Bacterial Community Dynamics across the Gastrointestinal Tracts of Dairy Calves during Preweaning Development [Microbial Ecology]

Publication Date:
2018-04-17
Publisher:
The American Society for Microbiology (ASM)
Print ISSN:
0099-2240
Electronic ISSN:
1098-5336
Topics:
Biology
Published by:
http://www.asm.org/
_version_ 1836398900334821376
autor Dias, J., Marcondes, M. I., Motta de Souza, S., Cardoso da Mata e Silva, B., Fontes Noronha, M., Tassinari Resende, R., Machado, F. S., Cuquetto Mantovani, H., Dill-Mc; Farland, K. A., Suen, G.
beschreibung Microbial communities play critical roles in the gastrointestinal tracts (GIT) of preruminant calves by influencing performance and health. However, little is known about the establishment of microbial communities in the calf GIT or their dynamics during development. In this study, next-generation sequencing was used to assess changes in the bacterial communities of the rumen, jejunum, cecum, and colon in 26 crossbred calves at four developmental stages (7, 28, 49, and 63 days old). Alpha diversity differed among GIT regions with the lowest diversity and evenness in the jejunum, whereas no changes in alpha diversity were observed across developmental stage. Beta diversity analysis showed both region and age effects, with low numbers of operational taxonomic units (OTUs) shared between regions within a given age group or between ages in a given region. Taxonomic analysis revealed that several taxa coexisted in the rumen, jejunum, cecum, and colon but that their abundances differed considerably by GIT region and age. As calves aged, we observed lower abundances of taxa such as Bacteroides , Parabacteroides , and Paraprevotella with higher abundances of Bulleidia and Succiniclasticum in the rumen. The jejunum also displayed taxonomic changes with increases in Clostridiaceae and Turicibacter taxa in older calves. In the lower gut, taxa such as Lactobacillus , Blautia , and Faecalibacterium decreased and S24-7, Paraprevotella , and Prevotella increased as calves aged. These data support a model whereby early and successive colonization by bacteria occurs across the GIT of calves and provides insights into the temporal dynamics of the GIT microbiota of dairy calves during preweaning development. IMPORTANCE The gastrointestinal tracts (GIT) of ruminants, such as dairy cows, house complex microbial communities that contribute to their overall health and support their ability to produce milk. For example, the rumen microbiota converts feed into usable nutrients, while the jejunal microbiota provides access to protein. Thus, establishing a properly functioning GIT microbiota in dairy calves is critical to their productivity as adult cows. However, little is known about the establishment, maintenance, and dynamics of the calf GIT microbiota in early life. In this study, we evaluated the bacterial communities in the rumen, jejunum, cecum, and colon in dairy calves across preweaning development and show that they are highly variable early on in life before transitioning to a stable community. Understanding the dairy calf GIT microbiota has implications for ensuring proper health during early life and will aid in efforts to develop strategies for improving downstream production.
citation_standardnr 6238562
datenlieferant ipn_articles
feed_id 516
feed_publisher The American Society for Microbiology (ASM)
feed_publisher_url http://www.asm.org/
insertion_date 2018-04-17
journaleissn 1098-5336
journalissn 0099-2240
publikationsjahr_anzeige 2018
publikationsjahr_facette 2018
publikationsjahr_intervall 7984:2015-2019
publikationsjahr_sort 2018
publisher The American Society for Microbiology (ASM)
quelle Applied and Environmental Microbiology
relation http://aem.asm.org/cgi/content/short/84/9/e02675-17?rss=1
search_space articles
shingle_author_1 Dias, J., Marcondes, M. I., Motta de Souza, S., Cardoso da Mata e Silva, B., Fontes Noronha, M., Tassinari Resende, R., Machado, F. S., Cuquetto Mantovani, H., Dill-Mc; Farland, K. A., Suen, G.
shingle_author_2 Dias, J., Marcondes, M. I., Motta de Souza, S., Cardoso da Mata e Silva, B., Fontes Noronha, M., Tassinari Resende, R., Machado, F. S., Cuquetto Mantovani, H., Dill-Mc; Farland, K. A., Suen, G.
