The use of EDTA or polymyxin with lysozyme for the recovery of intracellular products fromEscherichia. coli
| ISSN: |
1573-6784
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| Source: |
Springer Online Journal Archives 1860-2000
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| Topics: |
Process Engineering, Biotechnology, Nutrition Technology
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| Notes: |
Summary Escherichia coli cells taken from exponential and late stationary (or decline) phases of culture were very susceptible to lysis by EDTA/lysozyme. Log phase cells were most susceptible to lysis by polymyxin/lysozyme. Treatment ofE. coli with EDTA and lysozyme compared favourably with sonication as a method for release of intracellular protein. Concentration ranges for optimal lysis were 100–800 μg/ml for EDTA and 25–50 μg/ml for lysozyme.
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| Type of Medium: |
Electronic Resource
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| URL: |