Studies of formation of bivalent metal ion complexes with poly-U
Sorokin, Victor A. ; Levchenko, Irina V. ; Valeev, Vladimir A. ; Gladchenko, Galina O. ; Blagoi, Yuri P. ; Li, Ren G.
New York : Wiley-Blackwell
Published 1989
New York : Wiley-Blackwell
Published 1989
| ISSN: |
0025-116X
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|---|---|
| Keywords: |
Chemistry ; Polymer and Materials Science
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| Source: |
Wiley InterScience Backfile Collection 1832-2000
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| Topics: |
Chemistry and Pharmacology
Physics
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| Notes: |
Differential UV spectroscopy is employed to investigate the interaction of poly-U with Mg2+, Ca2+, Mn2+ and Cu2+ ions in aqueous solution. Mg2+ and Mn2+ ions are observed to bind only to the polynucleotide phosphates, while Cu2+ and Ca2+ also interact with the poly-U bases. The most probable coordination sites are O(4) and also N(3) for Cu2+ ions. N(3) is a coordination site of Cu2+ ions in UMP and uridine. The rest of the ions do not interact with the bases of the free monomers. Unlike the non-cooperative Cu2+-UMP binding, the ion interaction (including Ca2+) with the poly-U bases is characterized by a positive cooperativity. The association constant of Ca2+ (45 1.mol-1) is 45 times lower than that of Cu2+.
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| Additional Material: |
4 Ill.
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| Type of Medium: |
Electronic Resource
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| URL: |