shingle_author_3 Dias, J., Marcondes, M. I., Motta de Souza, S., Cardoso da Mata e Silva, B., Fontes Noronha, M., Tassinari Resende, R., Machado, F. S., Cuquetto Mantovani, H., Dill-Mc; Farland, K. A., Suen, G.
shingle_author_4 Dias, J., Marcondes, M. I., Motta de Souza, S., Cardoso da Mata e Silva, B., Fontes Noronha, M., Tassinari Resende, R., Machado, F. S., Cuquetto Mantovani, H., Dill-Mc; Farland, K. A., Suen, G.
shingle_catch_all_1 Bacterial Community Dynamics across the Gastrointestinal Tracts of Dairy Calves during Preweaning Development [Microbial Ecology]
Microbial communities play critical roles in the gastrointestinal tracts (GIT) of preruminant calves by influencing performance and health. However, little is known about the establishment of microbial communities in the calf GIT or their dynamics during development. In this study, next-generation sequencing was used to assess changes in the bacterial communities of the rumen, jejunum, cecum, and colon in 26 crossbred calves at four developmental stages (7, 28, 49, and 63 days old). Alpha diversity differed among GIT regions with the lowest diversity and evenness in the jejunum, whereas no changes in alpha diversity were observed across developmental stage. Beta diversity analysis showed both region and age effects, with low numbers of operational taxonomic units (OTUs) shared between regions within a given age group or between ages in a given region. Taxonomic analysis revealed that several taxa coexisted in the rumen, jejunum, cecum, and colon but that their abundances differed considerably by GIT region and age. As calves aged, we observed lower abundances of taxa such as Bacteroides , Parabacteroides , and Paraprevotella with higher abundances of Bulleidia and Succiniclasticum in the rumen. The jejunum also displayed taxonomic changes with increases in Clostridiaceae and Turicibacter taxa in older calves. In the lower gut, taxa such as Lactobacillus , Blautia , and Faecalibacterium decreased and S24-7, Paraprevotella , and Prevotella increased as calves aged. These data support a model whereby early and successive colonization by bacteria occurs across the GIT of calves and provides insights into the temporal dynamics of the GIT microbiota of dairy calves during preweaning development. IMPORTANCE The gastrointestinal tracts (GIT) of ruminants, such as dairy cows, house complex microbial communities that contribute to their overall health and support their ability to produce milk. For example, the rumen microbiota converts feed into usable nutrients, while the jejunal microbiota provides access to protein. Thus, establishing a properly functioning GIT microbiota in dairy calves is critical to their productivity as adult cows. However, little is known about the establishment, maintenance, and dynamics of the calf GIT microbiota in early life. In this study, we evaluated the bacterial communities in the rumen, jejunum, cecum, and colon in dairy calves across preweaning development and show that they are highly variable early on in life before transitioning to a stable community. Understanding the dairy calf GIT microbiota has implications for ensuring proper health during early life and will aid in efforts to develop strategies for improving downstream production.
Dias, J., Marcondes, M. I., Motta de Souza, S., Cardoso da Mata e Silva, B., Fontes Noronha, M., Tassinari Resende, R., Machado, F. S., Cuquetto Mantovani, H., Dill-Mc; Farland, K. A., Suen, G.
