Microcrystalline Tyrosine and Aluminum as Adjuvants in Allergen-Specific Immunotherapy Protect from IgE-Mediated Reactivity in Mouse Models and Act Independently of Inflammasome and TLR Signaling [IMMUNOTHERAPY AND VACCINES]

Leuthard, D. S., Duda, A., Freiberger, S. N., Weiss, S., Dommann, I., Fenini, G., Contassot, E., Kramer, M. F., Skinner, M. A., Kündig, T. M., Heath, M. D., Johansen, P.
The American Association of Immunologists (AAI)
Published 2018

Publication Date:	2018-04-24
Publisher:	The American Association of Immunologists (AAI)
Print ISSN:	0022-1767
Electronic ISSN:	1550-6606
Topics:	Medicine
Published by:	http://www.aai.org/

Staff View

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autor	Leuthard, D. S., Duda, A., Freiberger, S. N., Weiss, S., Dommann, I., Fenini, G., Contassot, E., Kramer, M. F., Skinner, M. A., Kündig, T. M., Heath, M. D., Johansen, P.
beschreibung	Allergen immunotherapy (AIT) is the only modality that can modify immune responses to allergen exposure, but therapeutic coverage is low. One strategy to improve AIT safety and efficacy is the use of new or improved adjuvants. This study investigates immune responses produced by microcrystalline tyrosine (MCT)–based vaccines as compared with conventional aluminum hydroxide (alum). Wild-type, immune-signaling–deficient, and TCR-transgenic mice were treated with different Ags (e.g., OVA and cat dander Fel d 1), plus MCT or alum as depot adjuvants. Specific Ab responses in serum were measured by ELISA, whereas cytokine secretion was measured both in culture supernatants by ELISA or by flow cytometry of spleen cells. Upon initiation of AIT in allergic mice, body temperature and further clinical signs were used as indicators for anaphylaxis. Overall, MCT and alum induced comparable B and T cell responses, which were independent of TLR signaling. Alum induced stronger IgE and IL-4 secretion than MCT. MCT and alum induced caspase-dependent IL-1β secretion in human monocytes in vitro, but inflammasome activation had no functional effect on inflammatory and Ab responses measured in vivo. In sensitized mice, AIT with MCT-adjuvanted allergens caused fewer anaphylactic reactions compared with alum-adjuvanted allergens. As depot adjuvants, MCT and alum are comparably effective in strength and mechanism of Ag-specific IgG induction and induction of T cell responses. The biocompatible and biodegradable MCT seems therefore a suitable alternative adjuvant to alum-based vaccines and AIT.
citation_standardnr	6242845
datenlieferant	ipn_articles
feed_id	333
feed_publisher	The American Association of Immunologists (AAI)
feed_publisher_url	http://www.aai.org/
insertion_date	2018-04-24
journaleissn	1550-6606
journalissn	0022-1767
publikationsjahr_anzeige	2018
publikationsjahr_facette	2018
publikationsjahr_intervall	7984:2015-2019
publikationsjahr_sort	2018
publisher	The American Association of Immunologists (AAI)
quelle	Journal of Immunology
relation	http://www.jimmunol.org/cgi/content/short/200/9/3151?rss=1
search_space	articles
shingle_author_1	Leuthard, D. S., Duda, A., Freiberger, S. N., Weiss, S., Dommann, I., Fenini, G., Contassot, E., Kramer, M. F., Skinner, M. A., Kündig, T. M., Heath, M. D., Johansen, P.
shingle_author_2	Leuthard, D. S., Duda, A., Freiberger, S. N., Weiss, S., Dommann, I., Fenini, G., Contassot, E., Kramer, M. F., Skinner, M. A., Kündig, T. M., Heath, M. D., Johansen, P.
shingle_author_3	Leuthard, D. S., Duda, A., Freiberger, S. N., Weiss, S., Dommann, I., Fenini, G., Contassot, E., Kramer, M. F., Skinner, M. A., Kündig, T. M., Heath, M. D., Johansen, P.
shingle_author_4	Leuthard, D. S., Duda, A., Freiberger, S. N., Weiss, S., Dommann, I., Fenini, G., Contassot, E., Kramer, M. F., Skinner, M. A., Kündig, T. M., Heath, M. D., Johansen, P.
