Alterations in rabbit retina lipid metabolism induced by detachment
Santos, Fernando F. ; Turco, Elena B. Rodriguez ; Gordon, William C. ; Peyman, Gholam A. ; Bazan, Nicolas G.
Springer
Published 1995
Springer
Published 1995
| ISSN: |
1573-2630
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|---|---|
| Keywords: |
docosahexaenoic acid ; lipid metabolism ; phospholipids ; rabbit retina ; retinal detachment ; triacylglycerol
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| Source: |
Springer Online Journal Archives 1860-2000
|
| Topics: |
Medicine
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| Notes: |
Abstract Background Docosahexaenoic acid (22 ∶ 6n-3, DHA) is found in high concentration in phospholipids from retinal membranes, and is essential for their function. This study investigated the effect of in vivo retinal detachment on in vitro lipid metabolism using [3H]DHA. Methods Rabbit retina was detached from the retinal pigment epithelium by injecting physiological saline into the subretinal space of the eye. Retinal samples from control (non-operated) and sham (operated, no detachment) animals, and from attached and detached retinal areas from the same eye, were incubated in vitro with [3H]DHA for 4 hours, and then prepared for biochemical and autoradiographic analysis. Results In control and sham retinas, [3H]DHA was preferentially esterified into phospholipids (82%) with low labeling of free fatty acids (FFA) (5%). In samples from detached areas of the retina, a higher proportion of [3H]DHA was recovered in the FFA pool (up to 30%) and its esterification was shunted into triacylglycerol, thereby reducing the formation of [3H]DHA-phospholipids. Changes were sustained through 48 hours of postdetachment. High labeling of inner segments and synaptic terminals was observed autoradiographically in control retinas, while in detached retinas, clusters of labeling were detected in the neural retina, and eventually within the photoreceptor layer. Conclusion Retinal detachment induces longlasting changes in lipid metabolism which are reflected in lower labeling of [3H]DHA-phospholipids. Metabolic changes, sustained through 48 hours, may lead to inadequate synthesis/turnover of phospholipids, among them, those containing DHA, possibly resulting in defective disc membrane assembly with subsequent deterioration of visual cells.
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| Type of Medium: |
Electronic Resource
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| URL: |
| _version_ | 1798296654005141504 |
|---|---|
| autor | Santos, Fernando F. Turco, Elena B. Rodriguez Gordon, William C. Peyman, Gholam A. Bazan, Nicolas G. |
| autorsonst | Santos, Fernando F. Turco, Elena B. Rodriguez Gordon, William C. Peyman, Gholam A. Bazan, Nicolas G. |
| book_url | http://dx.doi.org/10.1007/BF00133731 |
| datenlieferant | nat_lic_papers |
| hauptsatz | hsatz_simple |
| identnr | NLM194446433 |
| issn | 1573-2630 |
| journal_name | International ophthalmology |
| materialart | 1 |
| notes | Abstract Background Docosahexaenoic acid (22 ∶ 6n-3, DHA) is found in high concentration in phospholipids from retinal membranes, and is essential for their function. This study investigated the effect of in vivo retinal detachment on in vitro lipid metabolism using [3H]DHA. Methods Rabbit retina was detached from the retinal pigment epithelium by injecting physiological saline into the subretinal space of the eye. Retinal samples from control (non-operated) and sham (operated, no detachment) animals, and from attached and detached retinal areas from the same eye, were incubated in vitro with [3H]DHA for 4 hours, and then prepared for biochemical and autoradiographic analysis. Results In control and sham retinas, [3H]DHA was preferentially esterified into phospholipids (82%) with low labeling of free fatty acids (FFA) (5%). In samples from detached areas of the retina, a higher proportion of [3H]DHA was recovered in the FFA pool (up to 30%) and its esterification was shunted into triacylglycerol, thereby reducing the formation of [3H]DHA-phospholipids. Changes were sustained through 48 hours of postdetachment. High labeling of inner segments and synaptic terminals was observed autoradiographically in control retinas, while in detached retinas, clusters of labeling were detected in the neural retina, and eventually within the photoreceptor layer. Conclusion Retinal detachment induces longlasting changes in lipid metabolism which are reflected in lower labeling of [3H]DHA-phospholipids. Metabolic changes, sustained through 48 hours, may lead to inadequate synthesis/turnover of phospholipids, among them, those containing DHA, possibly resulting in defective disc membrane assembly with subsequent deterioration of visual cells. |
| package_name | Springer |
| publikationsjahr_anzeige | 1995 |
| publikationsjahr_facette | 1995 |
| publikationsjahr_intervall | 8004:1995-1999 |
| publikationsjahr_sort | 1995 |
| publisher | Springer |
| reference | 19 (1995), S. 149-159 |
