Determination of amino acids by gas chromatography using a wide bore capillary column
| ISSN: |
0935-6304
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|---|---|
| Keywords: |
Gas chromatography ; Wide bore glass capillary ; Fused-silica capillary ; Component discrimination ; Butt connectors ; Chemistry ; Analytical Chemistry and Spectroscopy
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| Source: |
Wiley InterScience Backfile Collection 1832-2000
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| Topics: |
Chemistry and Pharmacology
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| Notes: |
A procedure is described in which a wide bore capillary column is used as an alternative to the more traditional packed column for the quantitative analysis of amino acids as their N-heptafluorobutyryl isobutyl ester (HBB) derivatives. The column, installed in a gas chromatograph previously configured for use with a packed column, is shown to give good reproducibility by repeated determination of amino acid response factors (RSD values for all amino acids are below 3%). A number of problems, encountered during the use of this column, are discussed and suitable techniques to overcome them are reported.
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| Additional Material: |
4 Ill.
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| Type of Medium: |
Electronic Resource
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| URL: |