Pleiotropic Impacts of Macrophage and Microglial Deficiency on Development in Rats with Targeted Mutation of the Csf1r Locus [IMMUNE SYSTEM DEVELOPMENT]

Publication Date:
2018-10-23
Publisher:
The American Association of Immunologists (AAI)
Print ISSN:
0022-1767
Electronic ISSN:
1550-6606
Topics:
Medicine
Published by:
http://www.aai.org/
_version_ 1836399071913312258
autor Pridans, C., Raper, A., Davis, G. M., Alves, J., Sauter, K. A., Lefevre, L., Regan, T., Meek, S., Sutherland, L., Thomson, A. J., Clohisey, S., Bush, S. J., Rojo, R., Lisowski, Z. M., Wallace, R., Grabert, K., Upton, K. R., Tsai, Y. T., Brown, D., Smith, L. B., Summers, K. M., Mabbott, N. A., Piccardo, P., Cheeseman, M. T., Burdon, T., Hume, D. A.
beschreibung We have produced Csf1r -deficient rats by homologous recombination in embryonic stem cells. Consistent with the role of Csf1r in macrophage differentiation, there was a loss of peripheral blood monocytes, microglia in the brain, epidermal Langerhans cells, splenic marginal zone macrophages, bone-associated macrophages and osteoclasts, and peritoneal macrophages. Macrophages of splenic red pulp, liver, lung, and gut were less affected. The pleiotropic impacts of the loss of macrophages on development of multiple organ systems in rats were distinct from those reported in mice. Csf1r –/– rats survived well into adulthood with postnatal growth retardation, distinct skeletal and bone marrow abnormalities, infertility, and loss of visceral adipose tissue. Gene expression analysis in spleen revealed selective loss of transcripts associated with the marginal zone and, in brain regions, the loss of known and candidate novel microglia-associated transcripts. Despite the complete absence of microglia, there was little overt phenotype in brain, aside from reduced myelination and increased expression of dopamine receptor-associated transcripts in striatum. The results highlight the redundant and nonredundant functions of CSF1R signaling and of macrophages in development, organogenesis, and homeostasis.
citation_standardnr 6347309
datenlieferant ipn_articles
feed_id 333
feed_publisher The American Association of Immunologists (AAI)
feed_publisher_url http://www.aai.org/
insertion_date 2018-10-23
journaleissn 1550-6606
journalissn 0022-1767
publikationsjahr_anzeige 2018
publikationsjahr_facette 2018
publikationsjahr_intervall 7984:2015-2019
publikationsjahr_sort 2018
publisher The American Association of Immunologists (AAI)
quelle Journal of Immunology
relation http://www.jimmunol.org/cgi/content/short/201/9/2683?rss=1
search_space articles
shingle_author_1 Pridans, C., Raper, A., Davis, G. M., Alves, J., Sauter, K. A., Lefevre, L., Regan, T., Meek, S., Sutherland, L., Thomson, A. J., Clohisey, S., Bush, S. J., Rojo, R., Lisowski, Z. M., Wallace, R., Grabert, K., Upton, K. R., Tsai, Y. T., Brown, D., Smith, L. B., Summers, K. M., Mabbott, N. A., Piccardo, P., Cheeseman, M. T., Burdon, T., Hume, D. A.
shingle_author_2 Pridans, C., Raper, A., Davis, G. M., Alves, J., Sauter, K. A., Lefevre, L., Regan, T., Meek, S., Sutherland, L., Thomson, A. J., Clohisey, S., Bush, S. J., Rojo, R., Lisowski, Z. M., Wallace, R., Grabert, K., Upton, K. R., Tsai, Y. T., Brown, D., Smith, L. B., Summers, K. M., Mabbott, N. A., Piccardo, P., Cheeseman, M. T., Burdon, T., Hume, D. A.
shingle_author_3 Pridans, C., Raper, A., Davis, G. M., Alves, J., Sauter, K. A., Lefevre, L., Regan, T., Meek, S., Sutherland, L., Thomson, A. J., Clohisey, S., Bush, S. J., Rojo, R., Lisowski, Z. M., Wallace, R., Grabert, K., Upton, K. R., Tsai, Y. T., Brown, D., Smith, L. B., Summers, K. M., Mabbott, N. A., Piccardo, P., Cheeseman, M. T., Burdon, T., Hume, D. A.
shingle_author_4 Pridans, C., Raper, A., Davis, G. M., Alves, J., Sauter, K. A., Lefevre, L., Regan, T., Meek, S., Sutherland, L., Thomson, A. J., Clohisey, S., Bush, S. J., Rojo, R., Lisowski, Z. M., Wallace, R., Grabert, K., Upton, K. R., Tsai, Y. T., Brown, D., Smith, L. B., Summers, K. M., Mabbott, N. A., Piccardo, P., Cheeseman, M. T., Burdon, T., Hume, D. A.
