The first and second backcross progeny of the intergeneric fusion hybrids of potato and tomato after crossing with potato
Jacobsen, E. ; Daniel, M. K. ; Bergervoet-van Deelen, J. E. M. ; Huigen, D. J. ; Ramanna, M. S.
Springer
Published 1994
Springer
Published 1994
| ISSN: |
1432-2242
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|---|---|
| Keywords: |
Protoplast fusion ; Potato ; Tomato ; Post-fertilisation barriers ; Introgression
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| Source: |
Springer Online Journal Archives 1860-2000
|
| Topics: |
Biology
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| Notes: |
Abstract Somatic fusion hybrids between the diploid potato and tomato were backcrossed to several genotypes of potato. Two ploidy levels of fusion hybrids, 4x and 6x, were used as female parents in backcrosses with five clones of 4x-potato. An estimate of the berry set and “seed set” in immature berries harvested 14–21 days after pollination indicated that crosses between certain combinations of 6x-fusion hybrids and male parents were more successful than others. The culture of over 4000 young seeds from berries harvested 2–2.5 weeks after pollination gave rise to a single seedling, 93.6701, from the cross between the 6x-fusion hybrid C 31-17-1 and the 4x-potato AM 66.42. This seedling was found to possess a pentaploid chromosome number, which was expected of a 6x × 4x cross. Isozyme analysis and DNA hybridisation studies confirmed that the seedling 93.6701 was indeed a backcross (BC1) progeny. Morphologically, this BC1 plant resembled potato with respect to plant habit, leaf shape, stolons and tuber characteristics, while some of the characters, such as floral morphology and the fragrance of the crushed leaves (typical of tomato), were intermediate. It was male sterile but could be successfully hybridized with 4x-potato through in vitro culture of yound seeds; thus, BC2 plants were obtained. The possibilities of backcrossing and the potential use of BC1 and BC2 plants in genetics and breeding are discussed.
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| Type of Medium: |
Electronic Resource
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| URL: |
| _version_ | 1798295970050473985 |
|---|---|
| autor | Jacobsen, E. Daniel, M. K. Bergervoet-van Deelen, J. E. M. Huigen, D. J. Ramanna, M. S. |
| autorsonst | Jacobsen, E. Daniel, M. K. Bergervoet-van Deelen, J. E. M. Huigen, D. J. Ramanna, M. S. |
| book_url | http://dx.doi.org/10.1007/BF00225895 |
| datenlieferant | nat_lic_papers |
| hauptsatz | hsatz_simple |
| identnr | NLM199865655 |
| issn | 1432-2242 |
| journal_name | Theoretical and applied genetics |
| materialart | 1 |
| notes | Abstract Somatic fusion hybrids between the diploid potato and tomato were backcrossed to several genotypes of potato. Two ploidy levels of fusion hybrids, 4x and 6x, were used as female parents in backcrosses with five clones of 4x-potato. An estimate of the berry set and “seed set” in immature berries harvested 14–21 days after pollination indicated that crosses between certain combinations of 6x-fusion hybrids and male parents were more successful than others. The culture of over 4000 young seeds from berries harvested 2–2.5 weeks after pollination gave rise to a single seedling, 93.6701, from the cross between the 6x-fusion hybrid C 31-17-1 and the 4x-potato AM 66.42. This seedling was found to possess a pentaploid chromosome number, which was expected of a 6x × 4x cross. Isozyme analysis and DNA hybridisation studies confirmed that the seedling 93.6701 was indeed a backcross (BC1) progeny. Morphologically, this BC1 plant resembled potato with respect to plant habit, leaf shape, stolons and tuber characteristics, while some of the characters, such as floral morphology and the fragrance of the crushed leaves (typical of tomato), were intermediate. It was male sterile but could be successfully hybridized with 4x-potato through in vitro culture of yound seeds; thus, BC2 plants were obtained. The possibilities of backcrossing and the potential use of BC1 and BC2 plants in genetics and breeding are discussed. |
| package_name | Springer |
| publikationsjahr_anzeige | 1994 |
| publikationsjahr_facette | 1994 |
| publikationsjahr_intervall | 8009:1990-1994 |
| publikationsjahr_sort | 1994 |
| publisher | Springer |
| reference | 88 (1994), S. 181-186 |
| schlagwort | Protoplast fusion Potato Tomato Post-fertilisation barriers Introgression |
