Loss of EGF binding and cation transport response during differentiation of mouse neuroblastoma cells
Mummery, C. L. ; van der Saag, P. T. ; de Laat, S. W.
New York, N.Y. : Wiley-Blackwell
Published 1983
New York, N.Y. : Wiley-Blackwell
Published 1983
| ISSN: |
0730-2312
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|---|---|
| Keywords: |
neuroblastoma ; differentiation ; EGF ; binding assay ; K+ transport ; Na+ transport ; amiloride ; growth stimulation ; Life and Medical Sciences ; Cell & Developmental Biology
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| Source: |
Wiley InterScience Backfile Collection 1832-2000
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| Topics: |
Biology
Chemistry and Pharmacology
Medicine
|
| Notes: |
Mouse neuroblastoma cells (clone N1E-115) differentiate in culture upon withdrawal of serum growth factors and acquire the characteristics of neurons. We have shown that exponentially growing N1E-115 cells possess functional epidermal growth factor (EGF) receptors but that the capacity for binding EGF and for stimulation of DNA synthesis is lost as the cells differentiate. Furthermore, in exponentially growing cells, EGF induces a rapid increase in amiloride-sensitive Na+ influx, followed by stimulation of the (Na+ -K+)ATPase, indicating that activation of the Na+/H+ exchange mechanism in N1E-115 cells [1] may be induced by EGF. The ionic response is also lost during differentiation, but we have shown that the stimulation of both Na+ and K+ influx is directly proportional to the number of occupied receptors in all cells whether exponentially growing or differentiating, thus only indirectly dependent on the external EGF concentration. The linearity of the relationships indicates that there is no rate-limiting step between EGF binding and the ionic response. Our data would suggest that as neuroblastoma cells differentiate and acquire neuronal properties, their ability to respond to mitogens, both biologically and in the activation of cation transport processes, progressively decreases owing to the loss of the appropriate receptors.
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| Additional Material: |
8 Ill.
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| Type of Medium: |
Electronic Resource
|
| URL: |
| _version_ | 1798297838777532416 |
|---|---|
| addmaterial | 8 Ill. |
| autor | Mummery, C. L. van der Saag, P. T. de Laat, S. W. |
| autorsonst | Mummery, C. L. van der Saag, P. T. de Laat, S. W. |
| book_url | http://dx.doi.org/10.1002/jcb.240210108 |
| datenlieferant | nat_lic_papers |
| hauptsatz | hsatz_simple |
| identnr | NLM159843871 |
| iqvoc_descriptor_keyword | iqvoc_00000124:transport iqvoc_00000124:transport |
| iqvoc_descriptor_title | iqvoc_00000124:transport |
| issn | 0730-2312 |
| journal_name | Journal of Cellular Biochemistry |
| materialart | 1 |
| notes | Mouse neuroblastoma cells (clone N1E-115) differentiate in culture upon withdrawal of serum growth factors and acquire the characteristics of neurons. We have shown that exponentially growing N1E-115 cells possess functional epidermal growth factor (EGF) receptors but that the capacity for binding EGF and for stimulation of DNA synthesis is lost as the cells differentiate. Furthermore, in exponentially growing cells, EGF induces a rapid increase in amiloride-sensitive Na+ influx, followed by stimulation of the (Na+ -K+)ATPase, indicating that activation of the Na+/H+ exchange mechanism in N1E-115 cells [1] may be induced by EGF. The ionic response is also lost during differentiation, but we have shown that the stimulation of both Na+ and K+ influx is directly proportional to the number of occupied receptors in all cells whether exponentially growing or differentiating, thus only indirectly dependent on the external EGF concentration. The linearity of the relationships indicates that there is no rate-limiting step between EGF binding and the ionic response. Our data would suggest that as neuroblastoma cells differentiate and acquire neuronal properties, their ability to respond to mitogens, both biologically and in the activation of cation transport processes, progressively decreases owing to the loss of the appropriate receptors. |
| package_name | Wiley-Blackwell |
| publikationsjahr_anzeige | 1983 |
| publikationsjahr_facette | 1983 |
| publikationsjahr_intervall | 8019:1980-1984 |
| publikationsjahr_sort | 1983 |
| publikationsort | New York, N.Y. |
| publisher | Wiley-Blackwell |
