A control for the day-to-day normalization of the flow cytometry γ-H2AX assay for clinical routine
| Publication Date: |
2018-02-08
|
|---|---|
| Publisher: |
Wiley-Blackwell
|
| Topics: |
Biology
Medicine
|
| Published by: |
| _version_ | 1836398782582882304 |
|---|---|
| autor | Adam Viktorisson, Sherin T Mathew, Ola Hammarsten, Pegah Johansson |
| beschreibung | Background: The phosphorylation of histone H2AX (γ-H2AX) at the DNA double-strand break (DSB) site is frequently used for quantifying DSBs and may be useful as a biomarker for clinical applications. We have previously reported a flow cytometry-based quantification of γ-H2AX for clinical routine. One major challenge, however, is the lack of a control sample for normalization of the day-to-day variation of the flow cytometry γ-H2AX assay. Methods: Here, we report development of a mix-control sample containing peripheral blood mononuclear cells (PBMC) from 10 control individuals, for normalization of day-to-day variation of the flow cytometry-γ-H2AX assay. Results: We showed that control individuals sampled on different days show an average day-to-day variation (CV) of 34%, which was reduced to 12% after normalization to the control sample. The normalization allowed detection of radiosensitivity of lymphoblastoid cell lines from ataxia telangiectasia patients, sampled over three days. Conclusion: The mix-control sample, consisting of 10 control individuals' PBMC, can be used as a control sample to normalize for day-to-day variation of the γ-H2AX assay. The use of this sample will facilitate integration of the γ-H2AX assay into clinical routine. This article is protected by copyright. All rights reserved. |
| citation_standardnr | 6158332 |
| datenlieferant | ipn_articles |
| feed_id | 40322 |
| feed_publisher | Wiley-Blackwell |
| feed_publisher_url | http://www.wiley.com/wiley-blackwell |
| insertion_date | 2018-02-08 |
| publikationsjahr_anzeige | 2018 |
| publikationsjahr_facette | 2018 |
| publikationsjahr_intervall | 7984:2015-2019 |
| publikationsjahr_sort | 2018 |
| publisher | Wiley-Blackwell |
| quelle | Cytometry. Part B |
| relation | http://onlinelibrary.wiley.com/resolve/doi?DOI=10.1002%2Fcyto.b.21627 |
| search_space | articles |
| shingle_author_1 | Adam Viktorisson, Sherin T Mathew, Ola Hammarsten, Pegah Johansson |
| shingle_author_2 | Adam Viktorisson, Sherin T Mathew, Ola Hammarsten, Pegah Johansson |
| shingle_author_3 | Adam Viktorisson, Sherin T Mathew, Ola Hammarsten, Pegah Johansson |
| shingle_author_4 | Adam Viktorisson, Sherin T Mathew, Ola Hammarsten, Pegah Johansson |
| shingle_catch_all_1 | A control for the day-to-day normalization of the flow cytometry γ-H2AX assay for clinical routine Background: The phosphorylation of histone H2AX (γ-H2AX) at the DNA double-strand break (DSB) site is frequently used for quantifying DSBs and may be useful as a biomarker for clinical applications. We have previously reported a flow cytometry-based quantification of γ-H2AX for clinical routine. One major challenge, however, is the lack of a control sample for normalization of the day-to-day variation of the flow cytometry γ-H2AX assay. Methods: Here, we report development of a mix-control sample containing peripheral blood mononuclear cells (PBMC) from 10 control individuals, for normalization of day-to-day variation of the flow cytometry-γ-H2AX assay. Results: We showed that control individuals sampled on different days show an average day-to-day variation (CV) of 34%, which was reduced to 12% after normalization to the control sample. The normalization allowed detection of radiosensitivity of lymphoblastoid cell lines from ataxia telangiectasia patients, sampled over three days. Conclusion: The mix-control sample, consisting of 10 control individuals' PBMC, can be used as a control sample to normalize for day-to-day variation of the γ-H2AX assay. The use of this sample will facilitate integration of the γ-H2AX assay into clinical routine. This article is protected by copyright. All rights reserved. Adam Viktorisson, Sherin T Mathew, Ola Hammarsten, Pegah Johansson Wiley-Blackwell |
