Bacteria as living patchy colloids: Phenotypic heterogeneity in surface adhesion

Publication Date:
2018-04-28
Publisher:
American Association for the Advancement of Science (AAAS)
Electronic ISSN:
2375-2548
Topics:
Natural Sciences in General
Published by:
http://www.aaas.org/
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autor Vissers, T., Brown, A. T., Koumakis, N., Dawson, A., Hermes, M., Schwarz-Linek, J., Schofield, A. B., French, J. M., Koutsos, V., Arlt, J., Martinez, V. A., Poon, W. C. K.
beschreibung Understanding and controlling the surface adhesion of pathogenic bacteria is of urgent biomedical importance. However, many aspects of this process remain unclear (for example, microscopic details of the initial adhesion and possible variations between individual cells). Using a new high-throughput method, we identify and follow many single cells within a clonal population of Escherichia coli near a glass surface. We find strong phenotypic heterogeneities: A fraction of the cells remain in the free (planktonic) state, whereas others adhere with an adhesion strength that itself exhibits phenotypic heterogeneity. We explain our observations using a patchy colloid model; cells bind with localized, adhesive patches, and the strength of adhesion is determined by the number of patches: Nonadherers have no patches, weak adherers bind with a single patch only, and strong adherers bind via a single or multiple patches. We discuss possible implications of our results for controlling bacterial adhesion in biomedical and other applications.
citation_standardnr 6246937
datenlieferant ipn_articles
feed_id 228416
feed_publisher American Association for the Advancement of Science (AAAS)
feed_publisher_url http://www.aaas.org/
insertion_date 2018-04-28
journaleissn 2375-2548
publikationsjahr_anzeige 2018
publikationsjahr_facette 2018
publikationsjahr_intervall 7984:2015-2019
publikationsjahr_sort 2018
publisher American Association for the Advancement of Science (AAAS)
quelle Science Advances
relation http://advances.sciencemag.org/cgi/content/short/4/4/eaao1170?rss=1
search_space articles
shingle_author_1 Vissers, T., Brown, A. T., Koumakis, N., Dawson, A., Hermes, M., Schwarz-Linek, J., Schofield, A. B., French, J. M., Koutsos, V., Arlt, J., Martinez, V. A., Poon, W. C. K.
shingle_author_2 Vissers, T., Brown, A. T., Koumakis, N., Dawson, A., Hermes, M., Schwarz-Linek, J., Schofield, A. B., French, J. M., Koutsos, V., Arlt, J., Martinez, V. A., Poon, W. C. K.
shingle_author_3 Vissers, T., Brown, A. T., Koumakis, N., Dawson, A., Hermes, M., Schwarz-Linek, J., Schofield, A. B., French, J. M., Koutsos, V., Arlt, J., Martinez, V. A., Poon, W. C. K.
shingle_author_4 Vissers, T., Brown, A. T., Koumakis, N., Dawson, A., Hermes, M., Schwarz-Linek, J., Schofield, A. B., French, J. M., Koutsos, V., Arlt, J., Martinez, V. A., Poon, W. C. K.
shingle_catch_all_1 Bacteria as living patchy colloids: Phenotypic heterogeneity in surface adhesion
Understanding and controlling the surface adhesion of pathogenic bacteria is of urgent biomedical importance. However, many aspects of this process remain unclear (for example, microscopic details of the initial adhesion and possible variations between individual cells). Using a new high-throughput method, we identify and follow many single cells within a clonal population of Escherichia coli near a glass surface. We find strong phenotypic heterogeneities: A fraction of the cells remain in the free (planktonic) state, whereas others adhere with an adhesion strength that itself exhibits phenotypic heterogeneity. We explain our observations using a patchy colloid model; cells bind with localized, adhesive patches, and the strength of adhesion is determined by the number of patches: Nonadherers have no patches, weak adherers bind with a single patch only, and strong adherers bind via a single or multiple patches. We discuss possible implications of our results for controlling bacterial adhesion in biomedical and other applications.
Vissers, T., Brown, A. T., Koumakis, N., Dawson, A., Hermes, M., Schwarz-Linek, J., Schofield, A. B., French, J. M., Koutsos, V., Arlt, J., Martinez, V. A., Poon, W. C. K.
