1
notes Abstract  Three repetitive DNA sequences were isolated from a genomic DNA library of the ornamental Alstroemeria aurea Graham. Two repeats, A001-I and A001-II, were quite homologous and highly A. aurea-specific. A001-I was a 217-bp sequence with several telomeric TTTAGGG repeats at the 5′ end and a unique sequence of 98 bp at the other end. The third repeat, A001-IV, was a 840-bp sequence which contained two sub-sequences of 56 and 74 bp respectively, previously found in chloroplast (cp) DNA of tobacco and spinach and to a lesser extent in the cpDNA of maize and rice. Repeat A001-IV was not species-specific and its hybridization signal was weaker than the other repeats. Fluorescence in situ hybridization (FISH) revealed the A. aurea-specific repeats to be located in the heterochromatic regions of all A. aurea chromosomes. The differences in FISH pattern make them useful tools for karyotype analysis. The non-species-specific sequence A001-IV gave a dispersed signal over all the Alstroemeria chromosomes in an interspecific hybrid. The potential use of these repetitive DNA sequences for the study of phylogenetic relationships within the genus Alstroemeria is discussed.
package_name Springer
publikationsjahr_anzeige 1997
publikationsjahr_facette 1997
publikationsjahr_intervall 8004:1995-1999
publikationsjahr_sort 1997
publisher Springer
reference 94 (1997), S. 982-990
schlagwort Key words Alstroemeria aurea
Repetitive DNA
FISH-fluorescence in situ hybridization
Heterochromatin
Phylogeny
search_space articles
shingle_author_1 Jeu, M. J. De
Lasschuit, J.
Kuipers, A. G. J.
Kamstra, S. A.
Visser, R. G. F.
shingle_author_2 Jeu, M. J. De
Lasschuit, J.
Kuipers, A. G. J.
Kamstra, S. A.
Visser, R. G. F.
shingle_author_3 Jeu, M. J. De
Lasschuit, J.
Kuipers, A. G. J.
Kamstra, S. A.
Visser, R. G. F.
shingle_author_4 Jeu, M. J. De
Lasschuit, J.
Kuipers, A. G. J.
Kamstra, S. A.
Visser, R. G. F.
shingle_catch_all_1 Jeu, M. J. De
Lasschuit, J.
Kuipers, A. G. J.
Kamstra, S. A.
Visser, R. G. F.
Characterization and localization of repetitive DNA sequences in the ornamental Alstroemeria aurea Graham
Key words Alstroemeria aurea
Repetitive DNA
FISH-fluorescence in situ hybridization
Heterochromatin
Phylogeny
Key words Alstroemeria aurea
Repetitive DNA
FISH-fluorescence in situ hybridization
Heterochromatin
Phylogeny
Abstract  Three repetitive DNA sequences were isolated from a genomic DNA library of the ornamental Alstroemeria aurea Graham. Two repeats, A001-I and A001-II, were quite homologous and highly A. aurea-specific. A001-I was a 217-bp sequence with several telomeric TTTAGGG repeats at the 5′ end and a unique sequence of 98 bp at the other end. The third repeat, A001-IV, was a 840-bp sequence which contained two sub-sequences of 56 and 74 bp respectively, previously found in chloroplast (cp) DNA of tobacco and spinach and to a lesser extent in the cpDNA of maize and rice. Repeat A001-IV was not species-specific and its hybridization signal was weaker than the other repeats. Fluorescence in situ hybridization (FISH) revealed the A. aurea-specific repeats to be located in the heterochromatic regions of all A. aurea chromosomes. The differences in FISH pattern make them useful tools for karyotype analysis. The non-species-specific sequence A001-IV gave a dispersed signal over all the Alstroemeria chromosomes in an interspecific hybrid. The potential use of these repetitive DNA sequences for the study of phylogenetic relationships within the genus Alstroemeria is discussed.
1432-2242
14322242
Springer
shingle_catch_all_2 Jeu, M. J. De
Lasschuit, J.
Kuipers, A. G. J.
Kamstra, S. A.
Visser, R. G. F.
Characterization and localization of repetitive DNA sequences in the ornamental Alstroemeria aurea Graham
Key words Alstroemeria aurea
Repetitive DNA
FISH-fluorescence in situ hybridization
Heterochromatin
Phylogeny
Key words Alstroemeria aurea
Repetitive DNA
FISH-fluorescence in situ hybridization
Heterochromatin
Phylogeny
Abstract  Three repetitive DNA sequences were isolated from a genomic DNA library of the ornamental Alstroemeria aurea Graham. Two repeats, A001-I and A001-II, were quite homologous and highly A. aurea-specific. A001-I was a 217-bp sequence with several telomeric TTTAGGG repeats at the 5′ end and a unique sequence of 98 bp at the other end. The third repeat, A001-IV, was a 840-bp sequence which contained two sub-sequences of 56 and 74 bp respectively, previously found in chloroplast (cp) DNA of tobacco and spinach and to a lesser extent in the cpDNA of maize and rice. Repeat A001-IV was not species-specific and its hybridization signal was weaker than the other repeats. Fluorescence in situ hybridization (FISH) revealed the A. aurea-specific repeats to be located in the heterochromatic regions of all A. aurea chromosomes. The differences in FISH pattern make them useful tools for karyotype analysis. The non-species-specific sequence A001-IV gave a dispersed signal over all the Alstroemeria chromosomes in an interspecific hybrid. The potential use of these repetitive DNA sequences for the study of phylogenetic relationships within the genus Alstroemeria is discussed.
