Excitatory Amino Acid Receptors Coupled to the Phosphoinositide Pathway in Bergmann Glia
| ISSN: |
1573-6903
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|---|---|
| Keywords: |
Inositol phosphates ; radial glia ; cerebellum ; glutamate receptors
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| Source: |
Springer Online Journal Archives 1860-2000
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| Topics: |
Medicine
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| Notes: |
Abstract Glutamate (L-glu) receptors coupled to phosphoinositide hydrolysis in primary cultures of Bergmann cells from chick cerebellum were characterized biochemically and pharmacologically. Both ionotropic and metabotropic receptor agonists stimulated [3H] inositol phosphates accumulation in the following order of potency: QA〉NMDA〉L-glu〉KA∼QA〉AMPA〉〉t-ACPD. QA showed a biphasic dose-response curve (EC50 = 0.07 and 53 μM), suggesting interaction with two populations of receptors; L-glu was the most efficient agonist. Stimulation by NMDA was blocked by CPP, APS and MK-801; that by AMP A and KA was inhibited 100% by CNQX and DNQX, whereas the effect of QA was decreased both by CNQX and the metabotropic antagonist 4-CPG. Stimulation of PIP2 hydrolysis induced by metabotropic L-glu receptor agonist t-ACPD was blocked by 4-CPG but was only moderately inhibited by MCPG. EAA-induced [3H]IPs accumulation was dependent on external Ca2+ and was not affected by nifedipine verapamil, or dantrolene; thapsigargin increased the effect. Results suggest that EAA activate the PI pathway in Bergmann glia through ionotropic (NMDA and AMPA/KA) as well as metabotropic receptor subtypes (t-ACPD) which could act jointly influencing neurotransmission at the parallel fiber—Purkinje cell synapses in the cerebellum.
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| Type of Medium: |
Electronic Resource
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| URL: |
| _version_ | 1798296880274210816 |
|---|---|
| autor | López-Colomé, A. M. Ortega, A. Fragoso, G. Trueba, E. |
| autorsonst | López-Colomé, A. M. Ortega, A. Fragoso, G. Trueba, E. |
| book_url | http://dx.doi.org/10.1023/A:1022494923084 |
| datenlieferant | nat_lic_papers |
| hauptsatz | hsatz_simple |
| identnr | NLM198245432 |
| issn | 1573-6903 |
| journal_name | Neurochemical research |
| materialart | 1 |
| notes | Abstract Glutamate (L-glu) receptors coupled to phosphoinositide hydrolysis in primary cultures of Bergmann cells from chick cerebellum were characterized biochemically and pharmacologically. Both ionotropic and metabotropic receptor agonists stimulated [3H] inositol phosphates accumulation in the following order of potency: QA〉NMDA〉L-glu〉KA∼QA〉AMPA〉〉t-ACPD. QA showed a biphasic dose-response curve (EC50 = 0.07 and 53 μM), suggesting interaction with two populations of receptors; L-glu was the most efficient agonist. Stimulation by NMDA was blocked by CPP, APS and MK-801; that by AMP A and KA was inhibited 100% by CNQX and DNQX, whereas the effect of QA was decreased both by CNQX and the metabotropic antagonist 4-CPG. Stimulation of PIP2 hydrolysis induced by metabotropic L-glu receptor agonist t-ACPD was blocked by 4-CPG but was only moderately inhibited by MCPG. EAA-induced [3H]IPs accumulation was dependent on external Ca2+ and was not affected by nifedipine verapamil, or dantrolene; thapsigargin increased the effect. Results suggest that EAA activate the PI pathway in Bergmann glia through ionotropic (NMDA and AMPA/KA) as well as metabotropic receptor subtypes (t-ACPD) which could act jointly influencing neurotransmission at the parallel fiber—Purkinje cell synapses in the cerebellum. |
| package_name | Springer |
| publikationsjahr_anzeige | 1997 |
| publikationsjahr_facette | 1997 |
| publikationsjahr_intervall | 8004:1995-1999 |
| publikationsjahr_sort | 1997 |
| publisher | Springer |
