Late neuroprogenitors contribute to normal retinal vascular development in a Hif2a-dependent manner [RESEARCH ARTICLE]

Publication Date:
2018-04-26
Publisher:
The Company of Biologists
Print ISSN:
0950-1991
Electronic ISSN:
1477-9129
Topics:
Biology
Keywords:
Cardiovascular development and regeneration, Neural development
Published by:
http://www.biologists.com/
_version_ 1839208028919824384
autor Cristante, E., Liyanage, S. E., Sampson, R. D., Kalargyrou, A., De Rossi, G., Rizzi, M., Hoke, J., Ribeiro, J., Maswood, R. N., Duran, Y., Matsuki, T., Aghaizu, N. D., Luhmann, U. F., Smith, A. J., Ali, R. R., Bainbridge, J. W. B.
beschreibung Enrico Cristante, Sidath E. Liyanage, Robert D. Sampson, Aikaterini Kalargyrou, Giulia De Rossi, Matteo Rizzi, Justin Hoke, Joana Ribeiro, Ryea N. Maswood, Yanai Duran, Takaaki Matsuki, Nozie D. Aghaizu, Ulrich F. Luhmann, Alexander J. Smith, Robin R. Ali, and James W. B. Bainbridge In the adult central nervous system, endothelial and neuronal cells engage in tight cross-talk as key components of the so-called neurovascular unit. Impairment of this important relationship adversely affects tissue homeostasis, as observed in neurodegenerative conditions including Alzheimer's and Parkinson's disease. In development, the influence of neuroprogenitor cells on angiogenesis is poorly understood. Here, we show in mouse that these cells interact intimately with the growing retinal vascular network, and we identify a novel regulatory mechanism of vasculature development mediated by hypoxia-inducible factor 2a (Hif2a). By Cre-lox gene excision, we show that Hif2a in retinal neuroprogenitor cells upregulates the expression of the pro-angiogenic mediators vascular endothelial growth factor and erythropoietin, whereas it locally downregulates the angiogenesis inhibitor endostatin. Importantly, absence of Hif2a in retinal neuroprogenitor cells causes a marked reduction of proliferating endothelial cells at the angiogenic front. This results in delayed retinal vascular development, fewer major retinal vessels and reduced density of the peripheral deep retinal vascular plexus. Our findings demonstrate that retinal neuroprogenitor cells are a crucial component of the developing neurovascular unit.
citation_standardnr 6244334
datenlieferant ipn_articles
feed_id 1748
feed_publisher The Company of Biologists
feed_publisher_url http://www.biologists.com/
insertion_date 2018-04-26
journaleissn 1477-9129
journalissn 0950-1991
publikationsjahr_anzeige 2018
publikationsjahr_facette 2018
publikationsjahr_intervall 7984:2015-2019
publikationsjahr_sort 2018
publisher The Company of Biologists
quelle Development
relation http://dev.biologists.org/cgi/content/short/145/8/dev157511?rss=1
schlagwort Cardiovascular development and regeneration, Neural development
search_space articles
shingle_author_1 Cristante, E., Liyanage, S. E., Sampson, R. D., Kalargyrou, A., De Rossi, G., Rizzi, M., Hoke, J., Ribeiro, J., Maswood, R. N., Duran, Y., Matsuki, T., Aghaizu, N. D., Luhmann, U. F., Smith, A. J., Ali, R. R., Bainbridge, J. W. B.
shingle_author_2 Cristante, E., Liyanage, S. E., Sampson, R. D., Kalargyrou, A., De Rossi, G., Rizzi, M., Hoke, J., Ribeiro, J., Maswood, R. N., Duran, Y., Matsuki, T., Aghaizu, N. D., Luhmann, U. F., Smith, A. J., Ali, R. R., Bainbridge, J. W. B.
shingle_author_3 Cristante, E., Liyanage, S. E., Sampson, R. D., Kalargyrou, A., De Rossi, G., Rizzi, M., Hoke, J., Ribeiro, J., Maswood, R. N., Duran, Y., Matsuki, T., Aghaizu, N. D., Luhmann, U. F., Smith, A. J., Ali, R. R., Bainbridge, J. W. B.
shingle_author_4 Cristante, E., Liyanage, S. E., Sampson, R. D., Kalargyrou, A., De Rossi, G., Rizzi, M., Hoke, J., Ribeiro, J., Maswood, R. N., Duran, Y., Matsuki, T., Aghaizu, N. D., Luhmann, U. F., Smith, A. J., Ali, R. R., Bainbridge, J. W. B.
