Gene mapping and physical arrangements of human chromatin in transformed, hybrid cells: Fluorescent and autoradiographic in situ hybridization compared
Mitchell, A. R. ; Ambros, P. ; Gosden, J. R. ; Morten, J. E. N. ; Porteous, D. J.
Springer
Published 1986
Springer
Published 1986
| ISSN: |
1572-9931
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|---|---|
| Source: |
Springer Online Journal Archives 1860-2000
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| Topics: |
Biology
Medicine
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| Notes: |
Abstract We compare a fluorescent in situ hybridization technique, usingN-acetoxy-2-acetylaminofluorene (N-ACO-AAF) modified DNA adducts, with3H-labeled DNA in situ hybridization for (1) visualizing human transgenomes in HRAS1-selected, chromosome-mediated gene transfer (CMGT), and (2) mapping chromosomal SV40 in an SV40-transformed, human-mouse hybrid cell line. We demonstrate that individual HRAS1-CMGTs may contain multiple fragments of human chromatin. We deduce that the CMGT process can involve interstitial loss of mouse chromatin. We conclude that the N-ACO-AAF technique gives finer resolution than3H-labeled in situ hybridization. However,3H-labeling is more sensitive and has allowed us to sublocalize SV40 in Cl21 to the region 7q31–35.
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| Type of Medium: |
Electronic Resource
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| URL: |