shingle_catch_all_1 Pleiotropic Impacts of Macrophage and Microglial Deficiency on Development in Rats with Targeted Mutation of the Csf1r Locus [IMMUNE SYSTEM DEVELOPMENT]
We have produced Csf1r -deficient rats by homologous recombination in embryonic stem cells. Consistent with the role of Csf1r in macrophage differentiation, there was a loss of peripheral blood monocytes, microglia in the brain, epidermal Langerhans cells, splenic marginal zone macrophages, bone-associated macrophages and osteoclasts, and peritoneal macrophages. Macrophages of splenic red pulp, liver, lung, and gut were less affected. The pleiotropic impacts of the loss of macrophages on development of multiple organ systems in rats were distinct from those reported in mice. Csf1r –/– rats survived well into adulthood with postnatal growth retardation, distinct skeletal and bone marrow abnormalities, infertility, and loss of visceral adipose tissue. Gene expression analysis in spleen revealed selective loss of transcripts associated with the marginal zone and, in brain regions, the loss of known and candidate novel microglia-associated transcripts. Despite the complete absence of microglia, there was little overt phenotype in brain, aside from reduced myelination and increased expression of dopamine receptor-associated transcripts in striatum. The results highlight the redundant and nonredundant functions of CSF1R signaling and of macrophages in development, organogenesis, and homeostasis.
Pridans, C., Raper, A., Davis, G. M., Alves, J., Sauter, K. A., Lefevre, L., Regan, T., Meek, S., Sutherland, L., Thomson, A. J., Clohisey, S., Bush, S. J., Rojo, R., Lisowski, Z. M., Wallace, R., Grabert, K., Upton, K. R., Tsai, Y. T., Brown, D., Smith, L. B., Summers, K. M., Mabbott, N. A., Piccardo, P., Cheeseman, M. T., Burdon, T., Hume, D. A.
The American Association of Immunologists (AAI)
0022-1767
00221767
1550-6606
15506606
shingle_catch_all_2 Pleiotropic Impacts of Macrophage and Microglial Deficiency on Development in Rats with Targeted Mutation of the Csf1r Locus [IMMUNE SYSTEM DEVELOPMENT]
We have produced Csf1r -deficient rats by homologous recombination in embryonic stem cells. Consistent with the role of Csf1r in macrophage differentiation, there was a loss of peripheral blood monocytes, microglia in the brain, epidermal Langerhans cells, splenic marginal zone macrophages, bone-associated macrophages and osteoclasts, and peritoneal macrophages. Macrophages of splenic red pulp, liver, lung, and gut were less affected. The pleiotropic impacts of the loss of macrophages on development of multiple organ systems in rats were distinct from those reported in mice. Csf1r –/– rats survived well into adulthood with postnatal growth retardation, distinct skeletal and bone marrow abnormalities, infertility, and loss of visceral adipose tissue. Gene expression analysis in spleen revealed selective loss of transcripts associated with the marginal zone and, in brain regions, the loss of known and candidate novel microglia-associated transcripts. Despite the complete absence of microglia, there was little overt phenotype in brain, aside from reduced myelination and increased expression of dopamine receptor-associated transcripts in striatum. The results highlight the redundant and nonredundant functions of CSF1R signaling and of macrophages in development, organogenesis, and homeostasis.
Pridans, C., Raper, A., Davis, G. M., Alves, J., Sauter, K. A., Lefevre, L., Regan, T., Meek, S., Sutherland, L., Thomson, A. J., Clohisey, S., Bush, S. J., Rojo, R., Lisowski, Z. M., Wallace, R., Grabert, K., Upton, K. R., Tsai, Y. T., Brown, D., Smith, L. B., Summers, K. M., Mabbott, N. A., Piccardo, P., Cheeseman, M. T., Burdon, T., Hume, D. A.
