ES cell-derived presomitic mesoderm-like tissues for analysis of synchronized oscillations in the segmentation clock [STEM CELLS AND REGENERATION]

Publication Date:
2018-02-15
Publisher:
The Company of Biologists
Print ISSN:
0950-1991
Electronic ISSN:
1477-9129
Topics:
Biology
Keywords:
Stem cells & regeneration
Published by:
http://www.biologists.com/
_version_ 1836398793015164928
autor Matsumiya, M., Tomita, T., Yoshioka-Kobayashi, K., Isomura, A., Kageyama, R.
beschreibung Marina Matsumiya, Takehito Tomita, Kumiko Yoshioka-Kobayashi, Akihiro Isomura, and Ryoichiro Kageyama Somites are periodically formed by segmentation of the anterior parts of the presomitic mesoderm (PSM). In the mouse embryo, this periodicity is controlled by the segmentation clock gene Hes7 , which exhibits wave-like oscillatory expression in the PSM. Despite intensive studies, the exact mechanism of such synchronous oscillatory dynamics of Hes7 expression still remains to be analyzed. Detailed analysis of the segmentation clock has been hampered because it requires the use of live embryos, and establishment of an in vitro culture system would facilitate such analyses. Here, we established a simple and efficient method to generate mouse ES cell-derived PSM-like tissues, in which Hes7 expression oscillates like traveling waves. In these tissues, Hes7 oscillation is synchronized between neighboring cells, and the posterior-anterior axis is self-organized as the central-peripheral axis. This method is applicable to chemical-library screening and will facilitate the analysis of the molecular nature of the segmentation clock.
citation_standardnr 6165182
datenlieferant ipn_articles
feed_id 1748
feed_publisher The Company of Biologists
feed_publisher_url http://www.biologists.com/
insertion_date 2018-02-15
journaleissn 1477-9129
journalissn 0950-1991
publikationsjahr_anzeige 2018
publikationsjahr_facette 2018
publikationsjahr_intervall 7984:2015-2019
publikationsjahr_sort 2018
publisher The Company of Biologists
quelle Development
relation http://dev.biologists.org/cgi/content/short/145/4/dev156836?rss=1
schlagwort Stem cells & regeneration
search_space articles
shingle_author_1 Matsumiya, M., Tomita, T., Yoshioka-Kobayashi, K., Isomura, A., Kageyama, R.
shingle_author_2 Matsumiya, M., Tomita, T., Yoshioka-Kobayashi, K., Isomura, A., Kageyama, R.
shingle_author_3 Matsumiya, M., Tomita, T., Yoshioka-Kobayashi, K., Isomura, A., Kageyama, R.
shingle_author_4 Matsumiya, M., Tomita, T., Yoshioka-Kobayashi, K., Isomura, A., Kageyama, R.
shingle_catch_all_1 ES cell-derived presomitic mesoderm-like tissues for analysis of synchronized oscillations in the segmentation clock [STEM CELLS AND REGENERATION]
Stem cells & regeneration
Marina Matsumiya, Takehito Tomita, Kumiko Yoshioka-Kobayashi, Akihiro Isomura, and Ryoichiro Kageyama Somites are periodically formed by segmentation of the anterior parts of the presomitic mesoderm (PSM). In the mouse embryo, this periodicity is controlled by the segmentation clock gene Hes7 , which exhibits wave-like oscillatory expression in the PSM. Despite intensive studies, the exact mechanism of such synchronous oscillatory dynamics of Hes7 expression still remains to be analyzed. Detailed analysis of the segmentation clock has been hampered because it requires the use of live embryos, and establishment of an in vitro culture system would facilitate such analyses. Here, we established a simple and efficient method to generate mouse ES cell-derived PSM-like tissues, in which Hes7 expression oscillates like traveling waves. In these tissues, Hes7 oscillation is synchronized between neighboring cells, and the posterior-anterior axis is self-organized as the central-peripheral axis. This method is applicable to chemical-library screening and will facilitate the analysis of the molecular nature of the segmentation clock.
Matsumiya, M., Tomita, T., Yoshioka-Kobayashi, K., Isomura, A., Kageyama, R.
The Company of Biologists
0950-1991
09501991
1477-9129
14779129
shingle_catch_all_2 ES cell-derived presomitic mesoderm-like tissues for analysis of synchronized oscillations in the segmentation clock [STEM CELLS AND REGENERATION]
Stem cells & regeneration
Marina Matsumiya, Takehito Tomita, Kumiko Yoshioka-Kobayashi, Akihiro Isomura, and Ryoichiro Kageyama Somites are periodically formed by segmentation of the anterior parts of the presomitic mesoderm (PSM). In the mouse embryo, this periodicity is controlled by the segmentation clock gene Hes7 , which exhibits wave-like oscillatory expression in the PSM. Despite intensive studies, the exact mechanism of such synchronous oscillatory dynamics of Hes7 expression still remains to be analyzed. Detailed analysis of the segmentation clock has been hampered because it requires the use of live embryos, and establishment of an in vitro culture system would facilitate such analyses. Here, we established a simple and efficient method to generate mouse ES cell-derived PSM-like tissues, in which Hes7 expression oscillates like traveling waves. In these tissues, Hes7 oscillation is synchronized between neighboring cells, and the posterior-anterior axis is self-organized as the central-peripheral axis. This method is applicable to chemical-library screening and will facilitate the analysis of the molecular nature of the segmentation clock.
