Embryotoxicity of micropore filters used in liquid sterilization

ISSN:
1573-7330
Keywords:
filter sterilization ; culture media ; embryotoxicity
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Microfiltration is the usual method of sterilization of tissue culture media. The filtrates of eight commercial filters were tested for embryotoxicity using mouse embryo growth. Four of five disk filters tested showed embryotoxicity in the first 5 ml of filtrate and the three largercapacity filters showed embryotoxicity for up to the first 10 ml of filtrate. The ethylene oxide-sterilized filters appeared to give poorer results than the gamma-irradiated filters. The importance of discarding the initial filtrate or preflushing such filters before use is emphasized.
Type of Medium:
Electronic Resource
URL:
_version_ 1798296904510996481
autor Harrison, K. L.
Sherrin, D. A.
Hawthorne, T. A.
Breen, T. M.
West, G. A.
Wilson, L. M.
autorsonst Harrison, K. L.
Sherrin, D. A.
Hawthorne, T. A.
Breen, T. M.
West, G. A.
Wilson, L. M.
book_url http://dx.doi.org/10.1007/BF01130588
datenlieferant nat_lic_papers
hauptsatz hsatz_simple
identnr NLM197497195
issn 1573-7330
journal_name Journal of assisted reproduction and genetics
materialart 1
notes Abstract Microfiltration is the usual method of sterilization of tissue culture media. The filtrates of eight commercial filters were tested for embryotoxicity using mouse embryo growth. Four of five disk filters tested showed embryotoxicity in the first 5 ml of filtrate and the three largercapacity filters showed embryotoxicity for up to the first 10 ml of filtrate. The ethylene oxide-sterilized filters appeared to give poorer results than the gamma-irradiated filters. The importance of discarding the initial filtrate or preflushing such filters before use is emphasized.
package_name Springer
publikationsjahr_anzeige 1990
publikationsjahr_facette 1990
publikationsjahr_intervall 8009:1990-1994
publikationsjahr_sort 1990
publisher Springer
reference 7 (1990), S. 347-350
schlagwort filter sterilization
culture media
embryotoxicity
search_space articles
shingle_author_1 Harrison, K. L.
Sherrin, D. A.
Hawthorne, T. A.
Breen, T. M.
West, G. A.
Wilson, L. M.
shingle_author_2 Harrison, K. L.
Sherrin, D. A.
Hawthorne, T. A.
Breen, T. M.
West, G. A.
Wilson, L. M.
shingle_author_3 Harrison, K. L.
Sherrin, D. A.
Hawthorne, T. A.
Breen, T. M.
West, G. A.
Wilson, L. M.
shingle_author_4 Harrison, K. L.
Sherrin, D. A.
Hawthorne, T. A.
Breen, T. M.
West, G. A.
Wilson, L. M.
shingle_catch_all_1 Harrison, K. L.
Sherrin, D. A.
Hawthorne, T. A.
Breen, T. M.
West, G. A.
Wilson, L. M.
Embryotoxicity of micropore filters used in liquid sterilization
filter sterilization
culture media
embryotoxicity
filter sterilization
culture media
embryotoxicity
Abstract Microfiltration is the usual method of sterilization of tissue culture media. The filtrates of eight commercial filters were tested for embryotoxicity using mouse embryo growth. Four of five disk filters tested showed embryotoxicity in the first 5 ml of filtrate and the three largercapacity filters showed embryotoxicity for up to the first 10 ml of filtrate. The ethylene oxide-sterilized filters appeared to give poorer results than the gamma-irradiated filters. The importance of discarding the initial filtrate or preflushing such filters before use is emphasized.
1573-7330
15737330
Springer
shingle_catch_all_2 Harrison, K. L.
Sherrin, D. A.
Hawthorne, T. A.
Breen, T. M.
West, G. A.
Wilson, L. M.
Embryotoxicity of micropore filters used in liquid sterilization
filter sterilization
culture media
embryotoxicity
filter sterilization
culture media
embryotoxicity
Abstract Microfiltration is the usual method of sterilization of tissue culture media. The filtrates of eight commercial filters were tested for embryotoxicity using mouse embryo growth. Four of five disk filters tested showed embryotoxicity in the first 5 ml of filtrate and the three largercapacity filters showed embryotoxicity for up to the first 10 ml of filtrate. The ethylene oxide-sterilized filters appeared to give poorer results than the gamma-irradiated filters. The importance of discarding the initial filtrate or preflushing such filters before use is emphasized.
1573-7330
15737330
Springer
shingle_catch_all_3 Harrison, K. L.
Sherrin, D. A.
Hawthorne, T. A.
Breen, T. M.
West, G. A.
Wilson, L. M.
Embryotoxicity of micropore filters used in liquid sterilization
filter sterilization
culture media
embryotoxicity
filter sterilization
culture media
embryotoxicity
Abstract Microfiltration is the usual method of sterilization of tissue culture media. The filtrates of eight commercial filters were tested for embryotoxicity using mouse embryo growth. Four of five disk filters tested showed embryotoxicity in the first 5 ml of filtrate and the three largercapacity filters showed embryotoxicity for up to the first 10 ml of filtrate. The ethylene oxide-sterilized filters appeared to give poorer results than the gamma-irradiated filters. The importance of discarding the initial filtrate or preflushing such filters before use is emphasized.
1573-7330
15737330
Springer
shingle_catch_all_4 Harrison, K. L.
Sherrin, D. A.
Hawthorne, T. A.
Breen, T. M.
West, G. A.
Wilson, L. M.
Embryotoxicity of micropore filters used in liquid sterilization
filter sterilization
culture media
embryotoxicity
filter sterilization
culture media
embryotoxicity
Abstract Microfiltration is the usual method of sterilization of tissue culture media. The filtrates of eight commercial filters were tested for embryotoxicity using mouse embryo growth. Four of five disk filters tested showed embryotoxicity in the first 5 ml of filtrate and the three largercapacity filters showed embryotoxicity for up to the first 10 ml of filtrate. The ethylene oxide-sterilized filters appeared to give poorer results than the gamma-irradiated filters. The importance of discarding the initial filtrate or preflushing such filters before use is emphasized.
1573-7330
15737330
Springer
shingle_title_1 Embryotoxicity of micropore filters used in liquid sterilization
shingle_title_2 Embryotoxicity of micropore filters used in liquid sterilization
shingle_title_3 Embryotoxicity of micropore filters used in liquid sterilization
shingle_title_4 Embryotoxicity of micropore filters used in liquid sterilization
sigel_instance_filter dkfz
geomar
wilbert
ipn
albert
fhp
source_archive Springer Online Journal Archives 1860-2000
timestamp 2024-05-06T09:59:30.665Z
titel Embryotoxicity of micropore filters used in liquid sterilization
titel_suche Embryotoxicity of micropore filters used in liquid sterilization
topic WW-YZ
uid nat_lic_papers_NLM197497195