Single-Cell Analyses of Prostate Cancer Liquid Biopsies Acquired by Apheresis

Publication Date:
2018-11-16
Publisher:
The American Association for Cancer Research (AACR)
Print ISSN:
1078-0432
Electronic ISSN:
1557-3265
Topics:
Medicine
Published by:
http://www.aacr.org/
_version_ 1836399087116615680
autor Lambros, M. B., Seed, G., Sumanasuriya, S., Gil, V., Crespo, M., Fontes, M., Chandler, R., Mehra, N., Fowler, G., Ebbs, B., Flohr, P., Miranda, S., Yuan, W., Mackay, A., Ferreira, A., Pereira, R., Bertan, C., Figueiredo, I., Riisnaes, R., Rodrigues, D. N., Sharp, A., Goodall, J., Boysen, G., Carreira, S., Bianchini, D., Rescigno, P., Zafeiriou, Z., Hunt, J., Moloney, D., Hamilton, L., Neves, R. P., Swennenhuis, J., Andree, K., Stoecklein, N. H., Terstappen, L. W. M. M., de Bono, J. S.
beschreibung Purpose: Circulating tumor cells (CTCs) have clinical relevance, but their study has been limited by their low frequency. Experimental Design: We evaluated liquid biopsies by apheresis to increase CTC yield from patients suffering from metastatic prostate cancer, allow precise gene copy-number calls, and study disease heterogeneity. Results: Apheresis was well tolerated and allowed the separation of large numbers of CTCs; the average CTC yield from 7.5 mL of peripheral blood was 167 CTCs, whereas the average CTC yield per apheresis (mean volume: 59.5 mL) was 12,546 CTCs. Purified single CTCs could be isolated from apheresis product by FACS sorting; copy-number aberration (CNA) profiles of 185 single CTCs from 14 patients revealed the genomic landscape of lethal prostate cancer and identified complex intrapatient, intercell, genomic heterogeneity missed on bulk biopsy analyses. Conclusions: Apheresis facilitated the capture of large numbers of CTCs noninvasively with minimal morbidity and allowed the deconvolution of intrapatient heterogeneity and clonal evolution. Clin Cancer Res; 24(22); 5635–44. ©2018 AACR .
citation_standardnr 6357397
datenlieferant ipn_articles
feed_id 9363
feed_publisher The American Association for Cancer Research (AACR)
feed_publisher_url http://www.aacr.org/
insertion_date 2018-11-16
journaleissn 1557-3265
journalissn 1078-0432
publikationsjahr_anzeige 2018
publikationsjahr_facette 2018
publikationsjahr_intervall 7984:2015-2019
publikationsjahr_sort 2018
publisher The American Association for Cancer Research (AACR)
quelle Clinical Cancer Research
relation http://clincancerres.aacrjournals.org/cgi/content/short/24/22/5635?rss=1
search_space articles
shingle_author_1 Lambros, M. B., Seed, G., Sumanasuriya, S., Gil, V., Crespo, M., Fontes, M., Chandler, R., Mehra, N., Fowler, G., Ebbs, B., Flohr, P., Miranda, S., Yuan, W., Mackay, A., Ferreira, A., Pereira, R., Bertan, C., Figueiredo, I., Riisnaes, R., Rodrigues, D. N., Sharp, A., Goodall, J., Boysen, G., Carreira, S., Bianchini, D., Rescigno, P., Zafeiriou, Z., Hunt, J., Moloney, D., Hamilton, L., Neves, R. P., Swennenhuis, J., Andree, K., Stoecklein, N. H., Terstappen, L. W. M. M., de Bono, J. S.
shingle_author_2 Lambros, M. B., Seed, G., Sumanasuriya, S., Gil, V., Crespo, M., Fontes, M., Chandler, R., Mehra, N., Fowler, G., Ebbs, B., Flohr, P., Miranda, S., Yuan, W., Mackay, A., Ferreira, A., Pereira, R., Bertan, C., Figueiredo, I., Riisnaes, R., Rodrigues, D. N., Sharp, A., Goodall, J., Boysen, G., Carreira, S., Bianchini, D., Rescigno, P., Zafeiriou, Z., Hunt, J., Moloney, D., Hamilton, L., Neves, R. P., Swennenhuis, J., Andree, K., Stoecklein, N. H., Terstappen, L. W. M. M., de Bono, J. S.
shingle_author_3 Lambros, M. B., Seed, G., Sumanasuriya, S., Gil, V., Crespo, M., Fontes, M., Chandler, R., Mehra, N., Fowler, G., Ebbs, B., Flohr, P., Miranda, S., Yuan, W., Mackay, A., Ferreira, A., Pereira, R., Bertan, C., Figueiredo, I., Riisnaes, R., Rodrigues, D. N., Sharp, A., Goodall, J., Boysen, G., Carreira, S., Bianchini, D., Rescigno, P., Zafeiriou, Z., Hunt, J., Moloney, D., Hamilton, L., Neves, R. P., Swennenhuis, J., Andree, K., Stoecklein, N. H., Terstappen, L. W. M. M., de Bono, J. S.
