Label-free imaging of amyloid plaques in Alzheimers disease with stimulated Raman scattering microscopy
Ji, M., Arbel, M., Zhang, L., Freudiger, C. W., Hou, S. S., Lin, D., Yang, X., Bacskai, B. J., Xie, X. S.
American Association for the Advancement of Science (AAAS)
Published 2018
American Association for the Advancement of Science (AAAS)
Published 2018
| Publication Date: |
2018-11-17
|
|---|---|
| Publisher: |
American Association for the Advancement of Science (AAAS)
|
| Electronic ISSN: |
2375-2548
|
| Topics: |
Natural Sciences in General
|
| Published by: |
| _version_ | 1839208231010828288 |
|---|---|
| autor | Ji, M., Arbel, M., Zhang, L., Freudiger, C. W., Hou, S. S., Lin, D., Yang, X., Bacskai, B. J., Xie, X. S. |
| beschreibung | One of the key pathological features of Alzheimer’s disease (AD) is the existence of extracellular deposition of amyloid plaques formed with misfolded amyloid-β (Aβ). The conformational change of proteins leads to enriched contents of β sheets, resulting in remarkable changes of vibrational spectra, especially the spectral shifts of the amide I mode. Here, we applied stimulated Raman scattering (SRS) microscopy to image amyloid plaques in the brain tissue of an AD mouse model. We have demonstrated the capability of SRS microscopy as a rapid, label-free imaging modality to differentiate misfolded from normal proteins based on the blue shift (~10 cm –1 ) of amide I SRS spectra. Furthermore, SRS imaging of Aβ plaques was verified by antibody staining of frozen thin sections and fluorescence imaging of fresh tissues. Our method may provide a new approach for studies of AD pathology, as well as other neurodegenerative diseases associated with protein misfolding. |
| citation_standardnr | 6358412 |
| datenlieferant | ipn_articles |
| feed_id | 228416 |
| feed_publisher | American Association for the Advancement of Science (AAAS) |
| feed_publisher_url | http://www.aaas.org/ |
| insertion_date | 2018-11-17 |
| journaleissn | 2375-2548 |
| publikationsjahr_anzeige | 2018 |
| publikationsjahr_facette | 2018 |
| publikationsjahr_intervall | 7984:2015-2019 |
| publikationsjahr_sort | 2018 |
| publisher | American Association for the Advancement of Science (AAAS) |
| quelle | Science Advances |
| relation | http://advances.sciencemag.org/cgi/content/short/4/11/eaat7715?rss=1 |
| search_space | articles |
| shingle_author_1 | Ji, M., Arbel, M., Zhang, L., Freudiger, C. W., Hou, S. S., Lin, D., Yang, X., Bacskai, B. J., Xie, X. S. |
| shingle_author_2 | Ji, M., Arbel, M., Zhang, L., Freudiger, C. W., Hou, S. S., Lin, D., Yang, X., Bacskai, B. J., Xie, X. S. |
| shingle_author_3 | Ji, M., Arbel, M., Zhang, L., Freudiger, C. W., Hou, S. S., Lin, D., Yang, X., Bacskai, B. J., Xie, X. S. |
| shingle_author_4 | Ji, M., Arbel, M., Zhang, L., Freudiger, C. W., Hou, S. S., Lin, D., Yang, X., Bacskai, B. J., Xie, X. S. |
| shingle_catch_all_1 | Label-free imaging of amyloid plaques in Alzheimers disease with stimulated Raman scattering microscopy One of the key pathological features of Alzheimer’s disease (AD) is the existence of extracellular deposition of amyloid plaques formed with misfolded amyloid-β (Aβ). The conformational change of proteins leads to enriched contents of β sheets, resulting in remarkable changes of vibrational spectra, especially the spectral shifts of the amide I mode. Here, we applied stimulated Raman scattering (SRS) microscopy to image amyloid plaques in the brain tissue of an AD mouse model. We have demonstrated the capability of SRS microscopy as a rapid, label-free imaging modality to differentiate misfolded from normal proteins based on the blue shift (~10 cm –1 ) of amide I SRS spectra. Furthermore, SRS imaging of Aβ plaques was verified by antibody staining of frozen thin sections and fluorescence imaging of fresh tissues. Our method may provide a new approach for studies of AD pathology, as well as other neurodegenerative diseases associated with protein misfolding. Ji, M., Arbel, M., Zhang, L., Freudiger, C. W., Hou, S. S., Lin, D., Yang, X., Bacskai, B. J., Xie, X. S. American Association for the Advancement of Science (AAAS) 2375-2548 23752548 |
