An integrated AFLP and RFLP Brassica oleracea linkage map from two morphologically distinct doubled-haploid mapping populations

ISSN:
1432-2242
Keywords:
Key words Brassica oleracea ; Integrated map ; Molecular markers ; Doubled-haploid ; Comparative mapping
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract  Genetical maps of molecular markers in two very different F1-derived doubled-haploid populations of Brassica oleracea are compared and the first integrated map described. The F1 crosses were: Chinese kale×calabrese (var. alboglabra×var. italica) and cauliflower×Brussels sprout (var. botrytis×var. gemmifera). Integration of the two component maps using Joinmap v.2.0 was based on 105 common loci including RFLPs, AFLPs and microsatellites. This provided an effective method of producing a high-density consensus linkage map of the B. oleracea genome. Based on 547 markers mapping to nine linkage groups, the integrated map covers a total map length of 893 cM, with an average locus interval of 2.6 cM. Comparisons back to the component linkage maps revealed similar sequences of common markers, although significant differences in recombination frequency were observed between some pairs of homologous markers. Map integration resulted in an increased locus density and effective population size, providing a stronger framework for subsequent physical mapping and for precision mapping of QTLs using substitution lines.
Type of Medium:
Electronic Resource
URL:
_version_ 1798295962362314752
autor Sebastian, R. L.
Howell, E. C.
King, G. J.
Marshall, D. F.
Kearsey, M. J.
autorsonst Sebastian, R. L.
Howell, E. C.
King, G. J.
Marshall, D. F.
Kearsey, M. J.
book_url http://dx.doi.org/10.1007/s001220050011
datenlieferant nat_lic_papers
hauptsatz hsatz_simple
identnr NLM199795835
issn 1432-2242
journal_name Theoretical and applied genetics
materialart 1
notes Abstract  Genetical maps of molecular markers in two very different F1-derived doubled-haploid populations of Brassica oleracea are compared and the first integrated map described. The F1 crosses were: Chinese kale×calabrese (var. alboglabra×var. italica) and cauliflower×Brussels sprout (var. botrytis×var. gemmifera). Integration of the two component maps using Joinmap v.2.0 was based on 105 common loci including RFLPs, AFLPs and microsatellites. This provided an effective method of producing a high-density consensus linkage map of the B. oleracea genome. Based on 547 markers mapping to nine linkage groups, the integrated map covers a total map length of 893 cM, with an average locus interval of 2.6 cM. Comparisons back to the component linkage maps revealed similar sequences of common markers, although significant differences in recombination frequency were observed between some pairs of homologous markers. Map integration resulted in an increased locus density and effective population size, providing a stronger framework for subsequent physical mapping and for precision mapping of QTLs using substitution lines.
package_name Springer
publikationsjahr_anzeige 2000
publikationsjahr_facette 2000
publikationsjahr_intervall 7999:2000-2004
publikationsjahr_sort 2000
publisher Springer
reference 100 (2000), S. 75-81
schlagwort Key words Brassica oleracea
Integrated map
Molecular markers
Doubled-haploid
Comparative mapping
search_space articles
shingle_author_1 Sebastian, R. L.
Howell, E. C.
King, G. J.
Marshall, D. F.
Kearsey, M. J.
shingle_author_2 Sebastian, R. L.
Howell, E. C.
King, G. J.
Marshall, D. F.
Kearsey, M. J.
shingle_author_3 Sebastian, R. L.
Howell, E. C.
King, G. J.
Marshall, D. F.
Kearsey, M. J.
shingle_author_4 Sebastian, R. L.
Howell, E. C.
King, G. J.
Marshall, D. F.
Kearsey, M. J.
shingle_catch_all_1 Sebastian, R. L.
Howell, E. C.
King, G. J.
Marshall, D. F.
Kearsey, M. J.
An integrated AFLP and RFLP Brassica oleracea linkage map from two morphologically distinct doubled-haploid mapping populations
Key words Brassica oleracea
Integrated map
Molecular markers
Doubled-haploid
Comparative mapping
Key words Brassica oleracea
Integrated map
Molecular markers
Doubled-haploid
Comparative mapping
Abstract  Genetical maps of molecular markers in two very different F1-derived doubled-haploid populations of Brassica oleracea are compared and the first integrated map described. The F1 crosses were: Chinese kale×calabrese (var. alboglabra×var. italica) and cauliflower×Brussels sprout (var. botrytis×var. gemmifera). Integration of the two component maps using Joinmap v.2.0 was based on 105 common loci including RFLPs, AFLPs and microsatellites. This provided an effective method of producing a high-density consensus linkage map of the B. oleracea genome. Based on 547 markers mapping to nine linkage groups, the integrated map covers a total map length of 893 cM, with an average locus interval of 2.6 cM. Comparisons back to the component linkage maps revealed similar sequences of common markers, although significant differences in recombination frequency were observed between some pairs of homologous markers. Map integration resulted in an increased locus density and effective population size, providing a stronger framework for subsequent physical mapping and for precision mapping of QTLs using substitution lines.
1432-2242
14322242
Springer
shingle_catch_all_2 Sebastian, R. L.
Howell, E. C.
King, G. J.
Marshall, D. F.
