Forced lipophagy reveals that lipid droplets are required for early embryonic development in mouse [RESEARCH ARTICLE]
Tatsumi, T., Takayama, K., Ishii, S., Yamamoto, A., Hara, T., Minami, N., Miyasaka, N., Kubota, T., Matsuura, A., Itakura, E., Tsukamoto, S.
The Company of Biologists
Published 2018
The Company of Biologists
Published 2018
| Publication Date: |
2018-03-06
|
|---|---|
| Publisher: |
The Company of Biologists
|
| Print ISSN: |
0950-1991
|
| Electronic ISSN: |
1477-9129
|
| Topics: |
Biology
|
| Published by: |
| _version_ | 1836398818366586880 |
|---|---|
| autor | Tatsumi, T., Takayama, K., Ishii, S., Yamamoto, A., Hara, T., Minami, N., Miyasaka, N., Kubota, T., Matsuura, A., Itakura, E., Tsukamoto, S. |
| beschreibung | Takayuki Tatsumi, Kaori Takayama, Shunsuke Ishii, Atsushi Yamamoto, Taichi Hara, Naojiro Minami, Naoyuki Miyasaka, Toshiro Kubota, Akira Matsuura, Eisuke Itakura, and Satoshi Tsukamoto Although autophagy is classically viewed as a non-selective degradation system, recent studies have revealed that various forms of selective autophagy also play crucial physiological roles. However, the induction of selective autophagy is not well understood. In this study, we established a forced selective autophagy system using a fusion of an autophagy adaptor and a substrate-binding protein. In both mammalian cells and fertilized mouse embryos, efficient forced lipophagy was induced by expression of a fusion of p62 (Sqstm1) and a lipid droplet (LD)-binding domain. In mouse embryos, induction of forced lipophagy caused a reduction in LD size and number, and decreased the triglyceride level throughout embryonic development, resulting in developmental retardation. Furthermore, lipophagy-induced embryos could eliminate excess LDs and were tolerant of lipotoxicity. Thus, by inducing forced lipophagy, expression of the p62 fusion protein generated LD-depleted cells, revealing an unexpected role of LD during preimplantation development. |
| citation_standardnr | 6183466 |
| datenlieferant | ipn_articles |
| feed_id | 1748 |
| feed_publisher | The Company of Biologists |
| feed_publisher_url | http://www.biologists.com/ |
| insertion_date | 2018-03-06 |
| journaleissn | 1477-9129 |
| journalissn | 0950-1991 |
| publikationsjahr_anzeige | 2018 |
| publikationsjahr_facette | 2018 |
| publikationsjahr_intervall | 7984:2015-2019 |
| publikationsjahr_sort | 2018 |
| publisher | The Company of Biologists |
| quelle | Development |
| relation | http://dev.biologists.org/cgi/content/short/145/4/dev161893?rss=1 |
| search_space | articles |
| shingle_author_1 | Tatsumi, T., Takayama, K., Ishii, S., Yamamoto, A., Hara, T., Minami, N., Miyasaka, N., Kubota, T., Matsuura, A., Itakura, E., Tsukamoto, S. |
| shingle_author_2 | Tatsumi, T., Takayama, K., Ishii, S., Yamamoto, A., Hara, T., Minami, N., Miyasaka, N., Kubota, T., Matsuura, A., Itakura, E., Tsukamoto, S. |
| shingle_author_3 | Tatsumi, T., Takayama, K., Ishii, S., Yamamoto, A., Hara, T., Minami, N., Miyasaka, N., Kubota, T., Matsuura, A., Itakura, E., Tsukamoto, S. |
| shingle_author_4 | Tatsumi, T., Takayama, K., Ishii, S., Yamamoto, A., Hara, T., Minami, N., Miyasaka, N., Kubota, T., Matsuura, A., Itakura, E., Tsukamoto, S. |
| shingle_catch_all_1 | Forced lipophagy reveals that lipid droplets are required for early embryonic development in mouse [RESEARCH ARTICLE] Takayuki Tatsumi, Kaori Takayama, Shunsuke Ishii, Atsushi Yamamoto, Taichi Hara, Naojiro Minami, Naoyuki Miyasaka, Toshiro Kubota, Akira Matsuura, Eisuke Itakura, and Satoshi Tsukamoto Although autophagy is classically viewed as a non-selective degradation system, recent studies have revealed that various forms of selective autophagy also play crucial physiological roles. However, the induction of selective autophagy is not well understood. In this study, we established a forced selective autophagy system using a fusion of an autophagy adaptor and a substrate-binding protein. In both mammalian cells and fertilized mouse embryos, efficient forced lipophagy was induced by expression of a fusion of p62 (Sqstm1) and a lipid droplet (LD)-binding domain. In mouse embryos, induction of forced lipophagy caused a reduction in LD size and number, and decreased the triglyceride level throughout embryonic development, resulting in developmental retardation. Furthermore, lipophagy-induced embryos could eliminate excess LDs and were tolerant of lipotoxicity. Thus, by inducing forced lipophagy, expression of the p62 fusion protein generated LD-depleted cells, revealing an unexpected role of LD during preimplantation development. Tatsumi, T., Takayama, K., Ishii, S., Yamamoto, A., Hara, T., Minami, N., Miyasaka, N., Kubota, T., Matsuura, A., Itakura, E., Tsukamoto, S. The Company of Biologists 0950-1991 09501991 1477-9129 14779129 |
