Molecular Epidemiology of Dairy Cattle-Associated Escherichia coli Carrying blaCTX-M Genes in Washington State [Public and Environmental Health Microbiology]
Afema, J. A., Ahmed, S., Besser, T. E., Jones, L. P., Sischo, W. M., Davis, M. A.
The American Society for Microbiology (ASM)
Published 2018
The American Society for Microbiology (ASM)
Published 2018
| Publication Date: |
2018-03-06
|
|---|---|
| Publisher: |
The American Society for Microbiology (ASM)
|
| Print ISSN: |
0099-2240
|
| Electronic ISSN: |
1098-5336
|
| Topics: |
Biology
|
| Published by: |
| _version_ | 1836398816353320961 |
|---|---|
| autor | Afema, J. A., Ahmed, S., Besser, T. E., Jones, L. P., Sischo, W. M., Davis, M. A. |
| beschreibung | An increase in the prevalence of commensal Escherichia coli carrying bla CTX-M genes among dairy cattle was observed between 2008 and 2012 in Washington State. To study the molecular epidemiology of this change, we selected 126 bla CTX-M -positive and 126 bla CTX-M -negative isolates for determinations of the multilocus sequence types (MLSTs) and antibiotic resistance phenotypes from E. coli obtained during a previous study. For 99 isolates, we also determined the bla CTX-M alleles using PCR and sequencing and identified the replicon types of bla CTX-M -carrying plasmids. The bla CTX-M -negative E. coli isolates comprised 76 sequence types (STs) compared with 32 STs in bla CTX-M -positive E. coli isolates. The bla CTX-M -positive E. coli isolates formed three MLST clonal complexes, accounting for 83% of these isolates; 52% of bla CTX-M -negative E. coli isolates clustered into 10 clonal complexes, and the remainder were singletons. Overall, bla CTX-M -negative E. coli isolates had more diverse genotypes that were distinct to farms, whereas bla CTX-M -positive E. coli isolates had a clonal population structure and were widely disseminated on farms in both regions included in the study. Plasmid replicon types included IncI1 which predominated, followed by IncFIB and IncFIA/FIB. bla CTX-M-15 was the predominant CTX-M gene allele, followed by bla CTX-M-27 and bla CTX-M-14 . There was no significant association between plasmid replicon types and bacterial STs, and neither clonal complexes nor major plasmid groups were associated with two discrete dairy-farming regions of Washington State. IMPORTANCE Infections caused by extended-spectrum β-lactamase (ESBL)-producing Escherichia coli occur globally and present treatment challenges because of their resistance to multiple antimicrobial drugs. Cattle are potential reservoirs of ESBL-producing Enterobacteriaceae , and so understanding the causes of successful dissemination of bla CTX-M genes in commensal bacteria will inform future approaches for the prevention of antibiotic-resistant pathogen emergence. |
| citation_standardnr | 6179161 |
| datenlieferant | ipn_articles |
| feed_id | 516 |
| feed_publisher | The American Society for Microbiology (ASM) |
| feed_publisher_url | http://www.asm.org/ |
| insertion_date | 2018-03-06 |
| journaleissn | 1098-5336 |
| journalissn | 0099-2240 |
| publikationsjahr_anzeige | 2018 |
| publikationsjahr_facette | 2018 |
| publikationsjahr_intervall | 7984:2015-2019 |
| publikationsjahr_sort | 2018 |
| publisher | The American Society for Microbiology (ASM) |
| quelle | Applied and Environmental Microbiology |
| relation | http://aem.asm.org/cgi/content/short/84/6/e02430-17?rss=1 |
| search_space | articles |
| shingle_author_1 | Afema, J. A., Ahmed, S., Besser, T. E., Jones, L. P., Sischo, W. M., Davis, M. A. |
| shingle_author_2 | Afema, J. A., Ahmed, S., Besser, T. E., Jones, L. P., Sischo, W. M., Davis, M. A. |
| shingle_author_3 | Afema, J. A., Ahmed, S., Besser, T. E., Jones, L. P., Sischo, W. M., Davis, M. A. |
| shingle_author_4 | Afema, J. A., Ahmed, S., Besser, T. E., Jones, L. P., Sischo, W. M., Davis, M. A. |
| shingle_catch_all_1 | Molecular Epidemiology of Dairy Cattle-Associated Escherichia coli Carrying blaCTX-M Genes in Washington State [Public and Environmental Health Microbiology] An increase in the prevalence of commensal Escherichia coli carrying bla CTX-M genes among dairy cattle was observed between 2008 and 2012 in Washington State. To study the molecular epidemiology of this change, we selected 126 bla CTX-M -positive and 126 bla CTX-M -negative isolates for determinations of the multilocus sequence types (MLSTs) and antibiotic resistance phenotypes from E. coli obtained during a previous study. For 99 isolates, we also determined the bla CTX-M alleles using PCR and sequencing and identified the replicon types of bla CTX-M -carrying plasmids. The bla CTX-M -negative E. coli isolates comprised 76 sequence types (STs) compared with 32 STs in bla CTX-M -positive E. coli isolates. The bla CTX-M -positive E. coli isolates formed three MLST clonal complexes, accounting for 83% of these isolates; 52% of bla CTX-M -negative