Glutamine B16 insulin: Reduced insulin-like metabolic activity with moderately preserved mitogenic activity
Schwartz, Gerald P. ; Wong, Diana ; Burke, G. Thompson ; Vroede, Monique A. ; Rechler, Matthew M. ; Katsoyannis, Panayotis G.
Springer
Published 1985
Springer
Published 1985
| ISSN: |
1573-4943
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|---|---|
| Keywords: |
insulin analogue ; insulin-like growth factor ; metabolic assay ; mitogenic assay ; synthesis
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| Source: |
Springer Online Journal Archives 1860-2000
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| Topics: |
Chemistry and Pharmacology
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| Notes: |
Abstract An analogue of insulin in which the naturally occurring tyrosine residue in position B16 is replaced by a glutamine residue has been synthesized. Glutamine appears in the corresponding position in the B-domain of the insulin-like growth factors. This analogue displays 9% of the potency of insulin in binding to the insulin receptor from rat liver plasma membranes, 17% in stimulating the conversion of [3-3H] glucose into lipids in rat adipocytes, and 23% in insulin radioimmunoassay, but 40% of the potency of insulin in stimulating DNA synthesis in cultured chick fibroblasts. The analogue is a more potent mitogen than is a hybrid molecule which contains the A-chain of insulin and the entire B-domain sequence of IGF-I.
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| Type of Medium: |
Electronic Resource
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| URL: |