| shingle_catch_all_2 | Forced lipophagy reveals that lipid droplets are required for early embryonic development in mouse [RESEARCH ARTICLE] Takayuki Tatsumi, Kaori Takayama, Shunsuke Ishii, Atsushi Yamamoto, Taichi Hara, Naojiro Minami, Naoyuki Miyasaka, Toshiro Kubota, Akira Matsuura, Eisuke Itakura, and Satoshi Tsukamoto Although autophagy is classically viewed as a non-selective degradation system, recent studies have revealed that various forms of selective autophagy also play crucial physiological roles. However, the induction of selective autophagy is not well understood. In this study, we established a forced selective autophagy system using a fusion of an autophagy adaptor and a substrate-binding protein. In both mammalian cells and fertilized mouse embryos, efficient forced lipophagy was induced by expression of a fusion of p62 (Sqstm1) and a lipid droplet (LD)-binding domain. In mouse embryos, induction of forced lipophagy caused a reduction in LD size and number, and decreased the triglyceride level throughout embryonic development, resulting in developmental retardation. Furthermore, lipophagy-induced embryos could eliminate excess LDs and were tolerant of lipotoxicity. Thus, by inducing forced lipophagy, expression of the p62 fusion protein generated LD-depleted cells, revealing an unexpected role of LD during preimplantation development. Tatsumi, T., Takayama, K., Ishii, S., Yamamoto, A., Hara, T., Minami, N., Miyasaka, N., Kubota, T., Matsuura, A., Itakura, E., Tsukamoto, S. The Company of Biologists 0950-1991 09501991 1477-9129 14779129 |
| shingle_catch_all_3 | Forced lipophagy reveals that lipid droplets are required for early embryonic development in mouse [RESEARCH ARTICLE] Takayuki Tatsumi, Kaori Takayama, Shunsuke Ishii, Atsushi Yamamoto, Taichi Hara, Naojiro Minami, Naoyuki Miyasaka, Toshiro Kubota, Akira Matsuura, Eisuke Itakura, and Satoshi Tsukamoto Although autophagy is classically viewed as a non-selective degradation system, recent studies have revealed that various forms of selective autophagy also play crucial physiological roles. However, the induction of selective autophagy is not well understood. In this study, we established a forced selective autophagy system using a fusion of an autophagy adaptor and a substrate-binding protein. In both mammalian cells and fertilized mouse embryos, efficient forced lipophagy was induced by expression of a fusion of p62 (Sqstm1) and a lipid droplet (LD)-binding domain. In mouse embryos, induction of forced lipophagy caused a reduction in LD size and number, and decreased the triglyceride level throughout embryonic development, resulting in developmental retardation. Furthermore, lipophagy-induced embryos could eliminate excess LDs and were tolerant of lipotoxicity. Thus, by inducing forced lipophagy, expression of the p62 fusion protein generated LD-depleted cells, revealing an unexpected role of LD during preimplantation development. Tatsumi, T., Takayama, K., Ishii, S., Yamamoto, A., Hara, T., Minami, N., Miyasaka, N., Kubota, T., Matsuura, A., Itakura, E., Tsukamoto, S. The Company of Biologists 0950-1991 09501991 1477-9129 14779129 |
| shingle_catch_all_4 | Forced lipophagy reveals that lipid droplets are required for early embryonic development in mouse [RESEARCH ARTICLE] Takayuki Tatsumi, Kaori Takayama, Shunsuke Ishii, Atsushi Yamamoto, Taichi Hara, Naojiro Minami, Naoyuki Miyasaka, Toshiro Kubota, Akira Matsuura, Eisuke Itakura, and Satoshi Tsukamoto Although autophagy is classically viewed as a non-selective degradation system, recent studies have revealed that various forms of selective autophagy also play crucial physiological roles. However, the induction of selective autophagy is not well understood. In this study, we established a forced selective autophagy system using a fusion of an autophagy adaptor and a substrate-binding protein. In both mammalian cells and fertilized mouse embryos, efficient forced lipophagy was induced by expression of a fusion of p62 (Sqstm1) and a lipid droplet (LD)-binding domain. In mouse embryos, induction of forced lipophagy caused a reduction in LD size and number, and decreased the triglyceride level throughout embryonic development, resulting in developmental retardation. Furthermore, lipophagy-induced embryos could eliminate excess LDs and were tolerant of lipotoxicity. Thus, by inducing forced lipophagy, expression of the p62 fusion protein generated LD-depleted cells, revealing an unexpected role of LD during preimplantation development. Tatsumi, T., Takayama, K., Ishii, S., Yamamoto, A., Hara, T., Minami, N., Miyasaka, N., Kubota, T., Matsuura, A., Itakura, E., Tsukamoto, S. The Company of Biologists 0950-1991 09501991 1477-9129 14779129 |
| shingle_title_1 | Forced lipophagy reveals that lipid droplets are required for early embryonic development in mouse [RESEARCH ARTICLE] |
| shingle_title_2 | Forced lipophagy reveals that lipid droplets are required for early embryonic development in mouse [RESEARCH ARTICLE] |
| shingle_title_3 | Forced lipophagy reveals that lipid droplets are required for early embryonic development in mouse [RESEARCH ARTICLE] |
| shingle_title_4 | Forced lipophagy reveals that lipid droplets are required for early embryonic development in mouse [RESEARCH ARTICLE] |
| timestamp | 2025-06-30T23:33:07.637Z |
| titel | Forced lipophagy reveals that lipid droplets are required for early embryonic development in mouse [RESEARCH ARTICLE] |
| titel_suche | Forced lipophagy reveals that lipid droplets are required for early embryonic development in mouse [RESEARCH ARTICLE] |
| topic | W |
| uid | ipn_articles_6183466 |