E. coli isolates clustered into 10 clonal complexes, and the remainder were singletons. Overall, bla CTX-M -negative E. coli isolates had more diverse genotypes that were distinct to farms, whereas bla CTX-M -positive E. coli isolates had a clonal population structure and were widely disseminated on farms in both regions included in the study. Plasmid replicon types included IncI1 which predominated, followed by IncFIB and IncFIA/FIB. bla CTX-M-15 was the predominant CTX-M gene allele, followed by bla CTX-M-27 and bla CTX-M-14 . There was no significant association between plasmid replicon types and bacterial STs, and neither clonal complexes nor major plasmid groups were associated with two discrete dairy-farming regions of Washington State. IMPORTANCE Infections caused by extended-spectrum β-lactamase (ESBL)-producing Escherichia coli occur globally and present treatment challenges because of their resistance to multiple antimicrobial drugs. Cattle are potential reservoirs of ESBL-producing Enterobacteriaceae , and so understanding the causes of successful dissemination of bla CTX-M genes in commensal bacteria will inform future approaches for the prevention of antibiotic-resistant pathogen emergence. Afema, J. A., Ahmed, S., Besser, T. E., Jones, L. P., Sischo, W. M., Davis, M. A. The American Society for Microbiology (ASM) 0099-2240 00992240 1098-5336 10985336 |
| shingle_catch_all_2 | Molecular Epidemiology of Dairy Cattle-Associated Escherichia coli Carrying blaCTX-M Genes in Washington State [Public and Environmental Health Microbiology] An increase in the prevalence of commensal Escherichia coli carrying bla CTX-M genes among dairy cattle was observed between 2008 and 2012 in Washington State. To study the molecular epidemiology of this change, we selected 126 bla CTX-M -positive and 126 bla CTX-M -negative isolates for determinations of the multilocus sequence types (MLSTs) and antibiotic resistance phenotypes from E. coli obtained during a previous study. For 99 isolates, we also determined the bla CTX-M alleles using PCR and sequencing and identified the replicon types of bla CTX-M -carrying plasmids. The bla CTX-M -negative E. coli isolates comprised 76 sequence types (STs) compared with 32 STs in bla CTX-M -positive E. coli isolates. The bla CTX-M -positive E. coli isolates formed three MLST clonal complexes, accounting for 83% of these isolates; 52% of bla CTX-M -negative E. coli isolates clustered into 10 clonal complexes, and the remainder were singletons. Overall, bla CTX-M -negative E. coli isolates had more diverse genotypes that were distinct to farms, whereas bla CTX-M -positive E. coli isolates had a clonal population structure and were widely disseminated on farms in both regions included in the study. Plasmid replicon types included IncI1 which predominated, followed by IncFIB and IncFIA/FIB. bla CTX-M-15 was the predominant CTX-M gene allele, followed by bla CTX-M-27 and bla CTX-M-14 . There was no significant association between plasmid replicon types and bacterial STs, and neither clonal complexes nor major plasmid groups were associated with two discrete dairy-farming regions of Washington State. IMPORTANCE Infections caused by extended-spectrum β-lactamase (ESBL)-producing Escherichia coli occur globally and present treatment challenges because of their resistance to multiple antimicrobial drugs. Cattle are potential reservoirs of ESBL-producing Enterobacteriaceae , and so understanding the causes of successful dissemination of bla CTX-M genes in commensal bacteria will inform future approaches for the prevention of antibiotic-resistant pathogen emergence. Afema, J. A., Ahmed, S., Besser, T. E., Jones, L. P., Sischo, W. M., Davis, M. A. The American Society for Microbiology (ASM) 0099-2240 00992240 1098-5336 10985336 |
| shingle_catch_all_3 | Molecular Epidemiology of Dairy Cattle-Associated Escherichia coli Carrying blaCTX-M Genes in Washington State [Public and Environmental Health Microbiology] An increase in the prevalence of commensal Escherichia coli carrying bla CTX-M genes among dairy cattle was observed between 2008 and 2012 in Washington State. To study the molecular epidemiology of this change, we selected 126 bla CTX-M -positive and 126 bla CTX-M -negative isolates for determinations of the multilocus sequence types (MLSTs) and antibiotic resistance phenotypes from E. coli obtained during a previous study. For 99 isolates, we also determined the bla CTX-M alleles using PCR and sequencing and identified the replicon types of bla CTX-M -carrying plasmids. The bla CTX-M -negative E. coli isolates comprised 76 sequence types (STs) compared with 32 STs in bla CTX-M -positive E. coli isolates. The bla CTX-M -positive E. coli isolates formed three MLST clonal complexes, accounting for 83% of these isolates; 52% of bla CTX-M -negative E. coli isolates clustered into 10 clonal complexes, and