| reference | 22 (1997), S. 305-312 |
| schlagwort | Inositol phosphates radial glia cerebellum glutamate receptors |
| search_space | articles |
| shingle_author_1 | López-Colomé, A. M. Ortega, A. Fragoso, G. Trueba, E. |
| shingle_author_2 | López-Colomé, A. M. Ortega, A. Fragoso, G. Trueba, E. |
| shingle_author_3 | López-Colomé, A. M. Ortega, A. Fragoso, G. Trueba, E. |
| shingle_author_4 | López-Colomé, A. M. Ortega, A. Fragoso, G. Trueba, E. |
| shingle_catch_all_1 | López-Colomé, A. M. Ortega, A. Fragoso, G. Trueba, E. Excitatory Amino Acid Receptors Coupled to the Phosphoinositide Pathway in Bergmann Glia Inositol phosphates radial glia cerebellum glutamate receptors Inositol phosphates radial glia cerebellum glutamate receptors Abstract Glutamate (L-glu) receptors coupled to phosphoinositide hydrolysis in primary cultures of Bergmann cells from chick cerebellum were characterized biochemically and pharmacologically. Both ionotropic and metabotropic receptor agonists stimulated [3H] inositol phosphates accumulation in the following order of potency: QA〉NMDA〉L-glu〉KA∼QA〉AMPA〉〉t-ACPD. QA showed a biphasic dose-response curve (EC50 = 0.07 and 53 μM), suggesting interaction with two populations of receptors; L-glu was the most efficient agonist. Stimulation by NMDA was blocked by CPP, APS and MK-801; that by AMP A and KA was inhibited 100% by CNQX and DNQX, whereas the effect of QA was decreased both by CNQX and the metabotropic antagonist 4-CPG. Stimulation of PIP2 hydrolysis induced by metabotropic L-glu receptor agonist t-ACPD was blocked by 4-CPG but was only moderately inhibited by MCPG. EAA-induced [3H]IPs accumulation was dependent on external Ca2+ and was not affected by nifedipine verapamil, or dantrolene; thapsigargin increased the effect. Results suggest that EAA activate the PI pathway in Bergmann glia through ionotropic (NMDA and AMPA/KA) as well as metabotropic receptor subtypes (t-ACPD) which could act jointly influencing neurotransmission at the parallel fiber—Purkinje cell synapses in the cerebellum. 1573-6903 15736903 Springer |
| shingle_catch_all_2 | López-Colomé, A. M. Ortega, A. Fragoso, G. Trueba, E. Excitatory Amino Acid Receptors Coupled to the Phosphoinositide Pathway in Bergmann Glia Inositol phosphates radial glia cerebellum glutamate receptors Inositol phosphates radial glia cerebellum glutamate receptors Abstract Glutamate (L-glu) receptors coupled to phosphoinositide hydrolysis in primary cultures of Bergmann cells from chick cerebellum were characterized biochemically and pharmacologically. Both ionotropic and metabotropic receptor agonists stimulated [3H] inositol phosphates accumulation in the following order of potency: QA〉NMDA〉L-glu〉KA∼QA〉AMPA〉〉t-ACPD. QA showed a biphasic dose-response curve (EC50 = 0.07 and 53 μM), suggesting interaction with two populations of receptors; L-glu was the most efficient agonist. Stimulation by NMDA was blocked by CPP, APS and MK-801; that by AMP A and KA was inhibited 100% by CNQX and DNQX, whereas the effect of QA was decreased both by CNQX and the metabotropic antagonist 4-CPG. Stimulation of PIP2 hydrolysis induced by metabotropic L-glu receptor agonist t-ACPD was blocked by 4-CPG but was only moderately inhibited by MCPG. EAA-induced [3H]IPs accumulation was dependent on external Ca2+ and was not affected by nifedipine verapamil, or dantrolene; thapsigargin increased the effect. Results suggest that EAA activate the PI pathway in Bergmann glia through ionotropic (NMDA and AMPA/KA) as well as metabotropic receptor subtypes (t-ACPD) which could act jointly influencing neurotransmission at the parallel fiber—Purkinje cell synapses in the cerebellum. 1573-6903 15736903 Springer |
