Dissection of progenitor compartments resolves developmental trajectories in B-lymphopoiesis

Publication Date:
2018-07-03
Publisher:
Rockefeller University Press
Print ISSN:
0022-1007
Electronic ISSN:
1540-9538
Topics:
Medicine
Keywords:
Hematopoiesis
Published by:
http://www.rupress.org/
_version_ 1836398996521746432
autor Jensen, C. T., Ahsberg, J., Sommarin, M. N. E., Strid, T., Somasundaram, R., Okuyama, K., Ungerbäck, J., Kupari, J., Airaksinen, M. S., Lang, S., Bryder, D., Soneji, S., Karlsson, G., Sigvardsson, M.
beschreibung To understand the developmental trajectories in early lymphocyte differentiation, we identified differentially expressed surface markers on lineage-negative lymphoid progenitors (LPs). Single-cell polymerase chain reaction experiments allowed us to link surface marker expression to that of lineage-associated transcription factors (TFs) and identify GFRA2 and BST1 as markers of early B cells. Functional analyses in vitro and in vivo as well as single-cell gene expression analyses supported that surface expression of these proteins defined distinct subpopulations that include cells from both the classical common LPs (CLPs) and Fraction A compartments. The formation of the GFRA2-expressing stages of development depended on the TF EBF1, critical both for the activation of stage-specific target genes and modulation of the epigenetic landscape. Our data show that consecutive expression of Ly6D, GFRA2, and BST1 defines a developmental trajectory linking the CLP to the CD19 + progenitor compartment.
citation_standardnr 6297484
datenlieferant ipn_articles
feed_id 96
feed_publisher Rockefeller University Press
feed_publisher_url http://www.rupress.org/
insertion_date 2018-07-03
journaleissn 1540-9538
journalissn 0022-1007
publikationsjahr_anzeige 2018
publikationsjahr_facette 2018
publikationsjahr_intervall 7984:2015-2019
publikationsjahr_sort 2018
publisher Rockefeller University Press
quelle Journal of Experimental Medicine
relation http://jem.rupress.org/cgi/content/short/215/7/1947?rss=1
schlagwort Hematopoiesis
search_space articles
shingle_author_1 Jensen, C. T., Ahsberg, J., Sommarin, M. N. E., Strid, T., Somasundaram, R., Okuyama, K., Ungerbäck, J., Kupari, J., Airaksinen, M. S., Lang, S., Bryder, D., Soneji, S., Karlsson, G., Sigvardsson, M.
shingle_author_2 Jensen, C. T., Ahsberg, J., Sommarin, M. N. E., Strid, T., Somasundaram, R., Okuyama, K., Ungerbäck, J., Kupari, J., Airaksinen, M. S., Lang, S., Bryder, D., Soneji, S., Karlsson, G., Sigvardsson, M.
shingle_author_3 Jensen, C. T., Ahsberg, J., Sommarin, M. N. E., Strid, T., Somasundaram, R., Okuyama, K., Ungerbäck, J., Kupari, J., Airaksinen, M. S., Lang, S., Bryder, D., Soneji, S., Karlsson, G., Sigvardsson, M.
shingle_author_4 Jensen, C. T., Ahsberg, J., Sommarin, M. N. E., Strid, T., Somasundaram, R., Okuyama, K., Ungerbäck, J., Kupari, J., Airaksinen, M. S., Lang, S., Bryder, D., Soneji, S., Karlsson, G., Sigvardsson, M.
shingle_catch_all_1 Dissection of progenitor compartments resolves developmental trajectories in B-lymphopoiesis
Hematopoiesis
To understand the developmental trajectories in early lymphocyte differentiation, we identified differentially expressed surface markers on lineage-negative lymphoid progenitors (LPs). Single-cell polymerase chain reaction experiments allowed us to link surface marker expression to that of lineage-associated transcription factors (TFs) and identify GFRA2 and BST1 as markers of early B cells. Functional analyses in vitro and in vivo as well as single-cell gene expression analyses supported that surface expression of these proteins defined distinct subpopulations that include cells from both the classical common LPs (CLPs) and Fraction A compartments. The formation of the GFRA2-expressing stages of development depended on the TF EBF1, critical both for the activation of stage-specific target genes and modulation of the epigenetic landscape. Our data show that consecutive expression of Ly6D, GFRA2, and BST1 defines a developmental trajectory linking the CLP to the CD19 + progenitor compartment.
Jensen, C. T., Ahsberg, J., Sommarin, M. N. E., Strid, T., Somasundaram, R., Okuyama, K., Ungerbäck, J., Kupari, J., Airaksinen, M. S., Lang, S., Bryder, D., Soneji, S., Karlsson, G., Sigvardsson, M.