| schlagwort | docosahexaenoic acid lipid metabolism phospholipids rabbit retina retinal detachment triacylglycerol |
| search_space | articles |
| shingle_author_1 | Santos, Fernando F. Turco, Elena B. Rodriguez Gordon, William C. Peyman, Gholam A. Bazan, Nicolas G. |
| shingle_author_2 | Santos, Fernando F. Turco, Elena B. Rodriguez Gordon, William C. Peyman, Gholam A. Bazan, Nicolas G. |
| shingle_author_3 | Santos, Fernando F. Turco, Elena B. Rodriguez Gordon, William C. Peyman, Gholam A. Bazan, Nicolas G. |
| shingle_author_4 | Santos, Fernando F. Turco, Elena B. Rodriguez Gordon, William C. Peyman, Gholam A. Bazan, Nicolas G. |
| shingle_catch_all_1 | Santos, Fernando F. Turco, Elena B. Rodriguez Gordon, William C. Peyman, Gholam A. Bazan, Nicolas G. Alterations in rabbit retina lipid metabolism induced by detachment docosahexaenoic acid lipid metabolism phospholipids rabbit retina retinal detachment triacylglycerol docosahexaenoic acid lipid metabolism phospholipids rabbit retina retinal detachment triacylglycerol Abstract Background Docosahexaenoic acid (22 ∶ 6n-3, DHA) is found in high concentration in phospholipids from retinal membranes, and is essential for their function. This study investigated the effect of in vivo retinal detachment on in vitro lipid metabolism using [3H]DHA. Methods Rabbit retina was detached from the retinal pigment epithelium by injecting physiological saline into the subretinal space of the eye. Retinal samples from control (non-operated) and sham (operated, no detachment) animals, and from attached and detached retinal areas from the same eye, were incubated in vitro with [3H]DHA for 4 hours, and then prepared for biochemical and autoradiographic analysis. Results In control and sham retinas, [3H]DHA was preferentially esterified into phospholipids (82%) with low labeling of free fatty acids (FFA) (5%). In samples from detached areas of the retina, a higher proportion of [3H]DHA was recovered in the FFA pool (up to 30%) and its esterification was shunted into triacylglycerol, thereby reducing the formation of [3H]DHA-phospholipids. Changes were sustained through 48 hours of postdetachment. High labeling of inner segments and synaptic terminals was observed autoradiographically in control retinas, while in detached retinas, clusters of labeling were detected in the neural retina, and eventually within the photoreceptor layer. Conclusion Retinal detachment induces longlasting changes in lipid metabolism which are reflected in lower labeling of [3H]DHA-phospholipids. Metabolic changes, sustained through 48 hours, may lead to inadequate synthesis/turnover of phospholipids, among them, those containing DHA, possibly resulting in defective disc membrane assembly with subsequent deterioration of visual cells. 1573-2630 15732630 Springer |
| shingle_catch_all_2 | Santos, Fernando F. Turco, Elena B. Rodriguez Gordon, William C. Peyman, Gholam A. Bazan, Nicolas G. Alterations in rabbit retina lipid metabolism induced by detachment docosahexaenoic acid lipid metabolism phospholipids rabbit retina retinal detachment triacylglycerol docosahexaenoic acid lipid metabolism phospholipids rabbit retina retinal detachment triacylglycerol Abstract Background Docosahexaenoic acid (22 ∶ 6n-3, DHA) is found in high concentration in phospholipids from retinal membranes, and is essential for their function. This study investigated the effect of in vivo retinal detachment on in vitro lipid metabolism using [3H]DHA. Methods Rabbit retina was detached from the retinal pigment epithelium by injecting physiological saline into the subretinal space of the eye. Retinal samples from control (non-operated) and sham (operated, no detachment) animals, and from attached and detached retinal areas from the same eye, were incubated in vitro with [3H]DHA for 4 hours, and then prepared for biochemical and autoradiographic analysis. Results In control and sham retinas, [3H]DHA was preferentially esterified into phospholipids (82%) with low labeling of free fatty acids (FFA) (5%). In samples from detached areas of the retina, a higher proportion of [3H]DHA was recovered in the FFA pool (up to 30%) and its esterification was shunted into triacylglycerol, thereby reducing the formation of [3H]DHA-phospholipids. Changes were sustained through 48 hours of postdetachment. High labeling of inner segments and synaptic terminals was observed autoradiographically in control retinas, while in detached retinas, clusters of labeling were detected in the neural retina, and eventually within the photoreceptor layer. Conclusion Retinal detachment induces longlasting changes in lipid metabolism which are reflected in lower labeling of [3H]DHA-phospholipids. Metabolic changes, sustained through 48 hours, may lead to inadequate synthesis/turnover of phospholipids, among them, those containing DHA, possibly resulting in defective disc membrane assembly with subsequent deterioration of visual cells. 1573-2630 15732630 Springer |