shingle_catch_all_1	Microcrystalline Tyrosine and Aluminum as Adjuvants in Allergen-Specific Immunotherapy Protect from IgE-Mediated Reactivity in Mouse Models and Act Independently of Inflammasome and TLR Signaling [IMMUNOTHERAPY AND VACCINES] Allergen immunotherapy (AIT) is the only modality that can modify immune responses to allergen exposure, but therapeutic coverage is low. One strategy to improve AIT safety and efficacy is the use of new or improved adjuvants. This study investigates immune responses produced by microcrystalline tyrosine (MCT)–based vaccines as compared with conventional aluminum hydroxide (alum). Wild-type, immune-signaling–deficient, and TCR-transgenic mice were treated with different Ags (e.g., OVA and cat dander Fel d 1), plus MCT or alum as depot adjuvants. Specific Ab responses in serum were measured by ELISA, whereas cytokine secretion was measured both in culture supernatants by ELISA or by flow cytometry of spleen cells. Upon initiation of AIT in allergic mice, body temperature and further clinical signs were used as indicators for anaphylaxis. Overall, MCT and alum induced comparable B and T cell responses, which were independent of TLR signaling. Alum induced stronger IgE and IL-4 secretion than MCT. MCT and alum induced caspase-dependent IL-1β secretion in human monocytes in vitro, but inflammasome activation had no functional effect on inflammatory and Ab responses measured in vivo. In sensitized mice, AIT with MCT-adjuvanted allergens caused fewer anaphylactic reactions compared with alum-adjuvanted allergens. As depot adjuvants, MCT and alum are comparably effective in strength and mechanism of Ag-specific IgG induction and induction of T cell responses. The biocompatible and biodegradable MCT seems therefore a suitable alternative adjuvant to alum-based vaccines and AIT. Leuthard, D. S., Duda, A., Freiberger, S. N., Weiss, S., Dommann, I., Fenini, G., Contassot, E., Kramer, M. F., Skinner, M. A., Kündig, T. M., Heath, M. D., Johansen, P. The American Association of Immunologists (AAI) 0022-1767 00221767 1550-6606 15506606
shingle_catch_all_2	Microcrystalline Tyrosine and Aluminum as Adjuvants in Allergen-Specific Immunotherapy Protect from IgE-Mediated Reactivity in Mouse Models and Act Independently of Inflammasome and TLR Signaling [IMMUNOTHERAPY AND VACCINES] Allergen immunotherapy (AIT) is the only modality that can modify immune responses to allergen exposure, but therapeutic coverage is low. One strategy to improve AIT safety and efficacy is the use of new or improved adjuvants. This study investigates immune responses produced by microcrystalline tyrosine (MCT)–based vaccines as compared with conventional aluminum hydroxide (alum). Wild-type, immune-signaling–deficient, and TCR-transgenic mice were treated with different Ags (e.g., OVA and cat dander Fel d 1), plus MCT or alum as depot adjuvants. Specific Ab responses in serum were measured by ELISA, whereas cytokine secretion was measured both in culture supernatants by ELISA or by flow cytometry of spleen cells. Upon initiation of AIT in allergic mice, body temperature and further clinical signs were used as indicators for anaphylaxis. Overall, MCT and alum induced comparable B and T cell responses, which were independent of TLR signaling. Alum induced stronger IgE and IL-4 secretion than MCT. MCT and alum induced caspase-dependent IL-1β secretion in human monocytes in vitro, but inflammasome activation had no functional effect on inflammatory and Ab responses measured in vivo. In sensitized mice, AIT with MCT-adjuvanted allergens caused fewer anaphylactic reactions compared with alum-adjuvanted allergens. As depot adjuvants, MCT and alum are comparably effective in strength and mechanism of Ag-specific IgG induction and induction of T cell responses. The biocompatible and biodegradable MCT seems therefore a suitable alternative adjuvant to alum-based vaccines and AIT. Leuthard, D. S., Duda, A., Freiberger, S. N., Weiss, S., Dommann, I., Fenini, G., Contassot, E., Kramer, M. F., Skinner, M. A., Kündig, T. M., Heath, M. D., Johansen, P. The American Association of Immunologists (AAI) 0022-1767 00221767 1550-6606 15506606
shingle_catch_all_3	Microcrystalline Tyrosine and Aluminum as Adjuvants in Allergen-Specific Immunotherapy Protect from IgE-Mediated Reactivity in Mouse Models and Act Independently of Inflammasome and TLR Signaling [IMMUNOTHERAPY AND VACCINES] Allergen immunotherapy (AIT) is the only modality that can modify immune responses to allergen exposure, but therapeutic coverage is low. One strategy to improve AIT safety and efficacy is the use of new or improved adjuvants. This study investigates immune responses produced by microcrystalline tyrosine (MCT)–based vaccines as compared with conventional aluminum hydroxide (alum). Wild-type, immune-signaling–deficient, and TCR-transgenic mice were treated with different Ags (e.g., OVA and cat dander