| reference | 21 (1983), S. 63-75 |
| schlagwort | neuroblastoma differentiation EGF binding assay K+ transport Na+ transport amiloride growth stimulation Life and Medical Sciences Cell & Developmental Biology |
| search_space | articles |
| shingle_author_1 | Mummery, C. L. van der Saag, P. T. de Laat, S. W. |
| shingle_author_2 | Mummery, C. L. van der Saag, P. T. de Laat, S. W. |
| shingle_author_3 | Mummery, C. L. van der Saag, P. T. de Laat, S. W. |
| shingle_author_4 | Mummery, C. L. van der Saag, P. T. de Laat, S. W. |
| shingle_catch_all_1 | Mummery, C. L. van der Saag, P. T. de Laat, S. W. Loss of EGF binding and cation transport response during differentiation of mouse neuroblastoma cells neuroblastoma differentiation EGF binding assay K+ transport Na+ transport amiloride growth stimulation Life and Medical Sciences Cell & Developmental Biology neuroblastoma differentiation EGF binding assay K+ transport Na+ transport amiloride growth stimulation Life and Medical Sciences Cell & Developmental Biology Mouse neuroblastoma cells (clone N1E-115) differentiate in culture upon withdrawal of serum growth factors and acquire the characteristics of neurons. We have shown that exponentially growing N1E-115 cells possess functional epidermal growth factor (EGF) receptors but that the capacity for binding EGF and for stimulation of DNA synthesis is lost as the cells differentiate. Furthermore, in exponentially growing cells, EGF induces a rapid increase in amiloride-sensitive Na+ influx, followed by stimulation of the (Na+ -K+)ATPase, indicating that activation of the Na+/H+ exchange mechanism in N1E-115 cells [1] may be induced by EGF. The ionic response is also lost during differentiation, but we have shown that the stimulation of both Na+ and K+ influx is directly proportional to the number of occupied receptors in all cells whether exponentially growing or differentiating, thus only indirectly dependent on the external EGF concentration. The linearity of the relationships indicates that there is no rate-limiting step between EGF binding and the ionic response. Our data would suggest that as neuroblastoma cells differentiate and acquire neuronal properties, their ability to respond to mitogens, both biologically and in the activation of cation transport processes, progressively decreases owing to the loss of the appropriate receptors. 0730-2312 07302312 Wiley-Blackwell |
| shingle_catch_all_2 | Mummery, C. L. van der Saag, P. T. de Laat, S. W. Loss of EGF binding and cation transport response during differentiation of mouse neuroblastoma cells neuroblastoma differentiation EGF binding assay K+ transport Na+ transport amiloride growth stimulation Life and Medical Sciences Cell & Developmental Biology neuroblastoma differentiation EGF binding assay K+ transport Na+ transport amiloride growth stimulation Life and Medical Sciences Cell & Developmental Biology Mouse neuroblastoma cells (clone N1E-115) differentiate in culture upon withdrawal of serum growth factors and acquire the characteristics of neurons. We have shown that exponentially growing N1E-115 cells possess functional epidermal growth factor (EGF) receptors but that the capacity for binding EGF and for stimulation of DNA synthesis is lost as the cells differentiate. Furthermore, in exponentially growing cells, EGF induces a rapid increase in amiloride-sensitive Na+ influx, followed by stimulation of the (Na+ -K+)ATPase, indicating that activation of the Na+/H+ exchange mechanism in N1E-115 cells [1] may be induced by EGF. The ionic response is also lost during differentiation, but we have shown that the stimulation of both Na+ and K+ influx is directly proportional to the number of occupied receptors in all cells whether exponentially growing or differentiating, thus only indirectly dependent on the external EGF concentration. The linearity of the relationships indicates that there is no rate-limiting step between EGF binding and the ionic response. Our data would suggest that as neuroblastoma cells differentiate and acquire neuronal properties, their ability to respond to mitogens, both biologically and in the activation of cation transport processes, progressively decreases owing to the loss of the appropriate receptors. 0730-2312 07302312 Wiley-Blackwell |