| shingle_catch_all_2 | A control for the day-to-day normalization of the flow cytometry γ-H2AX assay for clinical routine Background: The phosphorylation of histone H2AX (γ-H2AX) at the DNA double-strand break (DSB) site is frequently used for quantifying DSBs and may be useful as a biomarker for clinical applications. We have previously reported a flow cytometry-based quantification of γ-H2AX for clinical routine. One major challenge, however, is the lack of a control sample for normalization of the day-to-day variation of the flow cytometry γ-H2AX assay. Methods: Here, we report development of a mix-control sample containing peripheral blood mononuclear cells (PBMC) from 10 control individuals, for normalization of day-to-day variation of the flow cytometry-γ-H2AX assay. Results: We showed that control individuals sampled on different days show an average day-to-day variation (CV) of 34%, which was reduced to 12% after normalization to the control sample. The normalization allowed detection of radiosensitivity of lymphoblastoid cell lines from ataxia telangiectasia patients, sampled over three days. Conclusion: The mix-control sample, consisting of 10 control individuals' PBMC, can be used as a control sample to normalize for day-to-day variation of the γ-H2AX assay. The use of this sample will facilitate integration of the γ-H2AX assay into clinical routine. This article is protected by copyright. All rights reserved. Adam Viktorisson, Sherin T Mathew, Ola Hammarsten, Pegah Johansson Wiley-Blackwell |
| shingle_catch_all_3 | A control for the day-to-day normalization of the flow cytometry γ-H2AX assay for clinical routine Background: The phosphorylation of histone H2AX (γ-H2AX) at the DNA double-strand break (DSB) site is frequently used for quantifying DSBs and may be useful as a biomarker for clinical applications. We have previously reported a flow cytometry-based quantification of γ-H2AX for clinical routine. One major challenge, however, is the lack of a control sample for normalization of the day-to-day variation of the flow cytometry γ-H2AX assay. Methods: Here, we report development of a mix-control sample containing peripheral blood mononuclear cells (PBMC) from 10 control individuals, for normalization of day-to-day variation of the flow cytometry-γ-H2AX assay. Results: We showed that control individuals sampled on different days show an average day-to-day variation (CV) of 34%, which was reduced to 12% after normalization to the control sample. The normalization allowed detection of radiosensitivity of lymphoblastoid cell lines from ataxia telangiectasia patients, sampled over three days. Conclusion: The mix-control sample, consisting of 10 control individuals' PBMC, can be used as a control sample to normalize for day-to-day variation of the γ-H2AX assay. The use of this sample will facilitate integration of the γ-H2AX assay into clinical routine. This article is protected by copyright. All rights reserved. Adam Viktorisson, Sherin T Mathew, Ola Hammarsten, Pegah Johansson Wiley-Blackwell |
| shingle_catch_all_4 | A control for the day-to-day normalization of the flow cytometry γ-H2AX assay for clinical routine Background: The phosphorylation of histone H2AX (γ-H2AX) at the DNA double-strand break (DSB) site is frequently used for quantifying DSBs and may be useful as a biomarker for clinical applications. We have previously reported a flow cytometry-based quantification of γ-H2AX for clinical routine. One major challenge, however, is the lack of a control sample for normalization of the day-to-day variation of the flow cytometry γ-H2AX assay. Methods: Here, we report development of a mix-control sample containing peripheral blood mononuclear cells (PBMC) from 10 control individuals, for normalization of day-to-day variation of the flow cytometry-γ-H2AX assay. Results: We showed that control individuals sampled on different days show an average day-to-day variation (CV) of 34%, which was reduced to 12% after normalization to the control sample. The normalization allowed detection of radiosensitivity of lymphoblastoid cell lines from ataxia telangiectasia patients, sampled over three days. Conclusion: The mix-control sample, consisting of 10 control individuals' PBMC, can be used as a control sample to normalize for day-to-day variation of the γ-H2AX assay. The use of this sample will facilitate integration of the γ-H2AX assay into clinical routine. This article is protected by copyright. All rights reserved. Adam Viktorisson, Sherin T Mathew, Ola Hammarsten, Pegah Johansson Wiley-Blackwell |
| shingle_title_1 | A control for the day-to-day normalization of the flow cytometry γ-H2AX assay for clinical routine |
| shingle_title_2 | A control for the day-to-day normalization of the flow cytometry γ-H2AX assay for clinical routine |
| shingle_title_3 | A control for the day-to-day normalization of the flow cytometry γ-H2AX assay for clinical routine |
| shingle_title_4 | A control for the day-to-day normalization of the flow cytometry γ-H2AX assay for clinical routine |
| timestamp | 2025-06-30T23:32:33.240Z |
| titel | A control for the day-to-day normalization of the flow cytometry γ-H2AX assay for clinical routine |
| titel_suche | A control for the day-to-day normalization of the flow cytometry γ-H2AX assay for clinical routine |
| topic | W WW-YZ |
| uid | ipn_articles_6158332 |