Matsumiya, M., Tomita, T., Yoshioka-Kobayashi, K., Isomura, A., Kageyama, R.
The Company of Biologists
0950-1991
09501991
1477-9129
14779129
shingle_catch_all_3 ES cell-derived presomitic mesoderm-like tissues for analysis of synchronized oscillations in the segmentation clock [STEM CELLS AND REGENERATION]
Stem cells & regeneration
Marina Matsumiya, Takehito Tomita, Kumiko Yoshioka-Kobayashi, Akihiro Isomura, and Ryoichiro Kageyama Somites are periodically formed by segmentation of the anterior parts of the presomitic mesoderm (PSM). In the mouse embryo, this periodicity is controlled by the segmentation clock gene Hes7 , which exhibits wave-like oscillatory expression in the PSM. Despite intensive studies, the exact mechanism of such synchronous oscillatory dynamics of Hes7 expression still remains to be analyzed. Detailed analysis of the segmentation clock has been hampered because it requires the use of live embryos, and establishment of an in vitro culture system would facilitate such analyses. Here, we established a simple and efficient method to generate mouse ES cell-derived PSM-like tissues, in which Hes7 expression oscillates like traveling waves. In these tissues, Hes7 oscillation is synchronized between neighboring cells, and the posterior-anterior axis is self-organized as the central-peripheral axis. This method is applicable to chemical-library screening and will facilitate the analysis of the molecular nature of the segmentation clock.
Matsumiya, M., Tomita, T., Yoshioka-Kobayashi, K., Isomura, A., Kageyama, R.
The Company of Biologists
0950-1991
09501991
1477-9129
14779129
shingle_catch_all_4 ES cell-derived presomitic mesoderm-like tissues for analysis of synchronized oscillations in the segmentation clock [STEM CELLS AND REGENERATION]
Stem cells & regeneration
Marina Matsumiya, Takehito Tomita, Kumiko Yoshioka-Kobayashi, Akihiro Isomura, and Ryoichiro Kageyama Somites are periodically formed by segmentation of the anterior parts of the presomitic mesoderm (PSM). In the mouse embryo, this periodicity is controlled by the segmentation clock gene Hes7 , which exhibits wave-like oscillatory expression in the PSM. Despite intensive studies, the exact mechanism of such synchronous oscillatory dynamics of Hes7 expression still remains to be analyzed. Detailed analysis of the segmentation clock has been hampered because it requires the use of live embryos, and establishment of an in vitro culture system would facilitate such analyses. Here, we established a simple and efficient method to generate mouse ES cell-derived PSM-like tissues, in which Hes7 expression oscillates like traveling waves. In these tissues, Hes7 oscillation is synchronized between neighboring cells, and the posterior-anterior axis is self-organized as the central-peripheral axis. This method is applicable to chemical-library screening and will facilitate the analysis of the molecular nature of the segmentation clock.
Matsumiya, M., Tomita, T., Yoshioka-Kobayashi, K., Isomura, A., Kageyama, R.
The Company of Biologists
0950-1991
09501991
1477-9129
14779129
shingle_title_1 ES cell-derived presomitic mesoderm-like tissues for analysis of synchronized oscillations in the segmentation clock [STEM CELLS AND REGENERATION]
shingle_title_2 ES cell-derived presomitic mesoderm-like tissues for analysis of synchronized oscillations in the segmentation clock [STEM CELLS AND REGENERATION]
shingle_title_3 ES cell-derived presomitic mesoderm-like tissues for analysis of synchronized oscillations in the segmentation clock [STEM CELLS AND REGENERATION]
shingle_title_4 ES cell-derived presomitic mesoderm-like tissues for analysis of synchronized oscillations in the segmentation clock [STEM CELLS AND REGENERATION]
timestamp 2025-06-30T23:32:43.159Z
titel ES cell-derived presomitic mesoderm-like tissues for analysis of synchronized oscillations in the segmentation clock [STEM CELLS AND REGENERATION]
titel_suche ES cell-derived presomitic mesoderm-like tissues for analysis of synchronized oscillations in the segmentation clock [STEM CELLS AND REGENERATION]
topic W
uid ipn_articles_6165182