| search_space | articles |
| shingle_author_1 | Jacobsen, E. Daniel, M. K. Bergervoet-van Deelen, J. E. M. Huigen, D. J. Ramanna, M. S. |
| shingle_author_2 | Jacobsen, E. Daniel, M. K. Bergervoet-van Deelen, J. E. M. Huigen, D. J. Ramanna, M. S. |
| shingle_author_3 | Jacobsen, E. Daniel, M. K. Bergervoet-van Deelen, J. E. M. Huigen, D. J. Ramanna, M. S. |
| shingle_author_4 | Jacobsen, E. Daniel, M. K. Bergervoet-van Deelen, J. E. M. Huigen, D. J. Ramanna, M. S. |
| shingle_catch_all_1 | Jacobsen, E. Daniel, M. K. Bergervoet-van Deelen, J. E. M. Huigen, D. J. Ramanna, M. S. The first and second backcross progeny of the intergeneric fusion hybrids of potato and tomato after crossing with potato Protoplast fusion Potato Tomato Post-fertilisation barriers Introgression Protoplast fusion Potato Tomato Post-fertilisation barriers Introgression Abstract Somatic fusion hybrids between the diploid potato and tomato were backcrossed to several genotypes of potato. Two ploidy levels of fusion hybrids, 4x and 6x, were used as female parents in backcrosses with five clones of 4x-potato. An estimate of the berry set and “seed set” in immature berries harvested 14–21 days after pollination indicated that crosses between certain combinations of 6x-fusion hybrids and male parents were more successful than others. The culture of over 4000 young seeds from berries harvested 2–2.5 weeks after pollination gave rise to a single seedling, 93.6701, from the cross between the 6x-fusion hybrid C 31-17-1 and the 4x-potato AM 66.42. This seedling was found to possess a pentaploid chromosome number, which was expected of a 6x × 4x cross. Isozyme analysis and DNA hybridisation studies confirmed that the seedling 93.6701 was indeed a backcross (BC1) progeny. Morphologically, this BC1 plant resembled potato with respect to plant habit, leaf shape, stolons and tuber characteristics, while some of the characters, such as floral morphology and the fragrance of the crushed leaves (typical of tomato), were intermediate. It was male sterile but could be successfully hybridized with 4x-potato through in vitro culture of yound seeds; thus, BC2 plants were obtained. The possibilities of backcrossing and the potential use of BC1 and BC2 plants in genetics and breeding are discussed. 1432-2242 14322242 Springer |
| shingle_catch_all_2 | Jacobsen, E. Daniel, M. K. Bergervoet-van Deelen, J. E. M. Huigen, D. J. Ramanna, M. S. The first and second backcross progeny of the intergeneric fusion hybrids of potato and tomato after crossing with potato Protoplast fusion Potato Tomato Post-fertilisation barriers Introgression Protoplast fusion Potato Tomato Post-fertilisation barriers Introgression Abstract Somatic fusion hybrids between the diploid potato and tomato were backcrossed to several genotypes of potato. Two ploidy levels of fusion hybrids, 4x and 6x, were used as female parents in backcrosses with five clones of 4x-potato. An estimate of the berry set and “seed set” in immature berries harvested 14–21 days after pollination indicated that crosses between certain combinations of 6x-fusion hybrids and male parents were more successful than others. The culture of over 4000 young seeds from berries harvested 2–2.5 weeks after pollination gave rise to a single seedling, 93.6701, from the cross between the 6x-fusion hybrid C 31-17-1 and the 4x-potato AM 66.42. This seedling was found to possess a pentaploid chromosome number, which was expected of a 6x × 4x cross. Isozyme analysis and DNA hybridisation studies confirmed that the seedling 93.6701 was indeed a backcross (BC1) progeny. Morphologically, this BC1 plant resembled potato with respect to plant habit, leaf shape, stolons and tuber characteristics, while some of the characters, such as floral morphology and the fragrance of the crushed leaves (typical of tomato), were intermediate. It was male sterile but could be successfully hybridized with 4x-potato through in vitro culture of yound seeds; thus, BC2 plants were obtained. The possibilities of backcrossing and the potential use of BC1 and BC2 plants in genetics and breeding are discussed. 1432-2242 14322242 Springer |