shingle_catch_all_1 Late neuroprogenitors contribute to normal retinal vascular development in a Hif2a-dependent manner [RESEARCH ARTICLE]
Cardiovascular development and regeneration, Neural development
Enrico Cristante, Sidath E. Liyanage, Robert D. Sampson, Aikaterini Kalargyrou, Giulia De Rossi, Matteo Rizzi, Justin Hoke, Joana Ribeiro, Ryea N. Maswood, Yanai Duran, Takaaki Matsuki, Nozie D. Aghaizu, Ulrich F. Luhmann, Alexander J. Smith, Robin R. Ali, and James W. B. Bainbridge In the adult central nervous system, endothelial and neuronal cells engage in tight cross-talk as key components of the so-called neurovascular unit. Impairment of this important relationship adversely affects tissue homeostasis, as observed in neurodegenerative conditions including Alzheimer's and Parkinson's disease. In development, the influence of neuroprogenitor cells on angiogenesis is poorly understood. Here, we show in mouse that these cells interact intimately with the growing retinal vascular network, and we identify a novel regulatory mechanism of vasculature development mediated by hypoxia-inducible factor 2a (Hif2a). By Cre-lox gene excision, we show that Hif2a in retinal neuroprogenitor cells upregulates the expression of the pro-angiogenic mediators vascular endothelial growth factor and erythropoietin, whereas it locally downregulates the angiogenesis inhibitor endostatin. Importantly, absence of Hif2a in retinal neuroprogenitor cells causes a marked reduction of proliferating endothelial cells at the angiogenic front. This results in delayed retinal vascular development, fewer major retinal vessels and reduced density of the peripheral deep retinal vascular plexus. Our findings demonstrate that retinal neuroprogenitor cells are a crucial component of the developing neurovascular unit.
Cristante, E., Liyanage, S. E., Sampson, R. D., Kalargyrou, A., De Rossi, G., Rizzi, M., Hoke, J., Ribeiro, J., Maswood, R. N., Duran, Y., Matsuki, T., Aghaizu, N. D., Luhmann, U. F., Smith, A. J., Ali, R. R., Bainbridge, J. W. B.
The Company of Biologists
0950-1991
09501991
1477-9129
14779129
shingle_catch_all_2 Late neuroprogenitors contribute to normal retinal vascular development in a Hif2a-dependent manner [RESEARCH ARTICLE]
Cardiovascular development and regeneration, Neural development
Enrico Cristante, Sidath E. Liyanage, Robert D. Sampson, Aikaterini Kalargyrou, Giulia De Rossi, Matteo Rizzi, Justin Hoke, Joana Ribeiro, Ryea N. Maswood, Yanai Duran, Takaaki Matsuki, Nozie D. Aghaizu, Ulrich F. Luhmann, Alexander J. Smith, Robin R. Ali, and James W. B. Bainbridge In the adult central nervous system, endothelial and neuronal cells engage in tight cross-talk as key components of the so-called neurovascular unit. Impairment of this important relationship adversely affects tissue homeostasis, as observed in neurodegenerative conditions including Alzheimer's and Parkinson's disease. In development, the influence of neuroprogenitor cells on angiogenesis is poorly understood. Here, we show in mouse that these cells interact intimately with the growing retinal vascular network, and we identify a novel regulatory mechanism of vasculature development mediated by hypoxia-inducible factor 2a (Hif2a). By Cre-lox gene excision, we show that Hif2a in retinal neuroprogenitor cells upregulates the expression of the pro-angiogenic mediators vascular endothelial growth factor and erythropoietin, whereas it locally downregulates the angiogenesis inhibitor endostatin. Importantly, absence of Hif2a in retinal neuroprogenitor cells causes a marked reduction of proliferating endothelial cells at the angiogenic front. This results in delayed retinal vascular development, fewer major retinal vessels and reduced density of the peripheral deep retinal vascular plexus. Our findings demonstrate that retinal neuroprogenitor cells are a crucial component of the developing neurovascular unit.
Cristante, E., Liyanage, S. E., Sampson, R. D., Kalargyrou, A., De Rossi, G., Rizzi, M., Hoke, J., Ribeiro, J., Maswood, R. N., Duran, Y., Matsuki, T., Aghaizu, N. D., Luhmann, U. F., Smith, A. J., Ali, R. R., Bainbridge, J. W. B.
