Differential effects of interferon gamma and alpha on in vitro model of angiogenesis
Maheshwari, R. K. ; Srikantan, V. ; Bhartiya, D. ; Kleinman, H. K. ; Grant, D. S.
New York, NY [u.a.] : Wiley-Blackwell
Published 1991
New York, NY [u.a.] : Wiley-Blackwell
Published 1991
| ISSN: |
0021-9541
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|---|---|
| Keywords: |
Life and Medical Sciences ; Cell & Developmental Biology
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| Source: |
Wiley InterScience Backfile Collection 1832-2000
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| Topics: |
Biology
Medicine
|
| Notes: |
The formation of blood vessels in vivo (angiogenesis) is an important process and is usually initiated in response to injury, tumor growth, or normal tissue development. We have studied the effect of human interferon (IFN) alpha (α) and gamma (γ) on the capillary-like network formation in an in vitro model of angiogenesis using human umbilical vein endothelial cells (HUVEC). When HUVEC cells are plated on Matrigel (reconstituted basement membrane matrix enriched in laminin), a network of capillary like structures (endotubes) rapidly forms. IFN-α enhanced the tube formation in a dose-dependent manner, whereas IFN-γ significantly inhibited the tube formation. In addition, both the enhancement and inhibition of angiogenesis by IFN-α and γ was found to be greater if the cells were pretreated with IFN for 12 hr before plating on the Matrigel. These results suggest that IFN may play an important role in several vascular processes including early stages of wound healing, recanalization of thrombi, tumor growth, metastasis, normal growth, and development.
|
| Additional Material: |
6 Ill.
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| Type of Medium: |
Electronic Resource
|
| URL: |
| _version_ | 1798297994638917633 |
|---|---|
| addmaterial | 6 Ill. |
| autor | Maheshwari, R. K. Srikantan, V. Bhartiya, D. Kleinman, H. K. Grant, D. S. |
| autorsonst | Maheshwari, R. K. Srikantan, V. Bhartiya, D. Kleinman, H. K. Grant, D. S. |
| book_url | http://dx.doi.org/10.1002/jcp.1041460121 |
| datenlieferant | nat_lic_papers |
| hauptsatz | hsatz_simple |
| identnr | NLM160803977 |
| issn | 0021-9541 |
| journal_name | Journal of Cellular Physiology |
| materialart | 1 |
| notes | The formation of blood vessels in vivo (angiogenesis) is an important process and is usually initiated in response to injury, tumor growth, or normal tissue development. We have studied the effect of human interferon (IFN) alpha (α) and gamma (γ) on the capillary-like network formation in an in vitro model of angiogenesis using human umbilical vein endothelial cells (HUVEC). When HUVEC cells are plated on Matrigel (reconstituted basement membrane matrix enriched in laminin), a network of capillary like structures (endotubes) rapidly forms. IFN-α enhanced the tube formation in a dose-dependent manner, whereas IFN-γ significantly inhibited the tube formation. In addition, both the enhancement and inhibition of angiogenesis by IFN-α and γ was found to be greater if the cells were pretreated with IFN for 12 hr before plating on the Matrigel. These results suggest that IFN may play an important role in several vascular processes including early stages of wound healing, recanalization of thrombi, tumor growth, metastasis, normal growth, and development. |
| package_name | Wiley-Blackwell |
| publikationsjahr_anzeige | 1991 |
| publikationsjahr_facette | 1991 |
| publikationsjahr_intervall | 8009:1990-1994 |
| publikationsjahr_sort | 1991 |
| publikationsort | New York, NY [u.a.] |
| publisher | Wiley-Blackwell |
| reference | 146 (1991), S. 164-169 |
| schlagwort | Life and Medical Sciences Cell & Developmental Biology |
| search_space | articles |
| shingle_author_1 | Maheshwari, R. K. Srikantan, V. Bhartiya, D. Kleinman, H. K. Grant, D. S. |
| shingle_author_2 | Maheshwari, R. K. Srikantan, V. Bhartiya, D. Kleinman, H. K. Grant, D. S. |
| shingle_author_3 | Maheshwari, R. K. Srikantan, V. Bhartiya, D. Kleinman, H. K. Grant, D. S. |
| shingle_author_4 | Maheshwari, R. K. Srikantan, V. Bhartiya, D. Kleinman, H. K. Grant, D. S. |
| shingle_catch_all_1 | Maheshwari, R. K. Srikantan, V. Bhartiya, D. Kleinman, H. K. Grant, D. S. Differential effects of interferon gamma and alpha on in vitro model of angiogenesis Life and Medical Sciences Cell & Developmental Biology Life and Medical Sciences Cell & Developmental Biology The formation of blood vessels in vivo (angiogenesis) is an important process and is usually initiated in response to injury, tumor growth, or normal tissue development. We have studied the effect of human interferon (IFN) alpha (α) and gamma (γ) on the capillary-like network formation in an in vitro model of angiogenesis using human umbilical vein endothelial cells (HUVEC). When HUVEC cells are plated on Matrigel (reconstituted basement membrane matrix enriched in laminin), a network of capillary like structures (endotubes) rapidly forms. IFN-α enhanced the tube formation in a dose-dependent manner, whereas IFN-γ significantly inhibited the tube formation. In addition, both the enhancement and inhibition of angiogenesis by IFN-α and γ was found to be greater if the cells were pretreated with IFN for 12 hr before plating on the Matrigel. These results suggest that IFN may play an important role in several vascular processes including early stages of wound healing, recanalization of thrombi, tumor growth, metastasis, normal growth, and development. 0021-9541 00219541 Wiley-Blackwell |