The American Society for Microbiology (ASM)
0099-2240
00992240
1098-5336
10985336
shingle_catch_all_2 Bacterial Community Dynamics across the Gastrointestinal Tracts of Dairy Calves during Preweaning Development [Microbial Ecology]
Microbial communities play critical roles in the gastrointestinal tracts (GIT) of preruminant calves by influencing performance and health. However, little is known about the establishment of microbial communities in the calf GIT or their dynamics during development. In this study, next-generation sequencing was used to assess changes in the bacterial communities of the rumen, jejunum, cecum, and colon in 26 crossbred calves at four developmental stages (7, 28, 49, and 63 days old). Alpha diversity differed among GIT regions with the lowest diversity and evenness in the jejunum, whereas no changes in alpha diversity were observed across developmental stage. Beta diversity analysis showed both region and age effects, with low numbers of operational taxonomic units (OTUs) shared between regions within a given age group or between ages in a given region. Taxonomic analysis revealed that several taxa coexisted in the rumen, jejunum, cecum, and colon but that their abundances differed considerably by GIT region and age. As calves aged, we observed lower abundances of taxa such as Bacteroides , Parabacteroides , and Paraprevotella with higher abundances of Bulleidia and Succiniclasticum in the rumen. The jejunum also displayed taxonomic changes with increases in Clostridiaceae and Turicibacter taxa in older calves. In the lower gut, taxa such as Lactobacillus , Blautia , and Faecalibacterium decreased and S24-7, Paraprevotella , and Prevotella increased as calves aged. These data support a model whereby early and successive colonization by bacteria occurs across the GIT of calves and provides insights into the temporal dynamics of the GIT microbiota of dairy calves during preweaning development. IMPORTANCE The gastrointestinal tracts (GIT) of ruminants, such as dairy cows, house complex microbial communities that contribute to their overall health and support their ability to produce milk. For example, the rumen microbiota converts feed into usable nutrients, while the jejunal microbiota provides access to protein. Thus, establishing a properly functioning GIT microbiota in dairy calves is critical to their productivity as adult cows. However, little is known about the establishment, maintenance, and dynamics of the calf GIT microbiota in early life. In this study, we evaluated the bacterial communities in the rumen, jejunum, cecum, and colon in dairy calves across preweaning development and show that they are highly variable early on in life before transitioning to a stable community. Understanding the dairy calf GIT microbiota has implications for ensuring proper health during early life and will aid in efforts to develop strategies for improving downstream production.
Dias, J., Marcondes, M. I., Motta de Souza, S., Cardoso da Mata e Silva, B., Fontes Noronha, M., Tassinari Resende, R., Machado, F. S., Cuquetto Mantovani, H., Dill-Mc; Farland, K. A., Suen, G.
The American Society for Microbiology (ASM)
0099-2240
00992240
1098-5336
10985336
shingle_catch_all_3 Bacterial Community Dynamics across the Gastrointestinal Tracts of Dairy Calves during Preweaning Development [Microbial Ecology]
Microbial communities play critical roles in the gastrointestinal tracts (GIT) of preruminant calves by influencing performance and health. However, little is known about the establishment of microbial communities in the calf GIT or their dynamics during development. In this study, next-generation sequencing was used to assess changes in the bacterial communities of the rumen, jejunum, cecum, and colon in 26 crossbred calves at four developmental stages (7, 28, 49, and 63 days old). Alpha diversity differed among GIT regions with the lowest diversity and evenness in the jejunum, whereas no changes in alpha diversity were observed across developmental stage. Beta diversity analysis showed both region and age effects, with low numbers of operational taxonomic units (OTUs) shared between regions within a given age group or between ages in a given region. Taxonomic analysis revealed that several taxa coexisted in the rumen, jejunum, cecum, and colon but that their abundances differed considerably by GIT region and age. As calves aged, we observed lower abundances of taxa such as Bacteroides , Parabacteroides , and Paraprevotella with higher abundances of Bulleidia and Succiniclasticum in the rumen. The jejunum also displayed taxonomic changes with increases in Clostridiaceae and Turicibacter taxa in older calves. In the lower gut, taxa such as Lactobacillus , Blautia , and Faecalibacterium decreased and S24-7, Paraprevotella , and Prevotella increased as calves aged. These data support a model whereby early and successive colonization by bacteria occurs across the GIT of calves and provides insights into the temporal dynamics of the GIT microbiota of dairy calves during preweaning development. IMPORTANCE The gastrointestinal tracts (GIT) of ruminants, such as dairy cows, house complex microbial communities that contribute to their overall health and support their ability to produce milk. For example, the rumen microbiota converts feed into usable nutrients, while the jejunal microbiota provides access to protein. Thus, establishing a properly functioning GIT microbiota in dairy calves is critical to their productivity as adult cows. However, little is known about the establishment, maintenance, and dynamics of the calf GIT microbiota in early life. In this study, we evaluated the bacterial communities in the rumen, jejunum, cecum, and colon in dairy calves across preweaning development and show that they are highly variable early on in life before transitioning to a stable community. Understanding the dairy calf GIT microbiota has implications for ensuring proper health during early life and will aid in efforts to develop strategies for improving downstream production.