Kearsey, M. J.
An integrated AFLP and RFLP Brassica oleracea linkage map from two morphologically distinct doubled-haploid mapping populations
Key words Brassica oleracea
Integrated map
Molecular markers
Doubled-haploid
Comparative mapping
Key words Brassica oleracea
Integrated map
Molecular markers
Doubled-haploid
Comparative mapping
Abstract  Genetical maps of molecular markers in two very different F1-derived doubled-haploid populations of Brassica oleracea are compared and the first integrated map described. The F1 crosses were: Chinese kale×calabrese (var. alboglabra×var. italica) and cauliflower×Brussels sprout (var. botrytis×var. gemmifera). Integration of the two component maps using Joinmap v.2.0 was based on 105 common loci including RFLPs, AFLPs and microsatellites. This provided an effective method of producing a high-density consensus linkage map of the B. oleracea genome. Based on 547 markers mapping to nine linkage groups, the integrated map covers a total map length of 893 cM, with an average locus interval of 2.6 cM. Comparisons back to the component linkage maps revealed similar sequences of common markers, although significant differences in recombination frequency were observed between some pairs of homologous markers. Map integration resulted in an increased locus density and effective population size, providing a stronger framework for subsequent physical mapping and for precision mapping of QTLs using substitution lines.
1432-2242
14322242
Springer
shingle_catch_all_3 Sebastian, R. L.
Howell, E. C.
King, G. J.
Marshall, D. F.
Kearsey, M. J.
An integrated AFLP and RFLP Brassica oleracea linkage map from two morphologically distinct doubled-haploid mapping populations
Key words Brassica oleracea
Integrated map
Molecular markers
Doubled-haploid
Comparative mapping
Key words Brassica oleracea
Integrated map
Molecular markers
Doubled-haploid
Comparative mapping
Abstract  Genetical maps of molecular markers in two very different F1-derived doubled-haploid populations of Brassica oleracea are compared and the first integrated map described. The F1 crosses were: Chinese kale×calabrese (var. alboglabra×var. italica) and cauliflower×Brussels sprout (var. botrytis×var. gemmifera). Integration of the two component maps using Joinmap v.2.0 was based on 105 common loci including RFLPs, AFLPs and microsatellites. This provided an effective method of producing a high-density consensus linkage map of the B. oleracea genome. Based on 547 markers mapping to nine linkage groups, the integrated map covers a total map length of 893 cM, with an average locus interval of 2.6 cM. Comparisons back to the component linkage maps revealed similar sequences of common markers, although significant differences in recombination frequency were observed between some pairs of homologous markers. Map integration resulted in an increased locus density and effective population size, providing a stronger framework for subsequent physical mapping and for precision mapping of QTLs using substitution lines.
1432-2242
14322242
Springer
shingle_catch_all_4 Sebastian, R. L.
Howell, E. C.
King, G. J.
Marshall, D. F.
Kearsey, M. J.
An integrated AFLP and RFLP Brassica oleracea linkage map from two morphologically distinct doubled-haploid mapping populations
Key words Brassica oleracea
Integrated map
Molecular markers
Doubled-haploid
Comparative mapping
Key words Brassica oleracea
Integrated map
Molecular markers
Doubled-haploid
Comparative mapping
Abstract  Genetical maps of molecular markers in two very different F1-derived doubled-haploid populations of Brassica oleracea are compared and the first integrated map described. The F1 crosses were: Chinese kale×calabrese (var. alboglabra×var. italica) and cauliflower×Brussels sprout (var. botrytis×var. gemmifera). Integration of the two component maps using Joinmap v.2.0 was based on 105 common loci including RFLPs, AFLPs and microsatellites. This provided an effective method of producing a high-density consensus linkage map of the B. oleracea genome. Based on 547 markers mapping to nine linkage groups, the integrated map covers a total map length of 893 cM, with an average locus interval of 2.6 cM. Comparisons back to the component linkage maps revealed similar sequences of common markers, although significant differences in recombination frequency were observed between some pairs of homologous markers. Map integration resulted in an increased locus density and effective population size, providing a stronger framework for subsequent physical mapping and for precision mapping of QTLs using substitution lines.
1432-2242
14322242
Springer
shingle_title_1 An integrated AFLP and RFLP Brassica oleracea linkage map from two morphologically distinct doubled-haploid mapping populations
shingle_title_2 An integrated AFLP and RFLP Brassica oleracea linkage map from two morphologically distinct doubled-haploid mapping populations
shingle_title_3 An integrated AFLP and RFLP Brassica oleracea linkage map from two morphologically distinct doubled-haploid mapping populations
shingle_title_4 An integrated AFLP and RFLP Brassica oleracea linkage map from two morphologically distinct doubled-haploid mapping populations
sigel_instance_filter dkfz
geomar
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source_archive Springer Online Journal Archives 1860-2000
timestamp 2024-05-06T09:44:32.476Z
titel An integrated AFLP and RFLP Brassica oleracea linkage map from two morphologically distinct doubled-haploid mapping populations
titel_suche An integrated AFLP and RFLP Brassica oleracea linkage map from two morphologically distinct doubled-haploid mapping populations
topic W
uid nat_lic_papers_NLM199795835