the remainder were singletons. Overall, bla CTX-M -negative E. coli isolates had more diverse genotypes that were distinct to farms, whereas bla CTX-M -positive E. coli isolates had a clonal population structure and were widely disseminated on farms in both regions included in the study. Plasmid replicon types included IncI1 which predominated, followed by IncFIB and IncFIA/FIB. bla CTX-M-15 was the predominant CTX-M gene allele, followed by bla CTX-M-27 and bla CTX-M-14 . There was no significant association between plasmid replicon types and bacterial STs, and neither clonal complexes nor major plasmid groups were associated with two discrete dairy-farming regions of Washington State. IMPORTANCE Infections caused by extended-spectrum β-lactamase (ESBL)-producing Escherichia coli occur globally and present treatment challenges because of their resistance to multiple antimicrobial drugs. Cattle are potential reservoirs of ESBL-producing Enterobacteriaceae , and so understanding the causes of successful dissemination of bla CTX-M genes in commensal bacteria will inform future approaches for the prevention of antibiotic-resistant pathogen emergence. Afema, J. A., Ahmed, S., Besser, T. E., Jones, L. P., Sischo, W. M., Davis, M. A. The American Society for Microbiology (ASM) 0099-2240 00992240 1098-5336 10985336 |
| shingle_catch_all_4 | Molecular Epidemiology of Dairy Cattle-Associated Escherichia coli Carrying blaCTX-M Genes in Washington State [Public and Environmental Health Microbiology] An increase in the prevalence of commensal Escherichia coli carrying bla CTX-M genes among dairy cattle was observed between 2008 and 2012 in Washington State. To study the molecular epidemiology of this change, we selected 126 bla CTX-M -positive and 126 bla CTX-M -negative isolates for determinations of the multilocus sequence types (MLSTs) and antibiotic resistance phenotypes from E. coli obtained during a previous study. For 99 isolates, we also determined the bla CTX-M alleles using PCR and sequencing and identified the replicon types of bla CTX-M -carrying plasmids. The bla CTX-M -negative E. coli isolates comprised 76 sequence types (STs) compared with 32 STs in bla CTX-M -positive E. coli isolates. The bla CTX-M -positive E. coli isolates formed three MLST clonal complexes, accounting for 83% of these isolates; 52% of bla CTX-M -negative E. coli isolates clustered into 10 clonal complexes, and the remainder were singletons. Overall, bla CTX-M -negative E. coli isolates had more diverse genotypes that were distinct to farms, whereas bla CTX-M -positive E. coli isolates had a clonal population structure and were widely disseminated on farms in both regions included in the study. Plasmid replicon types included IncI1 which predominated, followed by IncFIB and IncFIA/FIB. bla CTX-M-15 was the predominant CTX-M gene allele, followed by bla CTX-M-27 and bla CTX-M-14 . There was no significant association between plasmid replicon types and bacterial STs, and neither clonal complexes nor major plasmid groups were associated with two discrete dairy-farming regions of Washington State. IMPORTANCE Infections caused by extended-spectrum β-lactamase (ESBL)-producing Escherichia coli occur globally and present treatment challenges because of their resistance to multiple antimicrobial drugs. Cattle are potential reservoirs of ESBL-producing Enterobacteriaceae , and so understanding the causes of successful dissemination of bla CTX-M genes in commensal bacteria will inform future approaches for the prevention of antibiotic-resistant pathogen emergence. Afema, J. A., Ahmed, S., Besser, T. E., Jones, L. P., Sischo, W. M., Davis, M. A. The American Society for Microbiology (ASM) 0099-2240 00992240 1098-5336 10985336 |
| shingle_title_1 | Molecular Epidemiology of Dairy Cattle-Associated Escherichia coli Carrying blaCTX-M Genes in Washington State [Public and Environmental Health Microbiology] |
| shingle_title_2 | Molecular Epidemiology of Dairy Cattle-Associated Escherichia coli Carrying blaCTX-M Genes in Washington State [Public and Environmental Health Microbiology] |
| shingle_title_3 | Molecular Epidemiology of Dairy Cattle-Associated Escherichia coli Carrying blaCTX-M Genes in Washington State [Public and Environmental Health Microbiology] |
| shingle_title_4 | Molecular Epidemiology of Dairy Cattle-Associated Escherichia coli Carrying blaCTX-M Genes in Washington State [Public and Environmental Health Microbiology] |
| timestamp | 2025-06-30T23:33:05.200Z |
| titel | Molecular Epidemiology of Dairy Cattle-Associated Escherichia coli Carrying blaCTX-M Genes in Washington State [Public and Environmental Health Microbiology] |
| titel_suche | Molecular Epidemiology of Dairy Cattle-Associated Escherichia coli Carrying blaCTX-M Genes in Washington State [Public and Environmental Health Microbiology] |
| topic | W |
| uid | ipn_articles_6179161 |