Determination of interleukin 2 activity by a new fluorometric method
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1573-6784
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| Source: |
Springer Online Journal Archives 1860-2000
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| Topics: |
Process Engineering, Biotechnology, Nutrition Technology
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| Notes: |
Summary Interleukin 2 activity is usually determined by a proliferation assay using an IL-2-dependent cell line. Tritiated thymidine incorporation during DNA synthesis is a suitable method for this purpose, but its main drawback is the use of radioactive isotopes. We describe the use of Alamar Blue, a new fluorogenic growth indicator, for the measurement of interleukin 2 activity in microtitration plates. This assay is sensitive and economical. The lower limit of detection is about 400 cells per well with an intra-assay coefficient of variation of about 5 percent.
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| Type of Medium: |
Electronic Resource
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| URL: |