| shingle_catch_all_3 | Jacobsen, E. Daniel, M. K. Bergervoet-van Deelen, J. E. M. Huigen, D. J. Ramanna, M. S. The first and second backcross progeny of the intergeneric fusion hybrids of potato and tomato after crossing with potato Protoplast fusion Potato Tomato Post-fertilisation barriers Introgression Protoplast fusion Potato Tomato Post-fertilisation barriers Introgression Abstract Somatic fusion hybrids between the diploid potato and tomato were backcrossed to several genotypes of potato. Two ploidy levels of fusion hybrids, 4x and 6x, were used as female parents in backcrosses with five clones of 4x-potato. An estimate of the berry set and “seed set” in immature berries harvested 14–21 days after pollination indicated that crosses between certain combinations of 6x-fusion hybrids and male parents were more successful than others. The culture of over 4000 young seeds from berries harvested 2–2.5 weeks after pollination gave rise to a single seedling, 93.6701, from the cross between the 6x-fusion hybrid C 31-17-1 and the 4x-potato AM 66.42. This seedling was found to possess a pentaploid chromosome number, which was expected of a 6x × 4x cross. Isozyme analysis and DNA hybridisation studies confirmed that the seedling 93.6701 was indeed a backcross (BC1) progeny. Morphologically, this BC1 plant resembled potato with respect to plant habit, leaf shape, stolons and tuber characteristics, while some of the characters, such as floral morphology and the fragrance of the crushed leaves (typical of tomato), were intermediate. It was male sterile but could be successfully hybridized with 4x-potato through in vitro culture of yound seeds; thus, BC2 plants were obtained. The possibilities of backcrossing and the potential use of BC1 and BC2 plants in genetics and breeding are discussed. 1432-2242 14322242 Springer |
| shingle_catch_all_4 | Jacobsen, E. Daniel, M. K. Bergervoet-van Deelen, J. E. M. Huigen, D. J. Ramanna, M. S. The first and second backcross progeny of the intergeneric fusion hybrids of potato and tomato after crossing with potato Protoplast fusion Potato Tomato Post-fertilisation barriers Introgression Protoplast fusion Potato Tomato Post-fertilisation barriers Introgression Abstract Somatic fusion hybrids between the diploid potato and tomato were backcrossed to several genotypes of potato. Two ploidy levels of fusion hybrids, 4x and 6x, were used as female parents in backcrosses with five clones of 4x-potato. An estimate of the berry set and “seed set” in immature berries harvested 14–21 days after pollination indicated that crosses between certain combinations of 6x-fusion hybrids and male parents were more successful than others. The culture of over 4000 young seeds from berries harvested 2–2.5 weeks after pollination gave rise to a single seedling, 93.6701, from the cross between the 6x-fusion hybrid C 31-17-1 and the 4x-potato AM 66.42. This seedling was found to possess a pentaploid chromosome number, which was expected of a 6x × 4x cross. Isozyme analysis and DNA hybridisation studies confirmed that the seedling 93.6701 was indeed a backcross (BC1) progeny. Morphologically, this BC1 plant resembled potato with respect to plant habit, leaf shape, stolons and tuber characteristics, while some of the characters, such as floral morphology and the fragrance of the crushed leaves (typical of tomato), were intermediate. It was male sterile but could be successfully hybridized with 4x-potato through in vitro culture of yound seeds; thus, BC2 plants were obtained. The possibilities of backcrossing and the potential use of BC1 and BC2 plants in genetics and breeding are discussed. 1432-2242 14322242 Springer |
| shingle_title_1 | The first and second backcross progeny of the intergeneric fusion hybrids of potato and tomato after crossing with potato |
| shingle_title_2 | The first and second backcross progeny of the intergeneric fusion hybrids of potato and tomato after crossing with potato |
| shingle_title_3 | The first and second backcross progeny of the intergeneric fusion hybrids of potato and tomato after crossing with potato |
| shingle_title_4 | The first and second backcross progeny of the intergeneric fusion hybrids of potato and tomato after crossing with potato |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Springer Online Journal Archives 1860-2000 |
| timestamp | 2024-05-06T09:44:38.709Z |
| titel | The first and second backcross progeny of the intergeneric fusion hybrids of potato and tomato after crossing with potato |
| titel_suche | The first and second backcross progeny of the intergeneric fusion hybrids of potato and tomato after crossing with potato |
| topic | W |
| uid | nat_lic_papers_NLM199865655 |