Signatures of Insecticide Selection in the Genome of Drosophila melanogaster

Publication Date:
2018-11-07
Publisher:
Genetics Society of America (GSA)
Electronic ISSN:
2160-1836
Topics:
Biology
Published by:
http://www.genetics-gsa.org/
_version_ 1836399081086255106
autor Duneau, D., Sun, H., Revah, J., San Miguel, K., Kunerth, H. D., Caldas, I. V., Messer, P. W., Scott, J. G., Buchon, N.
beschreibung Resistance to insecticides has evolved in multiple insect species, leading to increased application rates and even control failures. Understanding the genetic basis of insecticide resistance is fundamental for mitigating its impact on crop production and disease control. We performed a GWAS approach with the Drosophila Genetic Reference Panel (DGRP) to identify the mutations involved in resistance to two widely used classes of insecticides: organophosphates (OPs, parathion) and pyrethroids (deltamethrin). Most variation in parathion resistance was associated with mutations in the target gene Ace , while most variation in deltamethrin resistance was associated with mutations in Cyp6a23 , a gene encoding a detoxification enzyme never previously associated with resistance. A "nested GWAS" further revealed the contribution of other loci: Dscam1 and trpl were implicated in resistance to parathion, but only in lines lacking Wolbachia . Cyp6a17 , the paralogous gene of Cyp6a23 , and CG7627 , an ATP-binding cassette transporter, were implicated in deltamethrin resistance. We observed signatures of recent selective sweeps at all of these resistance loci and confirmed that the soft sweep at Ace is indeed driven by the identified resistance mutations. Analysis of allele frequencies in additional population samples revealed that most resistance mutations are segregating across the globe, but that frequencies can vary substantially among populations. Altogether, our data reveal that the widely used OP and pyrethroid insecticides imposed a strong selection pressure on natural insect populations. However, it remains unclear why, in Drosophila , resistance evolved due to changes in the target site for OPs, but due to a detoxification enzyme for pyrethroids.
citation_standardnr 6353713
datenlieferant ipn_articles
feed_id 169615
feed_publisher Genetics Society of America (GSA)
feed_publisher_url http://www.genetics-gsa.org/
insertion_date 2018-11-07
journaleissn 2160-1836
publikationsjahr_anzeige 2018
publikationsjahr_facette 2018
publikationsjahr_intervall 7984:2015-2019
publikationsjahr_sort 2018
publisher Genetics Society of America (GSA)
quelle G3: Genes, Genomes, Genetics
relation http://www.g3journal.org/cgi/content/short/8/11/3469?rss=1
search_space articles
shingle_author_1 Duneau, D., Sun, H., Revah, J., San Miguel, K., Kunerth, H. D., Caldas, I. V., Messer, P. W., Scott, J. G., Buchon, N.
shingle_author_2 Duneau, D., Sun, H., Revah, J., San Miguel, K., Kunerth, H. D., Caldas, I. V., Messer, P. W., Scott, J. G., Buchon, N.
shingle_author_3 Duneau, D., Sun, H., Revah, J., San Miguel, K., Kunerth, H. D., Caldas, I. V., Messer, P. W., Scott, J. G., Buchon, N.
shingle_author_4 Duneau, D., Sun, H., Revah, J., San Miguel, K., Kunerth, H. D., Caldas, I. V., Messer, P. W., Scott, J. G., Buchon, N.
shingle_catch_all_1 Signatures of Insecticide Selection in the Genome of Drosophila melanogaster
Resistance to insecticides has evolved in multiple insect species, leading to increased application rates and even control failures. Understanding the genetic basis of insecticide resistance is fundamental for mitigating its impact on crop production and disease control. We performed a GWAS approach with the Drosophila Genetic Reference Panel (DGRP) to identify the mutations involved in resistance to two widely used classes of insecticides: organophosphates (OPs, parathion) and pyrethroids (deltamethrin). Most variation in parathion resistance was associated with mutations in the target gene Ace , while most variation in deltamethrin resistance was associated with mutations in Cyp6a23 , a gene encoding a detoxification enzyme never previously associated with resistance. A "nested GWAS" further revealed the contribution of other loci: Dscam1 and trpl were implicated in resistance to parathion, but only in lines lacking Wolbachia . Cyp6a17 , the paralogous gene of Cyp6a23 , and CG7627 , an ATP-binding cassette transporter, were implicated in deltamethrin resistance. We observed signatures of recent selective sweeps at all of these resistance loci and confirmed that the soft sweep at Ace is indeed driven by the identified resistance mutations. Analysis of allele frequencies in additional population samples revealed that most resistance mutations are segregating across the globe, but that frequencies can vary substantially among populations. Altogether, our data reveal that the widely used OP and pyrethroid insecticides imposed a strong selection pressure on natural insect populations. However, it remains unclear why, in Drosophila , resistance evolved due to changes in the target site for OPs, but due to a detoxification enzyme for pyrethroids.
Duneau, D., Sun, H., Revah, J., San Miguel, K., Kunerth, H. D., Caldas, I. V., Messer, P. W., Scott, J. G., Buchon, N.
