Primary culture of mucin-producing cells from chicken trachea
| ISSN: |
1573-0603
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| Keywords: |
trachea ; mucin-producing cells ; primary culture ; differentiated epithelial cells
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| Source: |
Springer Online Journal Archives 1860-2000
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| Topics: |
Biology
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| Notes: |
Summary Tracheae obtained from the domestic fowl were incubated with a pronase/EDTA mixture to dissociate the epithelial cells. The freshly isolated cells were placed on collagen-coated coverglasses in RPMI-1640 medium supplemented with 10% fetal bovine serum and incubated in a humidified atmosphere of 5% CO2 in air at 37°C. Mucin-producing cells as well as basal cells were present in the cultures which reached confluency within 3 d. This primary cell culture system may prove useful for thein vitro study of mucin synthesis and secretion.
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| Type of Medium: |
Electronic Resource
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| URL: |