| shingle_catch_all_2 | Label-free imaging of amyloid plaques in Alzheimers disease with stimulated Raman scattering microscopy One of the key pathological features of Alzheimer’s disease (AD) is the existence of extracellular deposition of amyloid plaques formed with misfolded amyloid-β (Aβ). The conformational change of proteins leads to enriched contents of β sheets, resulting in remarkable changes of vibrational spectra, especially the spectral shifts of the amide I mode. Here, we applied stimulated Raman scattering (SRS) microscopy to image amyloid plaques in the brain tissue of an AD mouse model. We have demonstrated the capability of SRS microscopy as a rapid, label-free imaging modality to differentiate misfolded from normal proteins based on the blue shift (~10 cm –1 ) of amide I SRS spectra. Furthermore, SRS imaging of Aβ plaques was verified by antibody staining of frozen thin sections and fluorescence imaging of fresh tissues. Our method may provide a new approach for studies of AD pathology, as well as other neurodegenerative diseases associated with protein misfolding. Ji, M., Arbel, M., Zhang, L., Freudiger, C. W., Hou, S. S., Lin, D., Yang, X., Bacskai, B. J., Xie, X. S. American Association for the Advancement of Science (AAAS) 2375-2548 23752548 |
| shingle_catch_all_3 | Label-free imaging of amyloid plaques in Alzheimers disease with stimulated Raman scattering microscopy One of the key pathological features of Alzheimer’s disease (AD) is the existence of extracellular deposition of amyloid plaques formed with misfolded amyloid-β (Aβ). The conformational change of proteins leads to enriched contents of β sheets, resulting in remarkable changes of vibrational spectra, especially the spectral shifts of the amide I mode. Here, we applied stimulated Raman scattering (SRS) microscopy to image amyloid plaques in the brain tissue of an AD mouse model. We have demonstrated the capability of SRS microscopy as a rapid, label-free imaging modality to differentiate misfolded from normal proteins based on the blue shift (~10 cm –1 ) of amide I SRS spectra. Furthermore, SRS imaging of Aβ plaques was verified by antibody staining of frozen thin sections and fluorescence imaging of fresh tissues. Our method may provide a new approach for studies of AD pathology, as well as other neurodegenerative diseases associated with protein misfolding. Ji, M., Arbel, M., Zhang, L., Freudiger, C. W., Hou, S. S., Lin, D., Yang, X., Bacskai, B. J., Xie, X. S. American Association for the Advancement of Science (AAAS) 2375-2548 23752548 |
| shingle_catch_all_4 | Label-free imaging of amyloid plaques in Alzheimers disease with stimulated Raman scattering microscopy One of the key pathological features of Alzheimer’s disease (AD) is the existence of extracellular deposition of amyloid plaques formed with misfolded amyloid-β (Aβ). The conformational change of proteins leads to enriched contents of β sheets, resulting in remarkable changes of vibrational spectra, especially the spectral shifts of the amide I mode. Here, we applied stimulated Raman scattering (SRS) microscopy to image amyloid plaques in the brain tissue of an AD mouse model. We have demonstrated the capability of SRS microscopy as a rapid, label-free imaging modality to differentiate misfolded from normal proteins based on the blue shift (~10 cm –1 ) of amide I SRS spectra. Furthermore, SRS imaging of Aβ plaques was verified by antibody staining of frozen thin sections and fluorescence imaging of fresh tissues. Our method may provide a new approach for studies of AD pathology, as well as other neurodegenerative diseases associated with protein misfolding. Ji, M., Arbel, M., Zhang, L., Freudiger, C. W., Hou, S. S., Lin, D., Yang, X., Bacskai, B. J., Xie, X. S. American Association for the Advancement of Science (AAAS) 2375-2548 23752548 |
| shingle_title_1 | Label-free imaging of amyloid plaques in Alzheimers disease with stimulated Raman scattering microscopy |
| shingle_title_2 | Label-free imaging of amyloid plaques in Alzheimers disease with stimulated Raman scattering microscopy |
| shingle_title_3 | Label-free imaging of amyloid plaques in Alzheimers disease with stimulated Raman scattering microscopy |
| shingle_title_4 | Label-free imaging of amyloid plaques in Alzheimers disease with stimulated Raman scattering microscopy |
| timestamp | 2025-07-31T23:47:31.812Z |
| titel | Label-free imaging of amyloid plaques in Alzheimers disease with stimulated Raman scattering microscopy |
| titel_suche | Label-free imaging of amyloid plaques in Alzheimers disease with stimulated Raman scattering microscopy |
| topic | TA-TD |
| uid | ipn_articles_6358412 |