| shingle_catch_all_3 | Mummery, C. L. van der Saag, P. T. de Laat, S. W. Loss of EGF binding and cation transport response during differentiation of mouse neuroblastoma cells neuroblastoma differentiation EGF binding assay K+ transport Na+ transport amiloride growth stimulation Life and Medical Sciences Cell & Developmental Biology neuroblastoma differentiation EGF binding assay K+ transport Na+ transport amiloride growth stimulation Life and Medical Sciences Cell & Developmental Biology Mouse neuroblastoma cells (clone N1E-115) differentiate in culture upon withdrawal of serum growth factors and acquire the characteristics of neurons. We have shown that exponentially growing N1E-115 cells possess functional epidermal growth factor (EGF) receptors but that the capacity for binding EGF and for stimulation of DNA synthesis is lost as the cells differentiate. Furthermore, in exponentially growing cells, EGF induces a rapid increase in amiloride-sensitive Na+ influx, followed by stimulation of the (Na+ -K+)ATPase, indicating that activation of the Na+/H+ exchange mechanism in N1E-115 cells [1] may be induced by EGF. The ionic response is also lost during differentiation, but we have shown that the stimulation of both Na+ and K+ influx is directly proportional to the number of occupied receptors in all cells whether exponentially growing or differentiating, thus only indirectly dependent on the external EGF concentration. The linearity of the relationships indicates that there is no rate-limiting step between EGF binding and the ionic response. Our data would suggest that as neuroblastoma cells differentiate and acquire neuronal properties, their ability to respond to mitogens, both biologically and in the activation of cation transport processes, progressively decreases owing to the loss of the appropriate receptors. 0730-2312 07302312 Wiley-Blackwell |
| shingle_catch_all_4 | Mummery, C. L. van der Saag, P. T. de Laat, S. W. Loss of EGF binding and cation transport response during differentiation of mouse neuroblastoma cells neuroblastoma differentiation EGF binding assay K+ transport Na+ transport amiloride growth stimulation Life and Medical Sciences Cell & Developmental Biology neuroblastoma differentiation EGF binding assay K+ transport Na+ transport amiloride growth stimulation Life and Medical Sciences Cell & Developmental Biology Mouse neuroblastoma cells (clone N1E-115) differentiate in culture upon withdrawal of serum growth factors and acquire the characteristics of neurons. We have shown that exponentially growing N1E-115 cells possess functional epidermal growth factor (EGF) receptors but that the capacity for binding EGF and for stimulation of DNA synthesis is lost as the cells differentiate. Furthermore, in exponentially growing cells, EGF induces a rapid increase in amiloride-sensitive Na+ influx, followed by stimulation of the (Na+ -K+)ATPase, indicating that activation of the Na+/H+ exchange mechanism in N1E-115 cells [1] may be induced by EGF. The ionic response is also lost during differentiation, but we have shown that the stimulation of both Na+ and K+ influx is directly proportional to the number of occupied receptors in all cells whether exponentially growing or differentiating, thus only indirectly dependent on the external EGF concentration. The linearity of the relationships indicates that there is no rate-limiting step between EGF binding and the ionic response. Our data would suggest that as neuroblastoma cells differentiate and acquire neuronal properties, their ability to respond to mitogens, both biologically and in the activation of cation transport processes, progressively decreases owing to the loss of the appropriate receptors. 0730-2312 07302312 Wiley-Blackwell |
| shingle_title_1 | Loss of EGF binding and cation transport response during differentiation of mouse neuroblastoma cells |
| shingle_title_2 | Loss of EGF binding and cation transport response during differentiation of mouse neuroblastoma cells |
| shingle_title_3 | Loss of EGF binding and cation transport response during differentiation of mouse neuroblastoma cells |
| shingle_title_4 | Loss of EGF binding and cation transport response during differentiation of mouse neuroblastoma cells |
| sigel_instance_filter | dkfz geomar wilbert ipn albert |
| source_archive | Wiley InterScience Backfile Collection 1832-2000 |
| timestamp | 2024-05-06T10:14:21.933Z |
| titel | Loss of EGF binding and cation transport response during differentiation of mouse neuroblastoma cells |
| titel_suche | Loss of EGF binding and cation transport response during differentiation of mouse neuroblastoma cells |
| topic | W V WW-YZ |
| uid | nat_lic_papers_NLM159843871 |