American Association for the Advancement of Science (AAAS)
2375-2548
23752548
shingle_catch_all_2 Bacteria as living patchy colloids: Phenotypic heterogeneity in surface adhesion
Understanding and controlling the surface adhesion of pathogenic bacteria is of urgent biomedical importance. However, many aspects of this process remain unclear (for example, microscopic details of the initial adhesion and possible variations between individual cells). Using a new high-throughput method, we identify and follow many single cells within a clonal population of Escherichia coli near a glass surface. We find strong phenotypic heterogeneities: A fraction of the cells remain in the free (planktonic) state, whereas others adhere with an adhesion strength that itself exhibits phenotypic heterogeneity. We explain our observations using a patchy colloid model; cells bind with localized, adhesive patches, and the strength of adhesion is determined by the number of patches: Nonadherers have no patches, weak adherers bind with a single patch only, and strong adherers bind via a single or multiple patches. We discuss possible implications of our results for controlling bacterial adhesion in biomedical and other applications.
Vissers, T., Brown, A. T., Koumakis, N., Dawson, A., Hermes, M., Schwarz-Linek, J., Schofield, A. B., French, J. M., Koutsos, V., Arlt, J., Martinez, V. A., Poon, W. C. K.
American Association for the Advancement of Science (AAAS)
2375-2548
23752548
shingle_catch_all_3 Bacteria as living patchy colloids: Phenotypic heterogeneity in surface adhesion
Understanding and controlling the surface adhesion of pathogenic bacteria is of urgent biomedical importance. However, many aspects of this process remain unclear (for example, microscopic details of the initial adhesion and possible variations between individual cells). Using a new high-throughput method, we identify and follow many single cells within a clonal population of Escherichia coli near a glass surface. We find strong phenotypic heterogeneities: A fraction of the cells remain in the free (planktonic) state, whereas others adhere with an adhesion strength that itself exhibits phenotypic heterogeneity. We explain our observations using a patchy colloid model; cells bind with localized, adhesive patches, and the strength of adhesion is determined by the number of patches: Nonadherers have no patches, weak adherers bind with a single patch only, and strong adherers bind via a single or multiple patches. We discuss possible implications of our results for controlling bacterial adhesion in biomedical and other applications.
Vissers, T., Brown, A. T., Koumakis, N., Dawson, A., Hermes, M., Schwarz-Linek, J., Schofield, A. B., French, J. M., Koutsos, V., Arlt, J., Martinez, V. A., Poon, W. C. K.
American Association for the Advancement of Science (AAAS)
2375-2548
23752548
shingle_catch_all_4 Bacteria as living patchy colloids: Phenotypic heterogeneity in surface adhesion
Understanding and controlling the surface adhesion of pathogenic bacteria is of urgent biomedical importance. However, many aspects of this process remain unclear (for example, microscopic details of the initial adhesion and possible variations between individual cells). Using a new high-throughput method, we identify and follow many single cells within a clonal population of Escherichia coli near a glass surface. We find strong phenotypic heterogeneities: A fraction of the cells remain in the free (planktonic) state, whereas others adhere with an adhesion strength that itself exhibits phenotypic heterogeneity. We explain our observations using a patchy colloid model; cells bind with localized, adhesive patches, and the strength of adhesion is determined by the number of patches: Nonadherers have no patches, weak adherers bind with a single patch only, and strong adherers bind via a single or multiple patches. We discuss possible implications of our results for controlling bacterial adhesion in biomedical and other applications.
Vissers, T., Brown, A. T., Koumakis, N., Dawson, A., Hermes, M., Schwarz-Linek, J., Schofield, A. B., French, J. M., Koutsos, V., Arlt, J., Martinez, V. A., Poon, W. C. K.
American Association for the Advancement of Science (AAAS)
2375-2548
23752548
shingle_title_1 Bacteria as living patchy colloids: Phenotypic heterogeneity in surface adhesion
shingle_title_2 Bacteria as living patchy colloids: Phenotypic heterogeneity in surface adhesion
shingle_title_3 Bacteria as living patchy colloids: Phenotypic heterogeneity in surface adhesion
shingle_title_4 Bacteria as living patchy colloids: Phenotypic heterogeneity in surface adhesion
timestamp 2025-06-30T23:34:36.819Z
titel Bacteria as living patchy colloids: Phenotypic heterogeneity in surface adhesion
titel_suche Bacteria as living patchy colloids: Phenotypic heterogeneity in surface adhesion
topic TA-TD
uid ipn_articles_6246937