Effects of three inhibitors of nitric oxide synthase on host resistance to bacterial infection

1420-908X

NG-nitro-L-arginine-methyl-ester (L-NAME) ; NG-monomethyl-L-arginine (L-NMMA) ; N-iminoethyl-L-ornithine (L-NIO) ; Septic shock ; Survival

Springer Online Journal Archives 1860-2000

Medicine

Abstract Objective: To examine the effects of three nitric oxide synthase inhibitors on survival in a murine sepsis model.Design: Prospective randomized experimental trials.Setting: Laboratory.Subjects: Female Balb/c mice.Interventions:Escherichia coli (108 colony-formingunits/body) were injected into the peritoneal cavities of Balb/c mice. NG-nitro-L-arginine-methyl-ester, NG-monomethyl-L-arginine, or N-iminoethyl-L-ornithine was given at various concentrations, intraperitoneally, one hour before bacterial challenge.Measurements: One hundred and fifteen animals were observed for survival.Results: These inhibitors provided the mice no protection from the bacterial challenge. Notably, pretreatment with NG-nitro-L-arginine-methyl-ester (100 mg/kg i.p.) actually reduced survival time afterE. coli challenge.Conclusions: Inhibition of nitric oxide production improved neither the survival time nor rate in this murine sepsis model.

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