| shingle_catch_all_3 | López-Colomé, A. M. Ortega, A. Fragoso, G. Trueba, E. Excitatory Amino Acid Receptors Coupled to the Phosphoinositide Pathway in Bergmann Glia Inositol phosphates radial glia cerebellum glutamate receptors Inositol phosphates radial glia cerebellum glutamate receptors Abstract Glutamate (L-glu) receptors coupled to phosphoinositide hydrolysis in primary cultures of Bergmann cells from chick cerebellum were characterized biochemically and pharmacologically. Both ionotropic and metabotropic receptor agonists stimulated [3H] inositol phosphates accumulation in the following order of potency: QA〉NMDA〉L-glu〉KA∼QA〉AMPA〉〉t-ACPD. QA showed a biphasic dose-response curve (EC50 = 0.07 and 53 μM), suggesting interaction with two populations of receptors; L-glu was the most efficient agonist. Stimulation by NMDA was blocked by CPP, APS and MK-801; that by AMP A and KA was inhibited 100% by CNQX and DNQX, whereas the effect of QA was decreased both by CNQX and the metabotropic antagonist 4-CPG. Stimulation of PIP2 hydrolysis induced by metabotropic L-glu receptor agonist t-ACPD was blocked by 4-CPG but was only moderately inhibited by MCPG. EAA-induced [3H]IPs accumulation was dependent on external Ca2+ and was not affected by nifedipine verapamil, or dantrolene; thapsigargin increased the effect. Results suggest that EAA activate the PI pathway in Bergmann glia through ionotropic (NMDA and AMPA/KA) as well as metabotropic receptor subtypes (t-ACPD) which could act jointly influencing neurotransmission at the parallel fiber—Purkinje cell synapses in the cerebellum. 1573-6903 15736903 Springer |
| shingle_catch_all_4 | López-Colomé, A. M. Ortega, A. Fragoso, G. Trueba, E. Excitatory Amino Acid Receptors Coupled to the Phosphoinositide Pathway in Bergmann Glia Inositol phosphates radial glia cerebellum glutamate receptors Inositol phosphates radial glia cerebellum glutamate receptors Abstract Glutamate (L-glu) receptors coupled to phosphoinositide hydrolysis in primary cultures of Bergmann cells from chick cerebellum were characterized biochemically and pharmacologically. Both ionotropic and metabotropic receptor agonists stimulated [3H] inositol phosphates accumulation in the following order of potency: QA〉NMDA〉L-glu〉KA∼QA〉AMPA〉〉t-ACPD. QA showed a biphasic dose-response curve (EC50 = 0.07 and 53 μM), suggesting interaction with two populations of receptors; L-glu was the most efficient agonist. Stimulation by NMDA was blocked by CPP, APS and MK-801; that by AMP A and KA was inhibited 100% by CNQX and DNQX, whereas the effect of QA was decreased both by CNQX and the metabotropic antagonist 4-CPG. Stimulation of PIP2 hydrolysis induced by metabotropic L-glu receptor agonist t-ACPD was blocked by 4-CPG but was only moderately inhibited by MCPG. EAA-induced [3H]IPs accumulation was dependent on external Ca2+ and was not affected by nifedipine verapamil, or dantrolene; thapsigargin increased the effect. Results suggest that EAA activate the PI pathway in Bergmann glia through ionotropic (NMDA and AMPA/KA) as well as metabotropic receptor subtypes (t-ACPD) which could act jointly influencing neurotransmission at the parallel fiber—Purkinje cell synapses in the cerebellum. 1573-6903 15736903 Springer |
| shingle_title_1 | Excitatory Amino Acid Receptors Coupled to the Phosphoinositide Pathway in Bergmann Glia |
| shingle_title_2 | Excitatory Amino Acid Receptors Coupled to the Phosphoinositide Pathway in Bergmann Glia |
| shingle_title_3 | Excitatory Amino Acid Receptors Coupled to the Phosphoinositide Pathway in Bergmann Glia |
| shingle_title_4 | Excitatory Amino Acid Receptors Coupled to the Phosphoinositide Pathway in Bergmann Glia |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Springer Online Journal Archives 1860-2000 |
| timestamp | 2024-05-06T09:59:07.731Z |
| titel | Excitatory Amino Acid Receptors Coupled to the Phosphoinositide Pathway in Bergmann Glia |
| titel_suche | Excitatory Amino Acid Receptors Coupled to the Phosphoinositide Pathway in Bergmann Glia |
| topic | WW-YZ |
| uid | nat_lic_papers_NLM198245432 |