Dias, J., Marcondes, M. I., Motta de Souza, S., Cardoso da Mata e Silva, B., Fontes Noronha, M., Tassinari Resende, R., Machado, F. S., Cuquetto Mantovani, H., Dill-Mc; Farland, K. A., Suen, G.
The American Society for Microbiology (ASM)
0099-2240
00992240
1098-5336
10985336
shingle_catch_all_4 Bacterial Community Dynamics across the Gastrointestinal Tracts of Dairy Calves during Preweaning Development [Microbial Ecology]
Microbial communities play critical roles in the gastrointestinal tracts (GIT) of preruminant calves by influencing performance and health. However, little is known about the establishment of microbial communities in the calf GIT or their dynamics during development. In this study, next-generation sequencing was used to assess changes in the bacterial communities of the rumen, jejunum, cecum, and colon in 26 crossbred calves at four developmental stages (7, 28, 49, and 63 days old). Alpha diversity differed among GIT regions with the lowest diversity and evenness in the jejunum, whereas no changes in alpha diversity were observed across developmental stage. Beta diversity analysis showed both region and age effects, with low numbers of operational taxonomic units (OTUs) shared between regions within a given age group or between ages in a given region. Taxonomic analysis revealed that several taxa coexisted in the rumen, jejunum, cecum, and colon but that their abundances differed considerably by GIT region and age. As calves aged, we observed lower abundances of taxa such as Bacteroides , Parabacteroides , and Paraprevotella with higher abundances of Bulleidia and Succiniclasticum in the rumen. The jejunum also displayed taxonomic changes with increases in Clostridiaceae and Turicibacter taxa in older calves. In the lower gut, taxa such as Lactobacillus , Blautia , and Faecalibacterium decreased and S24-7, Paraprevotella , and Prevotella increased as calves aged. These data support a model whereby early and successive colonization by bacteria occurs across the GIT of calves and provides insights into the temporal dynamics of the GIT microbiota of dairy calves during preweaning development. IMPORTANCE The gastrointestinal tracts (GIT) of ruminants, such as dairy cows, house complex microbial communities that contribute to their overall health and support their ability to produce milk. For example, the rumen microbiota converts feed into usable nutrients, while the jejunal microbiota provides access to protein. Thus, establishing a properly functioning GIT microbiota in dairy calves is critical to their productivity as adult cows. However, little is known about the establishment, maintenance, and dynamics of the calf GIT microbiota in early life. In this study, we evaluated the bacterial communities in the rumen, jejunum, cecum, and colon in dairy calves across preweaning development and show that they are highly variable early on in life before transitioning to a stable community. Understanding the dairy calf GIT microbiota has implications for ensuring proper health during early life and will aid in efforts to develop strategies for improving downstream production.
Dias, J., Marcondes, M. I., Motta de Souza, S., Cardoso da Mata e Silva, B., Fontes Noronha, M., Tassinari Resende, R., Machado, F. S., Cuquetto Mantovani, H., Dill-Mc; Farland, K. A., Suen, G.
The American Society for Microbiology (ASM)
0099-2240
00992240
1098-5336
10985336
shingle_title_1 Bacterial Community Dynamics across the Gastrointestinal Tracts of Dairy Calves during Preweaning Development [Microbial Ecology]
shingle_title_2 Bacterial Community Dynamics across the Gastrointestinal Tracts of Dairy Calves during Preweaning Development [Microbial Ecology]
shingle_title_3 Bacterial Community Dynamics across the Gastrointestinal Tracts of Dairy Calves during Preweaning Development [Microbial Ecology]
shingle_title_4 Bacterial Community Dynamics across the Gastrointestinal Tracts of Dairy Calves during Preweaning Development [Microbial Ecology]
timestamp 2025-06-30T23:34:24.970Z
titel Bacterial Community Dynamics across the Gastrointestinal Tracts of Dairy Calves during Preweaning Development [Microbial Ecology]
titel_suche Bacterial Community Dynamics across the Gastrointestinal Tracts of Dairy Calves during Preweaning Development [Microbial Ecology]
topic W
uid ipn_articles_6238562