Rockefeller University Press
0022-1007
00221007
1540-9538
15409538
shingle_catch_all_2 Dissection of progenitor compartments resolves developmental trajectories in B-lymphopoiesis
Hematopoiesis
To understand the developmental trajectories in early lymphocyte differentiation, we identified differentially expressed surface markers on lineage-negative lymphoid progenitors (LPs). Single-cell polymerase chain reaction experiments allowed us to link surface marker expression to that of lineage-associated transcription factors (TFs) and identify GFRA2 and BST1 as markers of early B cells. Functional analyses in vitro and in vivo as well as single-cell gene expression analyses supported that surface expression of these proteins defined distinct subpopulations that include cells from both the classical common LPs (CLPs) and Fraction A compartments. The formation of the GFRA2-expressing stages of development depended on the TF EBF1, critical both for the activation of stage-specific target genes and modulation of the epigenetic landscape. Our data show that consecutive expression of Ly6D, GFRA2, and BST1 defines a developmental trajectory linking the CLP to the CD19 + progenitor compartment.
Jensen, C. T., Ahsberg, J., Sommarin, M. N. E., Strid, T., Somasundaram, R., Okuyama, K., Ungerbäck, J., Kupari, J., Airaksinen, M. S., Lang, S., Bryder, D., Soneji, S., Karlsson, G., Sigvardsson, M.
Rockefeller University Press
0022-1007
00221007
1540-9538
15409538
shingle_catch_all_3 Dissection of progenitor compartments resolves developmental trajectories in B-lymphopoiesis
Hematopoiesis
To understand the developmental trajectories in early lymphocyte differentiation, we identified differentially expressed surface markers on lineage-negative lymphoid progenitors (LPs). Single-cell polymerase chain reaction experiments allowed us to link surface marker expression to that of lineage-associated transcription factors (TFs) and identify GFRA2 and BST1 as markers of early B cells. Functional analyses in vitro and in vivo as well as single-cell gene expression analyses supported that surface expression of these proteins defined distinct subpopulations that include cells from both the classical common LPs (CLPs) and Fraction A compartments. The formation of the GFRA2-expressing stages of development depended on the TF EBF1, critical both for the activation of stage-specific target genes and modulation of the epigenetic landscape. Our data show that consecutive expression of Ly6D, GFRA2, and BST1 defines a developmental trajectory linking the CLP to the CD19 + progenitor compartment.
Jensen, C. T., Ahsberg, J., Sommarin, M. N. E., Strid, T., Somasundaram, R., Okuyama, K., Ungerbäck, J., Kupari, J., Airaksinen, M. S., Lang, S., Bryder, D., Soneji, S., Karlsson, G., Sigvardsson, M.
Rockefeller University Press
0022-1007
00221007
1540-9538
15409538
shingle_catch_all_4 Dissection of progenitor compartments resolves developmental trajectories in B-lymphopoiesis
Hematopoiesis
To understand the developmental trajectories in early lymphocyte differentiation, we identified differentially expressed surface markers on lineage-negative lymphoid progenitors (LPs). Single-cell polymerase chain reaction experiments allowed us to link surface marker expression to that of lineage-associated transcription factors (TFs) and identify GFRA2 and BST1 as markers of early B cells. Functional analyses in vitro and in vivo as well as single-cell gene expression analyses supported that surface expression of these proteins defined distinct subpopulations that include cells from both the classical common LPs (CLPs) and Fraction A compartments. The formation of the GFRA2-expressing stages of development depended on the TF EBF1, critical both for the activation of stage-specific target genes and modulation of the epigenetic landscape. Our data show that consecutive expression of Ly6D, GFRA2, and BST1 defines a developmental trajectory linking the CLP to the CD19 + progenitor compartment.
Jensen, C. T., Ahsberg, J., Sommarin, M. N. E., Strid, T., Somasundaram, R., Okuyama, K., Ungerbäck, J., Kupari, J., Airaksinen, M. S., Lang, S., Bryder, D., Soneji, S., Karlsson, G., Sigvardsson, M.
Rockefeller University Press
0022-1007
00221007
1540-9538
15409538
shingle_title_1 Dissection of progenitor compartments resolves developmental trajectories in B-lymphopoiesis
shingle_title_2 Dissection of progenitor compartments resolves developmental trajectories in B-lymphopoiesis
shingle_title_3 Dissection of progenitor compartments resolves developmental trajectories in B-lymphopoiesis
shingle_title_4 Dissection of progenitor compartments resolves developmental trajectories in B-lymphopoiesis
timestamp 2025-06-30T23:35:57.134Z
titel Dissection of progenitor compartments resolves developmental trajectories in B-lymphopoiesis
titel_suche Dissection of progenitor compartments resolves developmental trajectories in B-lymphopoiesis
topic WW-YZ
uid ipn_articles_6297484