| shingle_catch_all_3 | Santos, Fernando F. Turco, Elena B. Rodriguez Gordon, William C. Peyman, Gholam A. Bazan, Nicolas G. Alterations in rabbit retina lipid metabolism induced by detachment docosahexaenoic acid lipid metabolism phospholipids rabbit retina retinal detachment triacylglycerol docosahexaenoic acid lipid metabolism phospholipids rabbit retina retinal detachment triacylglycerol Abstract Background Docosahexaenoic acid (22 ∶ 6n-3, DHA) is found in high concentration in phospholipids from retinal membranes, and is essential for their function. This study investigated the effect of in vivo retinal detachment on in vitro lipid metabolism using [3H]DHA. Methods Rabbit retina was detached from the retinal pigment epithelium by injecting physiological saline into the subretinal space of the eye. Retinal samples from control (non-operated) and sham (operated, no detachment) animals, and from attached and detached retinal areas from the same eye, were incubated in vitro with [3H]DHA for 4 hours, and then prepared for biochemical and autoradiographic analysis. Results In control and sham retinas, [3H]DHA was preferentially esterified into phospholipids (82%) with low labeling of free fatty acids (FFA) (5%). In samples from detached areas of the retina, a higher proportion of [3H]DHA was recovered in the FFA pool (up to 30%) and its esterification was shunted into triacylglycerol, thereby reducing the formation of [3H]DHA-phospholipids. Changes were sustained through 48 hours of postdetachment. High labeling of inner segments and synaptic terminals was observed autoradiographically in control retinas, while in detached retinas, clusters of labeling were detected in the neural retina, and eventually within the photoreceptor layer. Conclusion Retinal detachment induces longlasting changes in lipid metabolism which are reflected in lower labeling of [3H]DHA-phospholipids. Metabolic changes, sustained through 48 hours, may lead to inadequate synthesis/turnover of phospholipids, among them, those containing DHA, possibly resulting in defective disc membrane assembly with subsequent deterioration of visual cells. 1573-2630 15732630 Springer |
| shingle_catch_all_4 | Santos, Fernando F. Turco, Elena B. Rodriguez Gordon, William C. Peyman, Gholam A. Bazan, Nicolas G. Alterations in rabbit retina lipid metabolism induced by detachment docosahexaenoic acid lipid metabolism phospholipids rabbit retina retinal detachment triacylglycerol docosahexaenoic acid lipid metabolism phospholipids rabbit retina retinal detachment triacylglycerol Abstract Background Docosahexaenoic acid (22 ∶ 6n-3, DHA) is found in high concentration in phospholipids from retinal membranes, and is essential for their function. This study investigated the effect of in vivo retinal detachment on in vitro lipid metabolism using [3H]DHA. Methods Rabbit retina was detached from the retinal pigment epithelium by injecting physiological saline into the subretinal space of the eye. Retinal samples from control (non-operated) and sham (operated, no detachment) animals, and from attached and detached retinal areas from the same eye, were incubated in vitro with [3H]DHA for 4 hours, and then prepared for biochemical and autoradiographic analysis. Results In control and sham retinas, [3H]DHA was preferentially esterified into phospholipids (82%) with low labeling of free fatty acids (FFA) (5%). In samples from detached areas of the retina, a higher proportion of [3H]DHA was recovered in the FFA pool (up to 30%) and its esterification was shunted into triacylglycerol, thereby reducing the formation of [3H]DHA-phospholipids. Changes were sustained through 48 hours of postdetachment. High labeling of inner segments and synaptic terminals was observed autoradiographically in control retinas, while in detached retinas, clusters of labeling were detected in the neural retina, and eventually within the photoreceptor layer. Conclusion Retinal detachment induces longlasting changes in lipid metabolism which are reflected in lower labeling of [3H]DHA-phospholipids. Metabolic changes, sustained through 48 hours, may lead to inadequate synthesis/turnover of phospholipids, among them, those containing DHA, possibly resulting in defective disc membrane assembly with subsequent deterioration of visual cells. 1573-2630 15732630 Springer |
| shingle_title_1 | Alterations in rabbit retina lipid metabolism induced by detachment |
| shingle_title_2 | Alterations in rabbit retina lipid metabolism induced by detachment |
| shingle_title_3 | Alterations in rabbit retina lipid metabolism induced by detachment |
| shingle_title_4 | Alterations in rabbit retina lipid metabolism induced by detachment |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Springer Online Journal Archives 1860-2000 |
| timestamp | 2024-05-06T09:55:31.938Z |
| titel | Alterations in rabbit retina lipid metabolism induced by detachment |
| titel_suche | Alterations in rabbit retina lipid metabolism induced by detachment |
| topic | WW-YZ |
| uid | nat_lic_papers_NLM194446433 |