An integrated AFLP and RFLP Brassica oleracea linkage map from two morphologically distinct doubled-haploid mapping populations
Sebastian, R. L. ; Howell, E. C. ; King, G. J. ; Marshall, D. F. ; Kearsey, M. J.
Springer
Published 2000
Springer
Published 2000
ISSN: |
1432-2242
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Keywords: |
Key words Brassica oleracea ; Integrated map ; Molecular markers ; Doubled-haploid ; Comparative mapping
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Source: |
Springer Online Journal Archives 1860-2000
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Topics: |
Biology
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Notes: |
Abstract Genetical maps of molecular markers in two very different F1-derived doubled-haploid populations of Brassica oleracea are compared and the first integrated map described. The F1 crosses were: Chinese kale×calabrese (var. alboglabra×var. italica) and cauliflower×Brussels sprout (var. botrytis×var. gemmifera). Integration of the two component maps using Joinmap v.2.0 was based on 105 common loci including RFLPs, AFLPs and microsatellites. This provided an effective method of producing a high-density consensus linkage map of the B. oleracea genome. Based on 547 markers mapping to nine linkage groups, the integrated map covers a total map length of 893 cM, with an average locus interval of 2.6 cM. Comparisons back to the component linkage maps revealed similar sequences of common markers, although significant differences in recombination frequency were observed between some pairs of homologous markers. Map integration resulted in an increased locus density and effective population size, providing a stronger framework for subsequent physical mapping and for precision mapping of QTLs using substitution lines.
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Type of Medium: |
Electronic Resource
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URL: |
_version_ | 1798295962362314752 |
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autor | Sebastian, R. L. Howell, E. C. King, G. J. Marshall, D. F. Kearsey, M. J. |
autorsonst | Sebastian, R. L. Howell, E. C. King, G. J. Marshall, D. F. Kearsey, M. J. |
book_url | http://dx.doi.org/10.1007/s001220050011 |
datenlieferant | nat_lic_papers |
hauptsatz | hsatz_simple |
identnr | NLM199795835 |
issn | 1432-2242 |
journal_name | Theoretical and applied genetics |
materialart | 1 |
notes | Abstract Genetical maps of molecular markers in two very different F1-derived doubled-haploid populations of Brassica oleracea are compared and the first integrated map described. The F1 crosses were: Chinese kale×calabrese (var. alboglabra×var. italica) and cauliflower×Brussels sprout (var. botrytis×var. gemmifera). Integration of the two component maps using Joinmap v.2.0 was based on 105 common loci including RFLPs, AFLPs and microsatellites. This provided an effective method of producing a high-density consensus linkage map of the B. oleracea genome. Based on 547 markers mapping to nine linkage groups, the integrated map covers a total map length of 893 cM, with an average locus interval of 2.6 cM. Comparisons back to the component linkage maps revealed similar sequences of common markers, although significant differences in recombination frequency were observed between some pairs of homologous markers. Map integration resulted in an increased locus density and effective population size, providing a stronger framework for subsequent physical mapping and for precision mapping of QTLs using substitution lines. |
package_name | Springer |
publikationsjahr_anzeige | 2000 |
publikationsjahr_facette | 2000 |
publikationsjahr_intervall | 7999:2000-2004 |
publikationsjahr_sort | 2000 |
publisher | Springer |
reference | 100 (2000), S. 75-81 |
schlagwort | Key words Brassica oleracea Integrated map Molecular markers Doubled-haploid Comparative mapping |
search_space | articles |
shingle_author_1 | Sebastian, R. L. Howell, E. C. King, G. J. Marshall, D. F. Kearsey, M. J. |
shingle_author_2 | Sebastian, R. L. Howell, E. C. King, G. J. Marshall, D. F. Kearsey, M. J. |
shingle_author_3 | Sebastian, R. L. Howell, E. C. King, G. J. Marshall, D. F. Kearsey, M. J. |
shingle_author_4 | Sebastian, R. L. Howell, E. C. King, G. J. Marshall, D. F. Kearsey, M. J. |
shingle_catch_all_1 | Sebastian, R. L. Howell, E. C. King, G. J. Marshall, D. F. Kearsey, M. J. An integrated AFLP and RFLP Brassica oleracea linkage map from two morphologically distinct doubled-haploid mapping populations Key words Brassica oleracea Integrated map Molecular markers Doubled-haploid Comparative mapping Key words Brassica oleracea Integrated map Molecular markers Doubled-haploid Comparative mapping Abstract Genetical maps of molecular markers in two very different F1-derived doubled-haploid populations of Brassica oleracea are compared and the first integrated map described. The F1 crosses were: Chinese kale×calabrese (var. alboglabra×var. italica) and cauliflower×Brussels sprout (var. botrytis×var. gemmifera). Integration of the two component maps using Joinmap v.2.0 was based on 105 common loci including RFLPs, AFLPs and microsatellites. This provided an effective method of producing a high-density consensus linkage map of the B. oleracea genome. Based on 547 markers mapping to nine linkage groups, the integrated map covers a total map length of 893 cM, with an average locus interval of 2.6 cM. Comparisons back to the component linkage maps revealed similar sequences of common markers, although significant differences in recombination frequency were observed between some pairs of homologous markers. Map integration resulted in an increased locus density and effective population size, providing a stronger framework for subsequent physical mapping and for precision mapping of QTLs using substitution lines. 1432-2242 14322242 Springer |