Genetics Society of America (GSA)
2160-1836
21601836
shingle_catch_all_2 Signatures of Insecticide Selection in the Genome of Drosophila melanogaster
Resistance to insecticides has evolved in multiple insect species, leading to increased application rates and even control failures. Understanding the genetic basis of insecticide resistance is fundamental for mitigating its impact on crop production and disease control. We performed a GWAS approach with the Drosophila Genetic Reference Panel (DGRP) to identify the mutations involved in resistance to two widely used classes of insecticides: organophosphates (OPs, parathion) and pyrethroids (deltamethrin). Most variation in parathion resistance was associated with mutations in the target gene Ace , while most variation in deltamethrin resistance was associated with mutations in Cyp6a23 , a gene encoding a detoxification enzyme never previously associated with resistance. A "nested GWAS" further revealed the contribution of other loci: Dscam1 and trpl were implicated in resistance to parathion, but only in lines lacking Wolbachia . Cyp6a17 , the paralogous gene of Cyp6a23 , and CG7627 , an ATP-binding cassette transporter, were implicated in deltamethrin resistance. We observed signatures of recent selective sweeps at all of these resistance loci and confirmed that the soft sweep at Ace is indeed driven by the identified resistance mutations. Analysis of allele frequencies in additional population samples revealed that most resistance mutations are segregating across the globe, but that frequencies can vary substantially among populations. Altogether, our data reveal that the widely used OP and pyrethroid insecticides imposed a strong selection pressure on natural insect populations. However, it remains unclear why, in Drosophila , resistance evolved due to changes in the target site for OPs, but due to a detoxification enzyme for pyrethroids.
Duneau, D., Sun, H., Revah, J., San Miguel, K., Kunerth, H. D., Caldas, I. V., Messer, P. W., Scott, J. G., Buchon, N.
Genetics Society of America (GSA)
2160-1836
21601836
shingle_catch_all_3 Signatures of Insecticide Selection in the Genome of Drosophila melanogaster
Resistance to insecticides has evolved in multiple insect species, leading to increased application rates and even control failures. Understanding the genetic basis of insecticide resistance is fundamental for mitigating its impact on crop production and disease control. We performed a GWAS approach with the Drosophila Genetic Reference Panel (DGRP) to identify the mutations involved in resistance to two widely used classes of insecticides: organophosphates (OPs, parathion) and pyrethroids (deltamethrin). Most variation in parathion resistance was associated with mutations in the target gene Ace , while most variation in deltamethrin resistance was associated with mutations in Cyp6a23 , a gene encoding a detoxification enzyme never previously associated with resistance. A "nested GWAS" further revealed the contribution of other loci: Dscam1 and trpl were implicated in resistance to parathion, but only in lines lacking Wolbachia . Cyp6a17 , the paralogous gene of Cyp6a23 , and CG7627 , an ATP-binding cassette transporter, were implicated in deltamethrin resistance. We observed signatures of recent selective sweeps at all of these resistance loci and confirmed that the soft sweep at Ace is indeed driven by the identified resistance mutations. Analysis of allele frequencies in additional population samples revealed that most resistance mutations are segregating across the globe, but that frequencies can vary substantially among populations. Altogether, our data reveal that the widely used OP and pyrethroid insecticides imposed a strong selection pressure on natural insect populations. However, it remains unclear why, in Drosophila , resistance evolved due to changes in the target site for OPs, but due to a detoxification enzyme for pyrethroids.
Duneau, D., Sun, H., Revah, J., San Miguel, K., Kunerth, H. D., Caldas, I. V., Messer, P. W., Scott, J. G., Buchon, N.
Genetics Society of America (GSA)
2160-1836
21601836
shingle_catch_all_4 Signatures of Insecticide Selection in the Genome of Drosophila melanogaster
Resistance to insecticides has evolved in multiple insect species, leading to increased application rates and even control failures. Understanding the genetic basis of insecticide resistance is fundamental for mitigating its impact on crop production and disease control. We performed a GWAS approach with the Drosophila Genetic Reference Panel (DGRP) to identify the mutations involved in resistance to two widely used classes of insecticides: organophosphates (OPs, parathion) and pyrethroids (deltamethrin). Most variation in parathion resistance was associated with mutations in the target gene Ace , while most variation in deltamethrin resistance was associated with mutations in Cyp6a23 , a gene encoding a detoxification enzyme never previously associated with resistance. A "nested GWAS" further revealed the contribution of other loci: Dscam1 and trpl were implicated in resistance to parathion, but only in lines lacking Wolbachia . Cyp6a17 , the paralogous gene of Cyp6a23 , and CG7627 , an ATP-binding cassette transporter, were implicated in deltamethrin resistance. We observed signatures of recent selective sweeps at all of these resistance loci and confirmed that the soft sweep at Ace is indeed driven by the identified resistance mutations. Analysis of allele frequencies in additional population samples revealed that most resistance mutations are segregating across the globe, but that frequencies can vary substantially among populations. Altogether, our data reveal that the widely used OP and pyrethroid insecticides imposed a strong selection pressure on natural insect populations. However, it remains unclear why, in Drosophila , resistance evolved due to changes in the target site for OPs, but due to a detoxification enzyme for pyrethroids.
Duneau, D., Sun, H., Revah, J., San Miguel, K., Kunerth, H. D., Caldas, I. V., Messer, P. W., Scott, J. G., Buchon, N.
Genetics Society of America (GSA)
2160-1836
21601836
shingle_title_1 Signatures of Insecticide Selection in the Genome of Drosophila melanogaster
shingle_title_2 Signatures of Insecticide Selection in the Genome of Drosophila melanogaster
shingle_title_3 Signatures of Insecticide Selection in the Genome of Drosophila melanogaster
shingle_title_4 Signatures of Insecticide Selection in the Genome of Drosophila melanogaster
timestamp 2025-06-30T23:37:18.229Z
titel Signatures of Insecticide Selection in the Genome of Drosophila melanogaster
titel_suche Signatures of Insecticide Selection in the Genome of Drosophila melanogaster
topic W
uid ipn_articles_6353713