Analysis of hybridoma mutants defective in synthesis of immunoglobulin M
Trimble, W. S. ; Baker, M. D. ; Boulianne, G. L. ; Murialdo, H. ; Hozumi, N. ; Shulman, M. J.
Springer
Published 1986
Springer
Published 1986
ISSN: |
1572-9931
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Source: |
Springer Online Journal Archives 1860-2000
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Topics: |
Biology
Medicine
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Notes: |
Abstract Hybridoma mutants defective in the expression of IgM have been analyzed by molecular and somatic cell hybridization techniques. The frequency of κ light-chain mutants in the hybridoma PC7 was much higher than for other cell lines. In contrast to the mutations which we observed previously, the κ mutants examined here resulted from complete or partial deletion of the κ gene. Mutants defective in μ chain synthesis were of more diverse types including deletions, gross rearrangements, and more subtle changes. One mutant containing acis -acting mutation resulting in reduced expression of the μ chain had an associated partial duplication of the μ gene, while others making low or undetectable levels of μ had no gross alterations in genetic structure. The usefulness of this approach to the study of gene structure and expression is discussed.
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Type of Medium: |
Electronic Resource
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URL: |