shingle_author_4 Lambros, M. B., Seed, G., Sumanasuriya, S., Gil, V., Crespo, M., Fontes, M., Chandler, R., Mehra, N., Fowler, G., Ebbs, B., Flohr, P., Miranda, S., Yuan, W., Mackay, A., Ferreira, A., Pereira, R., Bertan, C., Figueiredo, I., Riisnaes, R., Rodrigues, D. N., Sharp, A., Goodall, J., Boysen, G., Carreira, S., Bianchini, D., Rescigno, P., Zafeiriou, Z., Hunt, J., Moloney, D., Hamilton, L., Neves, R. P., Swennenhuis, J., Andree, K., Stoecklein, N. H., Terstappen, L. W. M. M., de Bono, J. S.
shingle_catch_all_1 Single-Cell Analyses of Prostate Cancer Liquid Biopsies Acquired by Apheresis
Purpose: Circulating tumor cells (CTCs) have clinical relevance, but their study has been limited by their low frequency. Experimental Design: We evaluated liquid biopsies by apheresis to increase CTC yield from patients suffering from metastatic prostate cancer, allow precise gene copy-number calls, and study disease heterogeneity. Results: Apheresis was well tolerated and allowed the separation of large numbers of CTCs; the average CTC yield from 7.5 mL of peripheral blood was 167 CTCs, whereas the average CTC yield per apheresis (mean volume: 59.5 mL) was 12,546 CTCs. Purified single CTCs could be isolated from apheresis product by FACS sorting; copy-number aberration (CNA) profiles of 185 single CTCs from 14 patients revealed the genomic landscape of lethal prostate cancer and identified complex intrapatient, intercell, genomic heterogeneity missed on bulk biopsy analyses. Conclusions: Apheresis facilitated the capture of large numbers of CTCs noninvasively with minimal morbidity and allowed the deconvolution of intrapatient heterogeneity and clonal evolution. Clin Cancer Res; 24(22); 5635–44. ©2018 AACR .
Lambros, M. B., Seed, G., Sumanasuriya, S., Gil, V., Crespo, M., Fontes, M., Chandler, R., Mehra, N., Fowler, G., Ebbs, B., Flohr, P., Miranda, S., Yuan, W., Mackay, A., Ferreira, A., Pereira, R., Bertan, C., Figueiredo, I., Riisnaes, R., Rodrigues, D. N., Sharp, A., Goodall, J., Boysen, G., Carreira, S., Bianchini, D., Rescigno, P., Zafeiriou, Z., Hunt, J., Moloney, D., Hamilton, L., Neves, R. P., Swennenhuis, J., Andree, K., Stoecklein, N. H., Terstappen, L. W. M. M., de Bono, J. S.
The American Association for Cancer Research (AACR)
1078-0432
10780432
1557-3265
15573265
shingle_catch_all_2 Single-Cell Analyses of Prostate Cancer Liquid Biopsies Acquired by Apheresis
Purpose: Circulating tumor cells (CTCs) have clinical relevance, but their study has been limited by their low frequency. Experimental Design: We evaluated liquid biopsies by apheresis to increase CTC yield from patients suffering from metastatic prostate cancer, allow precise gene copy-number calls, and study disease heterogeneity. Results: Apheresis was well tolerated and allowed the separation of large numbers of CTCs; the average CTC yield from 7.5 mL of peripheral blood was 167 CTCs, whereas the average CTC yield per apheresis (mean volume: 59.5 mL) was 12,546 CTCs. Purified single CTCs could be isolated from apheresis product by FACS sorting; copy-number aberration (CNA) profiles of 185 single CTCs from 14 patients revealed the genomic landscape of lethal prostate cancer and identified complex intrapatient, intercell, genomic heterogeneity missed on bulk biopsy analyses. Conclusions: Apheresis facilitated the capture of large numbers of CTCs noninvasively with minimal morbidity and allowed the deconvolution of intrapatient heterogeneity and clonal evolution. Clin Cancer Res; 24(22); 5635–44. ©2018 AACR .
Lambros, M. B., Seed, G., Sumanasuriya, S., Gil, V., Crespo, M., Fontes, M., Chandler, R., Mehra, N., Fowler, G., Ebbs, B., Flohr, P., Miranda, S., Yuan, W., Mackay, A., Ferreira, A., Pereira, R., Bertan, C., Figueiredo, I., Riisnaes, R., Rodrigues, D. N., Sharp, A., Goodall, J., Boysen, G., Carreira, S., Bianchini, D., Rescigno, P., Zafeiriou, Z., Hunt, J., Moloney, D., Hamilton, L., Neves, R. P., Swennenhuis, J., Andree, K., Stoecklein, N. H., Terstappen, L. W. M. M., de Bono, J. S.
