Embryotoxicity of micropore filters used in liquid sterilization
Harrison, K. L. ; Sherrin, D. A. ; Hawthorne, T. A. ; Breen, T. M. ; West, G. A. ; Wilson, L. M.
Springer
Published 1990
Springer
Published 1990
ISSN: |
1573-7330
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Keywords: |
filter sterilization ; culture media ; embryotoxicity
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Source: |
Springer Online Journal Archives 1860-2000
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Topics: |
Medicine
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Notes: |
Abstract Microfiltration is the usual method of sterilization of tissue culture media. The filtrates of eight commercial filters were tested for embryotoxicity using mouse embryo growth. Four of five disk filters tested showed embryotoxicity in the first 5 ml of filtrate and the three largercapacity filters showed embryotoxicity for up to the first 10 ml of filtrate. The ethylene oxide-sterilized filters appeared to give poorer results than the gamma-irradiated filters. The importance of discarding the initial filtrate or preflushing such filters before use is emphasized.
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Type of Medium: |
Electronic Resource
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URL: |
_version_ | 1798296904510996481 |
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autor | Harrison, K. L. Sherrin, D. A. Hawthorne, T. A. Breen, T. M. West, G. A. Wilson, L. M. |
autorsonst | Harrison, K. L. Sherrin, D. A. Hawthorne, T. A. Breen, T. M. West, G. A. Wilson, L. M. |
book_url | http://dx.doi.org/10.1007/BF01130588 |
datenlieferant | nat_lic_papers |
hauptsatz | hsatz_simple |
identnr | NLM197497195 |
issn | 1573-7330 |
journal_name | Journal of assisted reproduction and genetics |
materialart | 1 |
notes | Abstract Microfiltration is the usual method of sterilization of tissue culture media. The filtrates of eight commercial filters were tested for embryotoxicity using mouse embryo growth. Four of five disk filters tested showed embryotoxicity in the first 5 ml of filtrate and the three largercapacity filters showed embryotoxicity for up to the first 10 ml of filtrate. The ethylene oxide-sterilized filters appeared to give poorer results than the gamma-irradiated filters. The importance of discarding the initial filtrate or preflushing such filters before use is emphasized. |
package_name | Springer |
publikationsjahr_anzeige | 1990 |
publikationsjahr_facette | 1990 |
publikationsjahr_intervall | 8009:1990-1994 |
publikationsjahr_sort | 1990 |
publisher | Springer |
reference | 7 (1990), S. 347-350 |
schlagwort | filter sterilization culture media embryotoxicity |
search_space | articles |
shingle_author_1 | Harrison, K. L. Sherrin, D. A. Hawthorne, T. A. Breen, T. M. West, G. A. Wilson, L. M. |
shingle_author_2 | Harrison, K. L. Sherrin, D. A. Hawthorne, T. A. Breen, T. M. West, G. A. Wilson, L. M. |
shingle_author_3 | Harrison, K. L. Sherrin, D. A. Hawthorne, T. A. Breen, T. M. West, G. A. Wilson, L. M. |
shingle_author_4 | Harrison, K. L. Sherrin, D. A. Hawthorne, T. A. Breen, T. M. West, G. A. Wilson, L. M. |
shingle_catch_all_1 | Harrison, K. L. Sherrin, D. A. Hawthorne, T. A. Breen, T. M. West, G. A. Wilson, L. M. Embryotoxicity of micropore filters used in liquid sterilization filter sterilization culture media embryotoxicity filter sterilization culture media embryotoxicity Abstract Microfiltration is the usual method of sterilization of tissue culture media. The filtrates of eight commercial filters were tested for embryotoxicity using mouse embryo growth. Four of five disk filters tested showed embryotoxicity in the first 5 ml of filtrate and the three largercapacity filters showed embryotoxicity for up to the first 10 ml of filtrate. The ethylene oxide-sterilized filters appeared to give poorer results than the gamma-irradiated filters. The importance of discarding the initial filtrate or preflushing such filters before use is emphasized. 1573-7330 15737330 Springer |
shingle_catch_all_2 | Harrison, K. L. Sherrin, D. A. Hawthorne, T. A. Breen, T. M. West, G. A. Wilson, L. M. Embryotoxicity of micropore filters used in liquid sterilization filter sterilization culture media embryotoxicity filter sterilization culture media embryotoxicity Abstract Microfiltration is the usual method of sterilization of tissue culture media. The filtrates of eight commercial filters were tested for embryotoxicity using mouse embryo growth. Four of five disk filters tested showed embryotoxicity in the first 5 ml of filtrate and the three largercapacity filters showed embryotoxicity for up to the first 10 ml of filtrate. The ethylene oxide-sterilized filters appeared to give poorer results than the gamma-irradiated filters. The importance of discarding the initial filtrate or preflushing such filters before use is emphasized. 1573-7330 15737330 Springer |
shingle_catch_all_3 | Harrison, K. L. Sherrin, D. A. Hawthorne, T. A. Breen, T. M. West, G. A. Wilson, L. M. Embryotoxicity of micropore filters used in liquid sterilization filter sterilization culture media embryotoxicity filter sterilization culture media embryotoxicity Abstract Microfiltration is the usual method of sterilization of tissue culture media. The filtrates of eight commercial filters were tested for embryotoxicity using mouse embryo growth. Four of five disk filters tested showed embryotoxicity in the first 5 ml of filtrate and the three largercapacity filters showed embryotoxicity for up to the first 10 ml of filtrate. The ethylene oxide-sterilized filters appeared to give poorer results than the gamma-irradiated filters. The importance of discarding the initial filtrate or preflushing such filters before use is emphasized. 1573-7330 15737330 Springer |
shingle_catch_all_4 | Harrison, K. L. Sherrin, D. A. Hawthorne, T. A. Breen, T. M. West, G. A. Wilson, L. M. Embryotoxicity of micropore filters used in liquid sterilization filter sterilization culture media embryotoxicity filter sterilization culture media embryotoxicity Abstract Microfiltration is the usual method of sterilization of tissue culture media. The filtrates of eight commercial filters were tested for embryotoxicity using mouse embryo growth. Four of five disk filters tested showed embryotoxicity in the first 5 ml of filtrate and the three largercapacity filters showed embryotoxicity for up to the first 10 ml of filtrate. The ethylene oxide-sterilized filters appeared to give poorer results than the gamma-irradiated filters. The importance of discarding the initial filtrate or preflushing such filters before use is emphasized. 1573-7330 15737330 Springer |
shingle_title_1 | Embryotoxicity of micropore filters used in liquid sterilization |
shingle_title_2 | Embryotoxicity of micropore filters used in liquid sterilization |
shingle_title_3 | Embryotoxicity of micropore filters used in liquid sterilization |
shingle_title_4 | Embryotoxicity of micropore filters used in liquid sterilization |
sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
source_archive | Springer Online Journal Archives 1860-2000 |
timestamp | 2024-05-06T09:59:30.665Z |
titel | Embryotoxicity of micropore filters used in liquid sterilization |
titel_suche | Embryotoxicity of micropore filters used in liquid sterilization |
topic | WW-YZ |
uid | nat_lic_papers_NLM197497195 |