Comparison of micro-LC and capillary zone electrophoresis for the analysis of thiols
| ISSN: |
0935-6304
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|---|---|
| Keywords: |
Micropacked liquid chromatography ; Capillary zone electrophoresis ; Thiol analysis ; Biological applications ; Chemistry ; Analytical Chemistry and Spectroscopy
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| Source: |
Wiley InterScience Backfile Collection 1832-2000
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| Topics: |
Chemistry and Pharmacology
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| Notes: |
Micro-LC and capillary zone electrophoresis (CZE) are two of the most rapidly developing, miniaturized separation techniques in analytical science. In the present paper, both techniques were comparatively evaluated for the determination of several important thiols derivatized with the fluorogenic reagent SBD-F. Different parameters, such as selectivity, sensitivity, analysis time and efficiency, influence of column length, CZE buffer concentration, and voltage were thus analyzed and discussed. Both techniques are recommended for the analysis of thiols in biological samples.
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| Additional Material: |
5 Ill.
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| Type of Medium: |
Electronic Resource
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| URL: |