shingle_catch_all_2 | Sebastian, R. L. Howell, E. C. King, G. J. Marshall, D. F. Kearsey, M. J. An integrated AFLP and RFLP Brassica oleracea linkage map from two morphologically distinct doubled-haploid mapping populations Key words Brassica oleracea Integrated map Molecular markers Doubled-haploid Comparative mapping Key words Brassica oleracea Integrated map Molecular markers Doubled-haploid Comparative mapping Abstract Genetical maps of molecular markers in two very different F1-derived doubled-haploid populations of Brassica oleracea are compared and the first integrated map described. The F1 crosses were: Chinese kale×calabrese (var. alboglabra×var. italica) and cauliflower×Brussels sprout (var. botrytis×var. gemmifera). Integration of the two component maps using Joinmap v.2.0 was based on 105 common loci including RFLPs, AFLPs and microsatellites. This provided an effective method of producing a high-density consensus linkage map of the B. oleracea genome. Based on 547 markers mapping to nine linkage groups, the integrated map covers a total map length of 893 cM, with an average locus interval of 2.6 cM. Comparisons back to the component linkage maps revealed similar sequences of common markers, although significant differences in recombination frequency were observed between some pairs of homologous markers. Map integration resulted in an increased locus density and effective population size, providing a stronger framework for subsequent physical mapping and for precision mapping of QTLs using substitution lines. 1432-2242 14322242 Springer |
shingle_catch_all_3 | Sebastian, R. L. Howell, E. C. King, G. J. Marshall, D. F. Kearsey, M. J. An integrated AFLP and RFLP Brassica oleracea linkage map from two morphologically distinct doubled-haploid mapping populations Key words Brassica oleracea Integrated map Molecular markers Doubled-haploid Comparative mapping Key words Brassica oleracea Integrated map Molecular markers Doubled-haploid Comparative mapping Abstract Genetical maps of molecular markers in two very different F1-derived doubled-haploid populations of Brassica oleracea are compared and the first integrated map described. The F1 crosses were: Chinese kale×calabrese (var. alboglabra×var. italica) and cauliflower×Brussels sprout (var. botrytis×var. gemmifera). Integration of the two component maps using Joinmap v.2.0 was based on 105 common loci including RFLPs, AFLPs and microsatellites. This provided an effective method of producing a high-density consensus linkage map of the B. oleracea genome. Based on 547 markers mapping to nine linkage groups, the integrated map covers a total map length of 893 cM, with an average locus interval of 2.6 cM. Comparisons back to the component linkage maps revealed similar sequences of common markers, although significant differences in recombination frequency were observed between some pairs of homologous markers. Map integration resulted in an increased locus density and effective population size, providing a stronger framework for subsequent physical mapping and for precision mapping of QTLs using substitution lines. 1432-2242 14322242 Springer |
shingle_catch_all_4 | Sebastian, R. L. Howell, E. C. King, G. J. Marshall, D. F. Kearsey, M. J. An integrated AFLP and RFLP Brassica oleracea linkage map from two morphologically distinct doubled-haploid mapping populations Key words Brassica oleracea Integrated map Molecular markers Doubled-haploid Comparative mapping Key words Brassica oleracea Integrated map Molecular markers Doubled-haploid Comparative mapping Abstract Genetical maps of molecular markers in two very different F1-derived doubled-haploid populations of Brassica oleracea are compared and the first integrated map described. The F1 crosses were: Chinese kale×calabrese (var. alboglabra×var. italica) and cauliflower×Brussels sprout (var. botrytis×var. gemmifera). Integration of the two component maps using Joinmap v.2.0 was based on 105 common loci including RFLPs, AFLPs and microsatellites. This provided an effective method of producing a high-density consensus linkage map of the B. oleracea genome. Based on 547 markers mapping to nine linkage groups, the integrated map covers a total map length of 893 cM, with an average locus interval of 2.6 cM. Comparisons back to the component linkage maps revealed similar sequences of common markers, although significant differences in recombination frequency were observed between some pairs of homologous markers. Map integration resulted in an increased locus density and effective population size, providing a stronger framework for subsequent physical mapping and for precision mapping of QTLs using substitution lines. 1432-2242 14322242 Springer |
shingle_title_1 | An integrated AFLP and RFLP Brassica oleracea linkage map from two morphologically distinct doubled-haploid mapping populations |
shingle_title_2 | An integrated AFLP and RFLP Brassica oleracea linkage map from two morphologically distinct doubled-haploid mapping populations |
shingle_title_3 | An integrated AFLP and RFLP Brassica oleracea linkage map from two morphologically distinct doubled-haploid mapping populations |
shingle_title_4 | An integrated AFLP and RFLP Brassica oleracea linkage map from two morphologically distinct doubled-haploid mapping populations |
sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
source_archive | Springer Online Journal Archives 1860-2000 |
timestamp | 2024-05-06T09:44:32.476Z |
titel | An integrated AFLP and RFLP Brassica oleracea linkage map from two morphologically distinct doubled-haploid mapping populations |
titel_suche | An integrated AFLP and RFLP Brassica oleracea linkage map from two morphologically distinct doubled-haploid mapping populations |
topic | W |
uid | nat_lic_papers_NLM199795835 |