Fel d 1), plus MCT or alum as depot adjuvants. Specific Ab responses in serum were measured by ELISA, whereas cytokine secretion was measured both in culture supernatants by ELISA or by flow cytometry of spleen cells. Upon initiation of AIT in allergic mice, body temperature and further clinical signs were used as indicators for anaphylaxis. Overall, MCT and alum induced comparable B and T cell responses, which were independent of TLR signaling. Alum induced stronger IgE and IL-4 secretion than MCT. MCT and alum induced caspase-dependent IL-1β secretion in human monocytes in vitro, but inflammasome activation had no functional effect on inflammatory and Ab responses measured in vivo. In sensitized mice, AIT with MCT-adjuvanted allergens caused fewer anaphylactic reactions compared with alum-adjuvanted allergens. As depot adjuvants, MCT and alum are comparably effective in strength and mechanism of Ag-specific IgG induction and induction of T cell responses. The biocompatible and biodegradable MCT seems therefore a suitable alternative adjuvant to alum-based vaccines and AIT. Leuthard, D. S., Duda, A., Freiberger, S. N., Weiss, S., Dommann, I., Fenini, G., Contassot, E., Kramer, M. F., Skinner, M. A., Kündig, T. M., Heath, M. D., Johansen, P. The American Association of Immunologists (AAI) 0022-1767 00221767 1550-6606 15506606
shingle_catch_all_4	Microcrystalline Tyrosine and Aluminum as Adjuvants in Allergen-Specific Immunotherapy Protect from IgE-Mediated Reactivity in Mouse Models and Act Independently of Inflammasome and TLR Signaling [IMMUNOTHERAPY AND VACCINES] Allergen immunotherapy (AIT) is the only modality that can modify immune responses to allergen exposure, but therapeutic coverage is low. One strategy to improve AIT safety and efficacy is the use of new or improved adjuvants. This study investigates immune responses produced by microcrystalline tyrosine (MCT)–based vaccines as compared with conventional aluminum hydroxide (alum). Wild-type, immune-signaling–deficient, and TCR-transgenic mice were treated with different Ags (e.g., OVA and cat dander Fel d 1), plus MCT or alum as depot adjuvants. Specific Ab responses in serum were measured by ELISA, whereas cytokine secretion was measured both in culture supernatants by ELISA or by flow cytometry of spleen cells. Upon initiation of AIT in allergic mice, body temperature and further clinical signs were used as indicators for anaphylaxis. Overall, MCT and alum induced comparable B and T cell responses, which were independent of TLR signaling. Alum induced stronger IgE and IL-4 secretion than MCT. MCT and alum induced caspase-dependent IL-1β secretion in human monocytes in vitro, but inflammasome activation had no functional effect on inflammatory and Ab responses measured in vivo. In sensitized mice, AIT with MCT-adjuvanted allergens caused fewer anaphylactic reactions compared with alum-adjuvanted allergens. As depot adjuvants, MCT and alum are comparably effective in strength and mechanism of Ag-specific IgG induction and induction of T cell responses. The biocompatible and biodegradable MCT seems therefore a suitable alternative adjuvant to alum-based vaccines and AIT. Leuthard, D. S., Duda, A., Freiberger, S. N., Weiss, S., Dommann, I., Fenini, G., Contassot, E., Kramer, M. F., Skinner, M. A., Kündig, T. M., Heath, M. D., Johansen, P. The American Association of Immunologists (AAI) 0022-1767 00221767 1550-6606 15506606
shingle_title_1	Microcrystalline Tyrosine and Aluminum as Adjuvants in Allergen-Specific Immunotherapy Protect from IgE-Mediated Reactivity in Mouse Models and Act Independently of Inflammasome and TLR Signaling [IMMUNOTHERAPY AND VACCINES]
shingle_title_2	Microcrystalline Tyrosine and Aluminum as Adjuvants in Allergen-Specific Immunotherapy Protect from IgE-Mediated Reactivity in Mouse Models and Act Independently of Inflammasome and TLR Signaling [IMMUNOTHERAPY AND VACCINES]
shingle_title_3	Microcrystalline Tyrosine and Aluminum as Adjuvants in Allergen-Specific Immunotherapy Protect from IgE-Mediated Reactivity in Mouse Models and Act Independently of Inflammasome and TLR Signaling [IMMUNOTHERAPY AND VACCINES]
shingle_title_4	Microcrystalline Tyrosine and Aluminum as Adjuvants in Allergen-Specific Immunotherapy Protect from IgE-Mediated Reactivity in Mouse Models and Act Independently of Inflammasome and TLR Signaling [IMMUNOTHERAPY AND VACCINES]
timestamp	2025-06-30T23:34:30.739Z
titel	Microcrystalline Tyrosine and Aluminum as Adjuvants in Allergen-Specific Immunotherapy Protect from IgE-Mediated Reactivity in Mouse Models and Act Independently of Inflammasome and TLR Signaling [IMMUNOTHERAPY AND VACCINES]
titel_suche	Microcrystalline Tyrosine and Aluminum as Adjuvants in Allergen-Specific Immunotherapy Protect from IgE-Mediated Reactivity in Mouse Models and Act Independently of Inflammasome and TLR Signaling [IMMUNOTHERAPY AND VACCINES]
topic	WW-YZ
uid	ipn_articles_6242845