The American Association of Immunologists (AAI)
0022-1767
00221767
1550-6606
15506606
shingle_catch_all_3 Pleiotropic Impacts of Macrophage and Microglial Deficiency on Development in Rats with Targeted Mutation of the Csf1r Locus [IMMUNE SYSTEM DEVELOPMENT]
We have produced Csf1r -deficient rats by homologous recombination in embryonic stem cells. Consistent with the role of Csf1r in macrophage differentiation, there was a loss of peripheral blood monocytes, microglia in the brain, epidermal Langerhans cells, splenic marginal zone macrophages, bone-associated macrophages and osteoclasts, and peritoneal macrophages. Macrophages of splenic red pulp, liver, lung, and gut were less affected. The pleiotropic impacts of the loss of macrophages on development of multiple organ systems in rats were distinct from those reported in mice. Csf1r –/– rats survived well into adulthood with postnatal growth retardation, distinct skeletal and bone marrow abnormalities, infertility, and loss of visceral adipose tissue. Gene expression analysis in spleen revealed selective loss of transcripts associated with the marginal zone and, in brain regions, the loss of known and candidate novel microglia-associated transcripts. Despite the complete absence of microglia, there was little overt phenotype in brain, aside from reduced myelination and increased expression of dopamine receptor-associated transcripts in striatum. The results highlight the redundant and nonredundant functions of CSF1R signaling and of macrophages in development, organogenesis, and homeostasis.
Pridans, C., Raper, A., Davis, G. M., Alves, J., Sauter, K. A., Lefevre, L., Regan, T., Meek, S., Sutherland, L., Thomson, A. J., Clohisey, S., Bush, S. J., Rojo, R., Lisowski, Z. M., Wallace, R., Grabert, K., Upton, K. R., Tsai, Y. T., Brown, D., Smith, L. B., Summers, K. M., Mabbott, N. A., Piccardo, P., Cheeseman, M. T., Burdon, T., Hume, D. A.
The American Association of Immunologists (AAI)
0022-1767
00221767
1550-6606
15506606
shingle_catch_all_4 Pleiotropic Impacts of Macrophage and Microglial Deficiency on Development in Rats with Targeted Mutation of the Csf1r Locus [IMMUNE SYSTEM DEVELOPMENT]
We have produced Csf1r -deficient rats by homologous recombination in embryonic stem cells. Consistent with the role of Csf1r in macrophage differentiation, there was a loss of peripheral blood monocytes, microglia in the brain, epidermal Langerhans cells, splenic marginal zone macrophages, bone-associated macrophages and osteoclasts, and peritoneal macrophages. Macrophages of splenic red pulp, liver, lung, and gut were less affected. The pleiotropic impacts of the loss of macrophages on development of multiple organ systems in rats were distinct from those reported in mice. Csf1r –/– rats survived well into adulthood with postnatal growth retardation, distinct skeletal and bone marrow abnormalities, infertility, and loss of visceral adipose tissue. Gene expression analysis in spleen revealed selective loss of transcripts associated with the marginal zone and, in brain regions, the loss of known and candidate novel microglia-associated transcripts. Despite the complete absence of microglia, there was little overt phenotype in brain, aside from reduced myelination and increased expression of dopamine receptor-associated transcripts in striatum. The results highlight the redundant and nonredundant functions of CSF1R signaling and of macrophages in development, organogenesis, and homeostasis.
Pridans, C., Raper, A., Davis, G. M., Alves, J., Sauter, K. A., Lefevre, L., Regan, T., Meek, S., Sutherland, L., Thomson, A. J., Clohisey, S., Bush, S. J., Rojo, R., Lisowski, Z. M., Wallace, R., Grabert, K., Upton, K. R., Tsai, Y. T., Brown, D., Smith, L. B., Summers, K. M., Mabbott, N. A., Piccardo, P., Cheeseman, M. T., Burdon, T., Hume, D. A.
The American Association of Immunologists (AAI)
0022-1767
00221767
1550-6606
15506606
shingle_title_1 Pleiotropic Impacts of Macrophage and Microglial Deficiency on Development in Rats with Targeted Mutation of the Csf1r Locus [IMMUNE SYSTEM DEVELOPMENT]
shingle_title_2 Pleiotropic Impacts of Macrophage and Microglial Deficiency on Development in Rats with Targeted Mutation of the Csf1r Locus [IMMUNE SYSTEM DEVELOPMENT]
shingle_title_3 Pleiotropic Impacts of Macrophage and Microglial Deficiency on Development in Rats with Targeted Mutation of the Csf1r Locus [IMMUNE SYSTEM DEVELOPMENT]
shingle_title_4 Pleiotropic Impacts of Macrophage and Microglial Deficiency on Development in Rats with Targeted Mutation of the Csf1r Locus [IMMUNE SYSTEM DEVELOPMENT]
timestamp 2025-06-30T23:37:09.516Z
titel Pleiotropic Impacts of Macrophage and Microglial Deficiency on Development in Rats with Targeted Mutation of the Csf1r Locus [IMMUNE SYSTEM DEVELOPMENT]
titel_suche Pleiotropic Impacts of Macrophage and Microglial Deficiency on Development in Rats with Targeted Mutation of the Csf1r Locus [IMMUNE SYSTEM DEVELOPMENT]
topic WW-YZ
uid ipn_articles_6347309