Import and processing of the precursor form of the gamma subunit of the chloroplast ATP synthase from tobacco
| ISSN: |
1573-5028
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| Keywords: |
ATP synthase ; presequence ; chloroplast import ; stromal processing peptidase
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| Source: |
Springer Online Journal Archives 1860-2000
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| Topics: |
Biology
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| Notes: |
Abstract A cDNA clone encoding the complete precursor of the γ subunit of chloroplast ATP synthase has been isolated from a tobacco (Nicotiana tabacum) leaf cDNA library in λgt11. The 1.4 kb insert encodes a polypeptide of 377 amino acid residues, of which 55 residues constitute an N-terminal presequence and 322 residues make up the mature γ subunit. Hybridisation of the cDNA to Southern blots of tobacco genomic DNA indicates the presence of two genes in the haploid genome. Transcription and translation of the cDNA in vitro produced a protein of 41 kDa which was imported by isolated pea chloroplasts and processed to the mature 36 kDa subunit. The γ subunit precursor was processed to the mature size by a processing peptidase of 180 kDa present in pea stromal extracts.
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| Type of Medium: |
Electronic Resource
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| URL: |