1432-2242
14322242
Springer
shingle_catch_all_3 Jeu, M. J. De
Lasschuit, J.
Kuipers, A. G. J.
Kamstra, S. A.
Visser, R. G. F.
Characterization and localization of repetitive DNA sequences in the ornamental Alstroemeria aurea Graham
Key words Alstroemeria aurea
Repetitive DNA
FISH-fluorescence in situ hybridization
Heterochromatin
Phylogeny
Key words Alstroemeria aurea
Repetitive DNA
FISH-fluorescence in situ hybridization
Heterochromatin
Phylogeny
Abstract  Three repetitive DNA sequences were isolated from a genomic DNA library of the ornamental Alstroemeria aurea Graham. Two repeats, A001-I and A001-II, were quite homologous and highly A. aurea-specific. A001-I was a 217-bp sequence with several telomeric TTTAGGG repeats at the 5′ end and a unique sequence of 98 bp at the other end. The third repeat, A001-IV, was a 840-bp sequence which contained two sub-sequences of 56 and 74 bp respectively, previously found in chloroplast (cp) DNA of tobacco and spinach and to a lesser extent in the cpDNA of maize and rice. Repeat A001-IV was not species-specific and its hybridization signal was weaker than the other repeats. Fluorescence in situ hybridization (FISH) revealed the A. aurea-specific repeats to be located in the heterochromatic regions of all A. aurea chromosomes. The differences in FISH pattern make them useful tools for karyotype analysis. The non-species-specific sequence A001-IV gave a dispersed signal over all the Alstroemeria chromosomes in an interspecific hybrid. The potential use of these repetitive DNA sequences for the study of phylogenetic relationships within the genus Alstroemeria is discussed.
1432-2242
14322242
Springer
shingle_catch_all_4 Jeu, M. J. De
Lasschuit, J.
Kuipers, A. G. J.
Kamstra, S. A.
Visser, R. G. F.
Characterization and localization of repetitive DNA sequences in the ornamental Alstroemeria aurea Graham
Key words Alstroemeria aurea
Repetitive DNA
FISH-fluorescence in situ hybridization
Heterochromatin
Phylogeny
Key words Alstroemeria aurea
Repetitive DNA
FISH-fluorescence in situ hybridization
Heterochromatin
Phylogeny
Abstract  Three repetitive DNA sequences were isolated from a genomic DNA library of the ornamental Alstroemeria aurea Graham. Two repeats, A001-I and A001-II, were quite homologous and highly A. aurea-specific. A001-I was a 217-bp sequence with several telomeric TTTAGGG repeats at the 5′ end and a unique sequence of 98 bp at the other end. The third repeat, A001-IV, was a 840-bp sequence which contained two sub-sequences of 56 and 74 bp respectively, previously found in chloroplast (cp) DNA of tobacco and spinach and to a lesser extent in the cpDNA of maize and rice. Repeat A001-IV was not species-specific and its hybridization signal was weaker than the other repeats. Fluorescence in situ hybridization (FISH) revealed the A. aurea-specific repeats to be located in the heterochromatic regions of all A. aurea chromosomes. The differences in FISH pattern make them useful tools for karyotype analysis. The non-species-specific sequence A001-IV gave a dispersed signal over all the Alstroemeria chromosomes in an interspecific hybrid. The potential use of these repetitive DNA sequences for the study of phylogenetic relationships within the genus Alstroemeria is discussed.
1432-2242
14322242
Springer
shingle_title_1 Characterization and localization of repetitive DNA sequences in the ornamental Alstroemeria aurea Graham
shingle_title_2 Characterization and localization of repetitive DNA sequences in the ornamental Alstroemeria aurea Graham
shingle_title_3 Characterization and localization of repetitive DNA sequences in the ornamental Alstroemeria aurea Graham
shingle_title_4 Characterization and localization of repetitive DNA sequences in the ornamental Alstroemeria aurea Graham
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source_archive Springer Online Journal Archives 1860-2000
timestamp 2024-05-06T09:44:40.918Z
titel Characterization and localization of repetitive DNA sequences in the ornamental Alstroemeria aurea Graham
titel_suche Characterization and localization of repetitive DNA sequences in the ornamental Alstroemeria aurea Graham
topic W
uid nat_lic_papers_NLM199880409