Culture of PNKT-4B cells at invasion permissive and restrictive temperatures. I. Chromosomal analysis
Williams, J. W. ; Smith, M. D. ; Carlson, D. L. ; Williams, C. S. ; Tweedell, K. S. ; McKinnell, R. G.
Springer
Published 1990
Springer
Published 1990
| ISSN: |
1573-7276
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|---|---|
| Source: |
Springer Online Journal Archives 1860-2000
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| Topics: |
Medicine
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| Notes: |
Abstract PNKT-4B is an aneuploid cell line derived from a herpesvirus-induced renal adenocarcinoma of Rana pipiens that displays restricted invasion at 21°C or cooler and invasion at 23°C through 28°C. Metaphase chromosomes obtained from subcultures (passages 297; 345–347) grown at 18°C or 28°C were Giemsa stained or N-banded with acidic silver nitrate. Cells grown at 18°C displayed a modal chromosome number of 41, while 28°C cultures displayed a modal number of 40. The distribution of the chromosomes suggests that the two temperatures may be allowing growth of different subclonal populations. N-banding of chromosomes at both temperatures revealed an increase of active nucleolar organizer regions (NORs) over normal frog tissues, 2/2N. Analysis of 200 N-banded spreads from cells grown at each temperature revealed modal numbers of 9 NORs/cell and modal numbers of 6 NOR-containing chromosomes/cell. Nine specific NOR-containing chromosomes were identified and scored. Similar distributions were observed at 18°C and 28°C. The data imply that the modal number of PNKT-4B has shifted since it was first described, 39, and differs at invasion-permissive and — restrictive temperatures. Increased numbers of active NORs and alterations of NOR-containing chromosomes imply an amplification of rDNA over the amount in normal frog.
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| Type of Medium: |
Electronic Resource
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| URL: |
| _version_ | 1798296899820716033 |
|---|---|
| autor | Williams, J. W. Smith, M. D. Carlson, D. L. Williams, C. S. Tweedell, K. S. McKinnell, R. G. |
| autorsonst | Williams, J. W. Smith, M. D. Carlson, D. L. Williams, C. S. Tweedell, K. S. McKinnell, R. G. |
| book_url | http://dx.doi.org/10.1007/BF00141257 |
| datenlieferant | nat_lic_papers |
| hauptsatz | hsatz_simple |
| identnr | NLM193234416 |
| iqvoc_descriptor_title | iqvoc_00000708:analysis |
| issn | 1573-7276 |
| journal_name | Clinical & experimental metastasis |
| materialart | 1 |
| notes | Abstract PNKT-4B is an aneuploid cell line derived from a herpesvirus-induced renal adenocarcinoma of Rana pipiens that displays restricted invasion at 21°C or cooler and invasion at 23°C through 28°C. Metaphase chromosomes obtained from subcultures (passages 297; 345–347) grown at 18°C or 28°C were Giemsa stained or N-banded with acidic silver nitrate. Cells grown at 18°C displayed a modal chromosome number of 41, while 28°C cultures displayed a modal number of 40. The distribution of the chromosomes suggests that the two temperatures may be allowing growth of different subclonal populations. N-banding of chromosomes at both temperatures revealed an increase of active nucleolar organizer regions (NORs) over normal frog tissues, 2/2N. Analysis of 200 N-banded spreads from cells grown at each temperature revealed modal numbers of 9 NORs/cell and modal numbers of 6 NOR-containing chromosomes/cell. Nine specific NOR-containing chromosomes were identified and scored. Similar distributions were observed at 18°C and 28°C. The data imply that the modal number of PNKT-4B has shifted since it was first described, 39, and differs at invasion-permissive and — restrictive temperatures. Increased numbers of active NORs and alterations of NOR-containing chromosomes imply an amplification of rDNA over the amount in normal frog. |
| package_name | Springer |
| publikationsjahr_anzeige | 1990 |