| shingle_catch_all_2 | Maheshwari, R. K. Srikantan, V. Bhartiya, D. Kleinman, H. K. Grant, D. S. Differential effects of interferon gamma and alpha on in vitro model of angiogenesis Life and Medical Sciences Cell & Developmental Biology Life and Medical Sciences Cell & Developmental Biology The formation of blood vessels in vivo (angiogenesis) is an important process and is usually initiated in response to injury, tumor growth, or normal tissue development. We have studied the effect of human interferon (IFN) alpha (α) and gamma (γ) on the capillary-like network formation in an in vitro model of angiogenesis using human umbilical vein endothelial cells (HUVEC). When HUVEC cells are plated on Matrigel (reconstituted basement membrane matrix enriched in laminin), a network of capillary like structures (endotubes) rapidly forms. IFN-α enhanced the tube formation in a dose-dependent manner, whereas IFN-γ significantly inhibited the tube formation. In addition, both the enhancement and inhibition of angiogenesis by IFN-α and γ was found to be greater if the cells were pretreated with IFN for 12 hr before plating on the Matrigel. These results suggest that IFN may play an important role in several vascular processes including early stages of wound healing, recanalization of thrombi, tumor growth, metastasis, normal growth, and development. 0021-9541 00219541 Wiley-Blackwell |
| shingle_catch_all_3 | Maheshwari, R. K. Srikantan, V. Bhartiya, D. Kleinman, H. K. Grant, D. S. Differential effects of interferon gamma and alpha on in vitro model of angiogenesis Life and Medical Sciences Cell & Developmental Biology Life and Medical Sciences Cell & Developmental Biology The formation of blood vessels in vivo (angiogenesis) is an important process and is usually initiated in response to injury, tumor growth, or normal tissue development. We have studied the effect of human interferon (IFN) alpha (α) and gamma (γ) on the capillary-like network formation in an in vitro model of angiogenesis using human umbilical vein endothelial cells (HUVEC). When HUVEC cells are plated on Matrigel (reconstituted basement membrane matrix enriched in laminin), a network of capillary like structures (endotubes) rapidly forms. IFN-α enhanced the tube formation in a dose-dependent manner, whereas IFN-γ significantly inhibited the tube formation. In addition, both the enhancement and inhibition of angiogenesis by IFN-α and γ was found to be greater if the cells were pretreated with IFN for 12 hr before plating on the Matrigel. These results suggest that IFN may play an important role in several vascular processes including early stages of wound healing, recanalization of thrombi, tumor growth, metastasis, normal growth, and development. 0021-9541 00219541 Wiley-Blackwell |
| shingle_catch_all_4 | Maheshwari, R. K. Srikantan, V. Bhartiya, D. Kleinman, H. K. Grant, D. S. Differential effects of interferon gamma and alpha on in vitro model of angiogenesis Life and Medical Sciences Cell & Developmental Biology Life and Medical Sciences Cell & Developmental Biology The formation of blood vessels in vivo (angiogenesis) is an important process and is usually initiated in response to injury, tumor growth, or normal tissue development. We have studied the effect of human interferon (IFN) alpha (α) and gamma (γ) on the capillary-like network formation in an in vitro model of angiogenesis using human umbilical vein endothelial cells (HUVEC). When HUVEC cells are plated on Matrigel (reconstituted basement membrane matrix enriched in laminin), a network of capillary like structures (endotubes) rapidly forms. IFN-α enhanced the tube formation in a dose-dependent manner, whereas IFN-γ significantly inhibited the tube formation. In addition, both the enhancement and inhibition of angiogenesis by IFN-α and γ was found to be greater if the cells were pretreated with IFN for 12 hr before plating on the Matrigel. These results suggest that IFN may play an important role in several vascular processes including early stages of wound healing, recanalization of thrombi, tumor growth, metastasis, normal growth, and development. 0021-9541 00219541 Wiley-Blackwell |
| shingle_title_1 | Differential effects of interferon gamma and alpha on in vitro model of angiogenesis |
| shingle_title_2 | Differential effects of interferon gamma and alpha on in vitro model of angiogenesis |
| shingle_title_3 | Differential effects of interferon gamma and alpha on in vitro model of angiogenesis |
| shingle_title_4 | Differential effects of interferon gamma and alpha on in vitro model of angiogenesis |
| sigel_instance_filter | dkfz geomar wilbert ipn albert |
| source_archive | Wiley InterScience Backfile Collection 1832-2000 |
| timestamp | 2024-05-06T10:16:48.479Z |
| titel | Differential effects of interferon gamma and alpha on in vitro model of angiogenesis |
| titel_suche | Differential effects of interferon gamma and alpha on in vitro model of angiogenesis |
| topic | W WW-YZ |
| uid | nat_lic_papers_NLM160803977 |