The Company of Biologists
0950-1991
09501991
1477-9129
14779129
shingle_catch_all_3 Late neuroprogenitors contribute to normal retinal vascular development in a Hif2a-dependent manner [RESEARCH ARTICLE]
Cardiovascular development and regeneration, Neural development
Enrico Cristante, Sidath E. Liyanage, Robert D. Sampson, Aikaterini Kalargyrou, Giulia De Rossi, Matteo Rizzi, Justin Hoke, Joana Ribeiro, Ryea N. Maswood, Yanai Duran, Takaaki Matsuki, Nozie D. Aghaizu, Ulrich F. Luhmann, Alexander J. Smith, Robin R. Ali, and James W. B. Bainbridge In the adult central nervous system, endothelial and neuronal cells engage in tight cross-talk as key components of the so-called neurovascular unit. Impairment of this important relationship adversely affects tissue homeostasis, as observed in neurodegenerative conditions including Alzheimer's and Parkinson's disease. In development, the influence of neuroprogenitor cells on angiogenesis is poorly understood. Here, we show in mouse that these cells interact intimately with the growing retinal vascular network, and we identify a novel regulatory mechanism of vasculature development mediated by hypoxia-inducible factor 2a (Hif2a). By Cre-lox gene excision, we show that Hif2a in retinal neuroprogenitor cells upregulates the expression of the pro-angiogenic mediators vascular endothelial growth factor and erythropoietin, whereas it locally downregulates the angiogenesis inhibitor endostatin. Importantly, absence of Hif2a in retinal neuroprogenitor cells causes a marked reduction of proliferating endothelial cells at the angiogenic front. This results in delayed retinal vascular development, fewer major retinal vessels and reduced density of the peripheral deep retinal vascular plexus. Our findings demonstrate that retinal neuroprogenitor cells are a crucial component of the developing neurovascular unit.
Cristante, E., Liyanage, S. E., Sampson, R. D., Kalargyrou, A., De Rossi, G., Rizzi, M., Hoke, J., Ribeiro, J., Maswood, R. N., Duran, Y., Matsuki, T., Aghaizu, N. D., Luhmann, U. F., Smith, A. J., Ali, R. R., Bainbridge, J. W. B.
The Company of Biologists
0950-1991
09501991
1477-9129
14779129
shingle_catch_all_4 Late neuroprogenitors contribute to normal retinal vascular development in a Hif2a-dependent manner [RESEARCH ARTICLE]
Cardiovascular development and regeneration, Neural development
Enrico Cristante, Sidath E. Liyanage, Robert D. Sampson, Aikaterini Kalargyrou, Giulia De Rossi, Matteo Rizzi, Justin Hoke, Joana Ribeiro, Ryea N. Maswood, Yanai Duran, Takaaki Matsuki, Nozie D. Aghaizu, Ulrich F. Luhmann, Alexander J. Smith, Robin R. Ali, and James W. B. Bainbridge In the adult central nervous system, endothelial and neuronal cells engage in tight cross-talk as key components of the so-called neurovascular unit. Impairment of this important relationship adversely affects tissue homeostasis, as observed in neurodegenerative conditions including Alzheimer's and Parkinson's disease. In development, the influence of neuroprogenitor cells on angiogenesis is poorly understood. Here, we show in mouse that these cells interact intimately with the growing retinal vascular network, and we identify a novel regulatory mechanism of vasculature development mediated by hypoxia-inducible factor 2a (Hif2a). By Cre-lox gene excision, we show that Hif2a in retinal neuroprogenitor cells upregulates the expression of the pro-angiogenic mediators vascular endothelial growth factor and erythropoietin, whereas it locally downregulates the angiogenesis inhibitor endostatin. Importantly, absence of Hif2a in retinal neuroprogenitor cells causes a marked reduction of proliferating endothelial cells at the angiogenic front. This results in delayed retinal vascular development, fewer major retinal vessels and reduced density of the peripheral deep retinal vascular plexus. Our findings demonstrate that retinal neuroprogenitor cells are a crucial component of the developing neurovascular unit.
Cristante, E., Liyanage, S. E., Sampson, R. D., Kalargyrou, A., De Rossi, G., Rizzi, M., Hoke, J., Ribeiro, J., Maswood, R. N., Duran, Y., Matsuki, T., Aghaizu, N. D., Luhmann, U. F., Smith, A. J., Ali, R. R., Bainbridge, J. W. B.
The Company of Biologists
0950-1991
09501991
1477-9129
14779129
shingle_title_1 Late neuroprogenitors contribute to normal retinal vascular development in a Hif2a-dependent manner [RESEARCH ARTICLE]
shingle_title_2 Late neuroprogenitors contribute to normal retinal vascular development in a Hif2a-dependent manner [RESEARCH ARTICLE]
shingle_title_3 Late neuroprogenitors contribute to normal retinal vascular development in a Hif2a-dependent manner [RESEARCH ARTICLE]
shingle_title_4 Late neuroprogenitors contribute to normal retinal vascular development in a Hif2a-dependent manner [RESEARCH ARTICLE]
timestamp 2025-07-31T23:44:18.695Z
titel Late neuroprogenitors contribute to normal retinal vascular development in a Hif2a-dependent manner [RESEARCH ARTICLE]
titel_suche Late neuroprogenitors contribute to normal retinal vascular development in a Hif2a-dependent manner [RESEARCH ARTICLE]
topic W
uid ipn_articles_6244334