The American Association for Cancer Research (AACR)
1078-0432
10780432
1557-3265
15573265
shingle_catch_all_3 Single-Cell Analyses of Prostate Cancer Liquid Biopsies Acquired by Apheresis
Purpose: Circulating tumor cells (CTCs) have clinical relevance, but their study has been limited by their low frequency. Experimental Design: We evaluated liquid biopsies by apheresis to increase CTC yield from patients suffering from metastatic prostate cancer, allow precise gene copy-number calls, and study disease heterogeneity. Results: Apheresis was well tolerated and allowed the separation of large numbers of CTCs; the average CTC yield from 7.5 mL of peripheral blood was 167 CTCs, whereas the average CTC yield per apheresis (mean volume: 59.5 mL) was 12,546 CTCs. Purified single CTCs could be isolated from apheresis product by FACS sorting; copy-number aberration (CNA) profiles of 185 single CTCs from 14 patients revealed the genomic landscape of lethal prostate cancer and identified complex intrapatient, intercell, genomic heterogeneity missed on bulk biopsy analyses. Conclusions: Apheresis facilitated the capture of large numbers of CTCs noninvasively with minimal morbidity and allowed the deconvolution of intrapatient heterogeneity and clonal evolution. Clin Cancer Res; 24(22); 5635–44. ©2018 AACR .
Lambros, M. B., Seed, G., Sumanasuriya, S., Gil, V., Crespo, M., Fontes, M., Chandler, R., Mehra, N., Fowler, G., Ebbs, B., Flohr, P., Miranda, S., Yuan, W., Mackay, A., Ferreira, A., Pereira, R., Bertan, C., Figueiredo, I., Riisnaes, R., Rodrigues, D. N., Sharp, A., Goodall, J., Boysen, G., Carreira, S., Bianchini, D., Rescigno, P., Zafeiriou, Z., Hunt, J., Moloney, D., Hamilton, L., Neves, R. P., Swennenhuis, J., Andree, K., Stoecklein, N. H., Terstappen, L. W. M. M., de Bono, J. S.
The American Association for Cancer Research (AACR)
1078-0432
10780432
1557-3265
15573265
shingle_catch_all_4 Single-Cell Analyses of Prostate Cancer Liquid Biopsies Acquired by Apheresis
Purpose: Circulating tumor cells (CTCs) have clinical relevance, but their study has been limited by their low frequency. Experimental Design: We evaluated liquid biopsies by apheresis to increase CTC yield from patients suffering from metastatic prostate cancer, allow precise gene copy-number calls, and study disease heterogeneity. Results: Apheresis was well tolerated and allowed the separation of large numbers of CTCs; the average CTC yield from 7.5 mL of peripheral blood was 167 CTCs, whereas the average CTC yield per apheresis (mean volume: 59.5 mL) was 12,546 CTCs. Purified single CTCs could be isolated from apheresis product by FACS sorting; copy-number aberration (CNA) profiles of 185 single CTCs from 14 patients revealed the genomic landscape of lethal prostate cancer and identified complex intrapatient, intercell, genomic heterogeneity missed on bulk biopsy analyses. Conclusions: Apheresis facilitated the capture of large numbers of CTCs noninvasively with minimal morbidity and allowed the deconvolution of intrapatient heterogeneity and clonal evolution. Clin Cancer Res; 24(22); 5635–44. ©2018 AACR .
Lambros, M. B., Seed, G., Sumanasuriya, S., Gil, V., Crespo, M., Fontes, M., Chandler, R., Mehra, N., Fowler, G., Ebbs, B., Flohr, P., Miranda, S., Yuan, W., Mackay, A., Ferreira, A., Pereira, R., Bertan, C., Figueiredo, I., Riisnaes, R., Rodrigues, D. N., Sharp, A., Goodall, J., Boysen, G., Carreira, S., Bianchini, D., Rescigno, P., Zafeiriou, Z., Hunt, J., Moloney, D., Hamilton, L., Neves, R. P., Swennenhuis, J., Andree, K., Stoecklein, N. H., Terstappen, L. W. M. M., de Bono, J. S.
The American Association for Cancer Research (AACR)
1078-0432
10780432
1557-3265
15573265
shingle_title_1 Single-Cell Analyses of Prostate Cancer Liquid Biopsies Acquired by Apheresis
shingle_title_2 Single-Cell Analyses of Prostate Cancer Liquid Biopsies Acquired by Apheresis
shingle_title_3 Single-Cell Analyses of Prostate Cancer Liquid Biopsies Acquired by Apheresis
shingle_title_4 Single-Cell Analyses of Prostate Cancer Liquid Biopsies Acquired by Apheresis
timestamp 2025-06-30T23:37:23.576Z
titel Single-Cell Analyses of Prostate Cancer Liquid Biopsies Acquired by Apheresis
titel_suche Single-Cell Analyses of Prostate Cancer Liquid Biopsies Acquired by Apheresis
topic WW-YZ
uid ipn_articles_6357397