| publikationsjahr_facette | 1990 |
| publikationsjahr_intervall | 8009:1990-1994 |
| publikationsjahr_sort | 1990 |
| publisher | Springer |
| reference | 8 (1990), S. 267-276 |
| search_space | articles |
| shingle_author_1 | Williams, J. W. Smith, M. D. Carlson, D. L. Williams, C. S. Tweedell, K. S. McKinnell, R. G. |
| shingle_author_2 | Williams, J. W. Smith, M. D. Carlson, D. L. Williams, C. S. Tweedell, K. S. McKinnell, R. G. |
| shingle_author_3 | Williams, J. W. Smith, M. D. Carlson, D. L. Williams, C. S. Tweedell, K. S. McKinnell, R. G. |
| shingle_author_4 | Williams, J. W. Smith, M. D. Carlson, D. L. Williams, C. S. Tweedell, K. S. McKinnell, R. G. |
| shingle_catch_all_1 | Williams, J. W. Smith, M. D. Carlson, D. L. Williams, C. S. Tweedell, K. S. McKinnell, R. G. Culture of PNKT-4B cells at invasion permissive and restrictive temperatures. I. Chromosomal analysis Abstract PNKT-4B is an aneuploid cell line derived from a herpesvirus-induced renal adenocarcinoma of Rana pipiens that displays restricted invasion at 21°C or cooler and invasion at 23°C through 28°C. Metaphase chromosomes obtained from subcultures (passages 297; 345–347) grown at 18°C or 28°C were Giemsa stained or N-banded with acidic silver nitrate. Cells grown at 18°C displayed a modal chromosome number of 41, while 28°C cultures displayed a modal number of 40. The distribution of the chromosomes suggests that the two temperatures may be allowing growth of different subclonal populations. N-banding of chromosomes at both temperatures revealed an increase of active nucleolar organizer regions (NORs) over normal frog tissues, 2/2N. Analysis of 200 N-banded spreads from cells grown at each temperature revealed modal numbers of 9 NORs/cell and modal numbers of 6 NOR-containing chromosomes/cell. Nine specific NOR-containing chromosomes were identified and scored. Similar distributions were observed at 18°C and 28°C. The data imply that the modal number of PNKT-4B has shifted since it was first described, 39, and differs at invasion-permissive and — restrictive temperatures. Increased numbers of active NORs and alterations of NOR-containing chromosomes imply an amplification of rDNA over the amount in normal frog. 1573-7276 15737276 Springer |
| shingle_catch_all_2 | Williams, J. W. Smith, M. D. Carlson, D. L. Williams, C. S. Tweedell, K. S. McKinnell, R. G. Culture of PNKT-4B cells at invasion permissive and restrictive temperatures. I. Chromosomal analysis Abstract PNKT-4B is an aneuploid cell line derived from a herpesvirus-induced renal adenocarcinoma of Rana pipiens that displays restricted invasion at 21°C or cooler and invasion at 23°C through 28°C. Metaphase chromosomes obtained from subcultures (passages 297; 345–347) grown at 18°C or 28°C were Giemsa stained or N-banded with acidic silver nitrate. Cells grown at 18°C displayed a modal chromosome number of 41, while 28°C cultures displayed a modal number of 40. The distribution of the chromosomes suggests that the two temperatures may be allowing growth of different subclonal populations. N-banding of chromosomes at both temperatures revealed an increase of active nucleolar organizer regions (NORs) over normal frog tissues, 2/2N. Analysis of 200 N-banded spreads from cells grown at each temperature revealed modal numbers of 9 NORs/cell and modal numbers of 6 NOR-containing chromosomes/cell. Nine specific NOR-containing chromosomes were identified and scored. Similar distributions were observed at 18°C and 28°C. The data imply that the modal number of PNKT-4B has shifted since it was first described, 39, and differs at invasion-permissive and — restrictive temperatures. Increased numbers of active NORs and alterations of NOR-containing chromosomes imply an amplification of rDNA over the amount in normal frog. 1573-7276 15737276 Springer |
