urf13-T of T cytoplasm maize mitochondria encodes a 13 kD polypeptide

Wise, R. P. ; Fliss, A. E. ; Pring, D. R. ; Gengenbach, B. G.
Springer
Published 1987
ISSN:
1573-5028
Keywords:
cytoplasmic male sterility ; disease susceptibility ; Cochliobolus heterostrophus ; Phyllosticta maydis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Polyspecific antibody to a 17 amino acid synthetic peptide from the maize T-cytoplasm urf13-T mitochondrial open reading frame immunoprecipitated a 13 kD polypeptide from 35S-methionine incorporations of T cytoplasm maize. Male-fertile, toxin-insensitive mutants in which the urf13-T sequence is deleted do not synthesize the 13 kD polypeptide. A mutant designated T-4, which carries a 5 bp insertion and a premature stop codon, synthesizes a truncated polypeptide, corresponding to an open reading frame of 8.3 kD. Thus the 13 kD polypeptide is trunctated or absent in mutants expressing male fertility and toxin insensitivity in T-cytoplasm maize.
Type of Medium:
Electronic Resource
URL: