Alternative Th17 and CD4+CD25+FoxP3+ cells frequency increase and correlate with worse cardiac function in Chagas cardiomyopathy

Publication Date:
2018-02-24
Publisher:
Wiley-Blackwell
Print ISSN:
0300-9475
Electronic ISSN:
1365-3083
Topics:
Medicine
Published by:
http://www.wiley.com/wiley-blackwell
_version_ 1836398811805646848
autor Mariana S. Almeida, Virginia M.B. Lorena, Carolina de Araújo Medeiros, Wilson Oliveira Junior, Maria da Glória A. M. Cavalcanti, Silvia M. Martins, Clarice N.L. de Morais
beschreibung Immune homeostasis has been suggested to play an important role in the clinical evolution of chronic Chagas disease, however, the immunopathologic factors involved haven't been fully elucidated. Therefore, our study aimed to analyze the frequency of CD4 + CD25 + FoxP3 + cells, classic Th17 cells, alternative Th17 cells and IL-17 + B cells from peripheral blood of chronic cardiac patients after in vitro stimulation with Trypanosoma cruzi soluble EPI antigen. Patients were selected and classified according to clinical evaluation of cardiac involvement: mild, B1 (CARD1) (n = 20), and severe, C (CARD2) (n = 11). Patients with the indeterminate form of CD were included as the control group A (IND) (n = 17). Blood samples were collected and cultured in the presence of EPI antigen. Cells frequency and median fluorescence intensity (MFI) were obtained by flow cytometry. Our results showed that only CD4 + CD25 + FoxP3 + , CD4 + CD25 high FoxP3 + , CD4 + IL-17 + IFN-γ - and CD4 + IL-17 + IFN-γ + cells are more frequent in patients with severe cardiac disease, and correlate with worse global cardiac function. However, while indeterminate patients demonstrated a positive correlation between CD4 + CD25 + FoxP3 + and CD4 + IL-17 + IFN-γ - Th17 cells, this relationship was not observed in cardiac patients. IL-17 expression by Th17 cells and B cells correlated with disease progression. Altogether our results suggest that the clinical progression of Chagas cardiomyopathy involves worsening of inflammation and impairment of immunoregulatory mechanisms. This article is protected by copyright. All rights reserved.
citation_standardnr 6174747
datenlieferant ipn_articles
feed_id 5433
feed_publisher Wiley-Blackwell
feed_publisher_url http://www.wiley.com/wiley-blackwell
insertion_date 2018-02-24
journaleissn 1365-3083
journalissn 0300-9475
publikationsjahr_anzeige 2018
publikationsjahr_facette 2018
publikationsjahr_intervall 7984:2015-2019
publikationsjahr_sort 2018
publisher Wiley-Blackwell
quelle Scandinavian Journal of Immunology
relation http://onlinelibrary.wiley.com/resolve/doi?DOI=10.1111%2Fsji.12650
search_space articles
shingle_author_1 Mariana S. Almeida, Virginia M.B. Lorena, Carolina de Araújo Medeiros, Wilson Oliveira Junior, Maria da Glória A. M. Cavalcanti, Silvia M. Martins, Clarice N.L. de Morais
shingle_author_2 Mariana S. Almeida, Virginia M.B. Lorena, Carolina de Araújo Medeiros, Wilson Oliveira Junior, Maria da Glória A. M. Cavalcanti, Silvia M. Martins, Clarice N.L. de Morais
shingle_author_3 Mariana S. Almeida, Virginia M.B. Lorena, Carolina de Araújo Medeiros, Wilson Oliveira Junior, Maria da Glória A. M. Cavalcanti, Silvia M. Martins, Clarice N.L. de Morais
shingle_author_4 Mariana S. Almeida, Virginia M.B. Lorena, Carolina de Araújo Medeiros, Wilson Oliveira Junior, Maria da Glória A. M. Cavalcanti, Silvia M. Martins, Clarice N.L. de Morais
shingle_catch_all_1 Alternative Th17 and CD4+CD25+FoxP3+ cells frequency increase and correlate with worse cardiac function in Chagas cardiomyopathy
Immune homeostasis has been suggested to play an important role in the clinical evolution of chronic Chagas disease, however, the immunopathologic factors involved haven't been fully elucidated. Therefore, our study aimed to analyze the frequency of CD4 + CD25 + FoxP3 + cells, classic Th17 cells, alternative Th17 cells and IL-17 + B cells from peripheral blood of chronic cardiac patients after in vitro stimulation with Trypanosoma cruzi soluble EPI antigen. Patients were selected and classified according to clinical evaluation of cardiac involvement: mild, B1 (CARD1) (n = 20), and severe, C (CARD2) (n = 11). Patients with the indeterminate form of CD were included as the control group A (IND) (n = 17). Blood samples were collected and cultured in the presence of EPI antigen. Cells frequency and median fluorescence intensity (MFI) were obtained by flow cytometry. Our results showed that only CD4 + CD25 + FoxP3 + , CD4 + CD25 high FoxP3 + , CD4 + IL-17 + IFN-γ - and CD4 + IL-17 + IFN-γ + cells are more frequent in patients with severe cardiac disease, and correlate with worse global cardiac function. However, while indeterminate patients demonstrated a positive correlation between CD4 + CD25 + FoxP3 + and CD4 + IL-17 + IFN-γ - Th17 cells, this relationship was not observed in cardiac patients. IL-17 expression by Th17 cells and B cells correlated with disease progression. Altogether our results suggest that the clinical progression of Chagas cardiomyopathy involves worsening of inflammation and impairment of immunoregulatory mechanisms. This article is protected by copyright. All rights reserved.
Mariana S. Almeida, Virginia M.B. Lorena, Carolina de Araújo Medeiros, Wilson Oliveira Junior, Maria da Glória A. M. Cavalcanti, Silvia M. Martins, Clarice N.L. de Morais
Wiley-Blackwell
0300-9475
03009475
1365-3083
13653083
shingle_catch_all_2 Alternative Th17 and CD4+CD25+FoxP3+ cells frequency increase and correlate with worse cardiac function in Chagas cardiomyopathy
Immune homeostasis has been suggested to play an important role in the clinical evolution of chronic Chagas disease, however, the immunopathologic factors involved haven't been fully elucidated. Therefore, our study aimed to analyze the frequency of CD4 + CD25 + FoxP3 + cells, classic Th17 cells, alternative Th17 cells and IL-17 + B cells from peripheral blood of chronic cardiac patients after in vitro stimulation with Trypanosoma cruzi soluble EPI antigen. Patients were selected and classified according to clinical evaluation of cardiac involvement: mild, B1 (CARD1) (n = 20), and severe, C (CARD2) (n = 11). Patients with the indeterminate form of CD were included as the control group A (IND) (n = 17). Blood samples were collected and cultured in the presence of EPI antigen. Cells frequency and median fluorescence intensity (MFI) were obtained by flow cytometry. Our results showed that only CD4 + CD25 + FoxP3 + , CD4 + CD25 high FoxP3 + , CD4 + IL-17 + IFN-γ - and CD4 + IL-17 + IFN-γ + cells are more frequent in patients with severe cardiac disease, and correlate with worse global cardiac function. However, while indeterminate patients demonstrated a positive correlation between CD4 + CD25 + FoxP3 + and CD4 + IL-17 + IFN-γ - Th17 cells, this relationship was not observed in cardiac patients. IL-17 expression by Th17 cells and B cells correlated with disease progression. Altogether our results suggest that the clinical progression of Chagas cardiomyopathy involves worsening of inflammation and impairment of immunoregulatory mechanisms. This article is protected by copyright. All rights reserved.
Mariana S. Almeida, Virginia M.B. Lorena, Carolina de Araújo Medeiros, Wilson Oliveira Junior, Maria da Glória A. M. Cavalcanti, Silvia M. Martins, Clarice N.L. de Morais
Wiley-Blackwell
0300-9475
03009475
1365-3083
13653083
shingle_catch_all_3 Alternative Th17 and CD4+CD25+FoxP3+ cells frequency increase and correlate with worse cardiac function in Chagas cardiomyopathy
Immune homeostasis has been suggested to play an important role in the clinical evolution of chronic Chagas disease, however, the immunopathologic factors involved haven't been fully elucidated. Therefore, our study aimed to analyze the frequency of CD4 + CD25 + FoxP3 + cells, classic Th17 cells, alternative Th17 cells and IL-17 + B cells from peripheral blood of chronic cardiac patients after in vitro stimulation with Trypanosoma cruzi soluble EPI antigen. Patients were selected and classified according to clinical evaluation of cardiac involvement: mild, B1 (CARD1) (n = 20), and severe, C (CARD2) (n = 11). Patients with the indeterminate form of CD were included as the control group A (IND) (n = 17). Blood samples were collected and cultured in the presence of EPI antigen. Cells frequency and median fluorescence intensity (MFI) were obtained by flow cytometry. Our results showed that only CD4 + CD25 + FoxP3 + , CD4 + CD25 high FoxP3 + , CD4 + IL-17 + IFN-γ - and CD4 + IL-17 + IFN-γ + cells are more frequent in patients with severe cardiac disease, and correlate with worse global cardiac function. However, while indeterminate patients demonstrated a positive correlation between CD4 + CD25 + FoxP3 + and CD4 + IL-17 + IFN-γ - Th17 cells, this relationship was not observed in cardiac patients. IL-17 expression by Th17 cells and B cells correlated with disease progression. Altogether our results suggest that the clinical progression of Chagas cardiomyopathy involves worsening of inflammation and impairment of immunoregulatory mechanisms. This article is protected by copyright. All rights reserved.
Mariana S. Almeida, Virginia M.B. Lorena, Carolina de Araújo Medeiros, Wilson Oliveira Junior, Maria da Glória A. M. Cavalcanti, Silvia M. Martins, Clarice N.L. de Morais
Wiley-Blackwell
0300-9475
03009475
1365-3083
13653083
shingle_catch_all_4 Alternative Th17 and CD4+CD25+FoxP3+ cells frequency increase and correlate with worse cardiac function in Chagas cardiomyopathy
Immune homeostasis has been suggested to play an important role in the clinical evolution of chronic Chagas disease, however, the immunopathologic factors involved haven't been fully elucidated. Therefore, our study aimed to analyze the frequency of CD4 + CD25 + FoxP3 + cells, classic Th17 cells, alternative Th17 cells and IL-17 + B cells from peripheral blood of chronic cardiac patients after in vitro stimulation with Trypanosoma cruzi soluble EPI antigen. Patients were selected and classified according to clinical evaluation of cardiac involvement: mild, B1 (CARD1) (n = 20), and severe, C (CARD2) (n = 11). Patients with the indeterminate form of CD were included as the control group A (IND) (n = 17). Blood samples were collected and cultured in the presence of EPI antigen. Cells frequency and median fluorescence intensity (MFI) were obtained by flow cytometry. Our results showed that only CD4 + CD25 + FoxP3 + , CD4 + CD25 high FoxP3 + , CD4 + IL-17 + IFN-γ - and CD4 + IL-17 + IFN-γ + cells are more frequent in patients with severe cardiac disease, and correlate with worse global cardiac function. However, while indeterminate patients demonstrated a positive correlation between CD4 + CD25 + FoxP3 + and CD4 + IL-17 + IFN-γ - Th17 cells, this relationship was not observed in cardiac patients. IL-17 expression by Th17 cells and B cells correlated with disease progression. Altogether our results suggest that the clinical progression of Chagas cardiomyopathy involves worsening of inflammation and impairment of immunoregulatory mechanisms. This article is protected by copyright. All rights reserved.
Mariana S. Almeida, Virginia M.B. Lorena, Carolina de Araújo Medeiros, Wilson Oliveira Junior, Maria da Glória A. M. Cavalcanti, Silvia M. Martins, Clarice N.L. de Morais
Wiley-Blackwell
0300-9475
03009475
1365-3083
13653083
shingle_title_1 Alternative Th17 and CD4+CD25+FoxP3+ cells frequency increase and correlate with worse cardiac function in Chagas cardiomyopathy
shingle_title_2 Alternative Th17 and CD4+CD25+FoxP3+ cells frequency increase and correlate with worse cardiac function in Chagas cardiomyopathy
shingle_title_3 Alternative Th17 and CD4+CD25+FoxP3+ cells frequency increase and correlate with worse cardiac function in Chagas cardiomyopathy
shingle_title_4 Alternative Th17 and CD4+CD25+FoxP3+ cells frequency increase and correlate with worse cardiac function in Chagas cardiomyopathy
timestamp 2025-06-30T23:33:01.096Z
titel Alternative Th17 and CD4+CD25+FoxP3+ cells frequency increase and correlate with worse cardiac function in Chagas cardiomyopathy
titel_suche Alternative Th17 and CD4+CD25+FoxP3+ cells frequency increase and correlate with worse cardiac function in Chagas cardiomyopathy
topic WW-YZ
uid ipn_articles_6174747