| shingle_catch_all_3 | Williams, J. W. Smith, M. D. Carlson, D. L. Williams, C. S. Tweedell, K. S. McKinnell, R. G. Culture of PNKT-4B cells at invasion permissive and restrictive temperatures. I. Chromosomal analysis Abstract PNKT-4B is an aneuploid cell line derived from a herpesvirus-induced renal adenocarcinoma of Rana pipiens that displays restricted invasion at 21°C or cooler and invasion at 23°C through 28°C. Metaphase chromosomes obtained from subcultures (passages 297; 345–347) grown at 18°C or 28°C were Giemsa stained or N-banded with acidic silver nitrate. Cells grown at 18°C displayed a modal chromosome number of 41, while 28°C cultures displayed a modal number of 40. The distribution of the chromosomes suggests that the two temperatures may be allowing growth of different subclonal populations. N-banding of chromosomes at both temperatures revealed an increase of active nucleolar organizer regions (NORs) over normal frog tissues, 2/2N. Analysis of 200 N-banded spreads from cells grown at each temperature revealed modal numbers of 9 NORs/cell and modal numbers of 6 NOR-containing chromosomes/cell. Nine specific NOR-containing chromosomes were identified and scored. Similar distributions were observed at 18°C and 28°C. The data imply that the modal number of PNKT-4B has shifted since it was first described, 39, and differs at invasion-permissive and — restrictive temperatures. Increased numbers of active NORs and alterations of NOR-containing chromosomes imply an amplification of rDNA over the amount in normal frog. 1573-7276 15737276 Springer |
| shingle_catch_all_4 | Williams, J. W. Smith, M. D. Carlson, D. L. Williams, C. S. Tweedell, K. S. McKinnell, R. G. Culture of PNKT-4B cells at invasion permissive and restrictive temperatures. I. Chromosomal analysis Abstract PNKT-4B is an aneuploid cell line derived from a herpesvirus-induced renal adenocarcinoma of Rana pipiens that displays restricted invasion at 21°C or cooler and invasion at 23°C through 28°C. Metaphase chromosomes obtained from subcultures (passages 297; 345–347) grown at 18°C or 28°C were Giemsa stained or N-banded with acidic silver nitrate. Cells grown at 18°C displayed a modal chromosome number of 41, while 28°C cultures displayed a modal number of 40. The distribution of the chromosomes suggests that the two temperatures may be allowing growth of different subclonal populations. N-banding of chromosomes at both temperatures revealed an increase of active nucleolar organizer regions (NORs) over normal frog tissues, 2/2N. Analysis of 200 N-banded spreads from cells grown at each temperature revealed modal numbers of 9 NORs/cell and modal numbers of 6 NOR-containing chromosomes/cell. Nine specific NOR-containing chromosomes were identified and scored. Similar distributions were observed at 18°C and 28°C. The data imply that the modal number of PNKT-4B has shifted since it was first described, 39, and differs at invasion-permissive and — restrictive temperatures. Increased numbers of active NORs and alterations of NOR-containing chromosomes imply an amplification of rDNA over the amount in normal frog. 1573-7276 15737276 Springer |
| shingle_title_1 | Culture of PNKT-4B cells at invasion permissive and restrictive temperatures. I. Chromosomal analysis |
| shingle_title_2 | Culture of PNKT-4B cells at invasion permissive and restrictive temperatures. I. Chromosomal analysis |
| shingle_title_3 | Culture of PNKT-4B cells at invasion permissive and restrictive temperatures. I. Chromosomal analysis |
| shingle_title_4 | Culture of PNKT-4B cells at invasion permissive and restrictive temperatures. I. Chromosomal analysis |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Springer Online Journal Archives 1860-2000 |
| timestamp | 2024-05-06T09:59:26.632Z |
| titel | Culture of PNKT-4B cells at invasion permissive and restrictive temperatures. I. Chromosomal analysis |
| titel_suche | Culture of PNKT-4B cells at invasion permissive and restrictive temperatures. I. Chromosomal analysis |
| topic | WW-YZ |
| uid | nat_lic_papers_NLM193234416 |