A mitogenic lectin from Rhizoctonia bataticola arrests growth, inhibits metastasis and induces apoptosis in human colon epithelial cancer cells
| Publication Date: |
2018-02-01
|
|---|---|
| Publisher: |
Wiley-Blackwell
|
| Electronic ISSN: |
0091-7419
|
| Topics: |
Biology
Chemistry and Pharmacology
Medicine
|
| Published by: |
| _version_ | 1836398770865045504 |
|---|---|
| autor | Prajna Hegde, Sindhura B.R, Bale M Swamy, Shashikala R Inamdar |
| beschreibung | The correlation between colorectal cancer (CRC) progression and altered expression of N -glycans can be considered in search for new biomarkers and anticancer agents to control CRC. Earlier N-glycan specific mitogenic lectin from Rhizoctonia bataticola ( RBL) has been reported which has growth inhibitory and apoptotic effect on human ovarian and leukemic cells, but mitogenic effect on normal PBMCs revealing its clinical potential. Here we report the effect of RBL on human colon cancer HT 29, SW480 and SW620 cell growth and its differential binding to human normal colon and cancer tissues. RBL has strong binding to both primary and metastatic colon cancer cells with MFI of 403, 404 and 192 respectively for HT 29, SW480 and SW620 cells. RBL shows dose and time dependent growth inhibitory effect with IC 50 of 5, 6.4 and 6.8 µg/ml respectively for HT 29, SW480 and SW620 cells. RBL inhibited the clonogenicity of colon carcinoma cells. RBL arrests metastatic SW620 cell growth at S phase, increased hypodiploid population by 6.1, 14.3 and 23.2% respectively at 12, 24 and 36 h. Further, RBL induces SW620 cell apoptosis in time dependent manner, showed increased release of ROS and nuclear degradation compared to lectin untreated control. Adhesion, wound healing, invasion and migration assays demonstrated anti-metastasis effect of RBL in SW620 cells apart from its growth inhibitory effect. Anti angiogenic effect of RBL was demonstrated by CAM assay. All these results show the promising potential of RBL both as diagnostic and therapeutic agent. This article is protected by copyright. All rights reserved |
| citation_standardnr | 6151039 |
| datenlieferant | ipn_articles |
| feed_id | 1831 |
| feed_publisher | Wiley-Blackwell |
| feed_publisher_url | http://www.wiley.com/wiley-blackwell |
| insertion_date | 2018-02-01 |
| journaleissn | 0091-7419 |
| publikationsjahr_anzeige | 2018 |
| publikationsjahr_facette | 2018 |
| publikationsjahr_intervall | 7984:2015-2019 |
| publikationsjahr_sort | 2018 |
| publisher | Wiley-Blackwell |
| quelle | Journal of Cellular Biochemistry |
| relation | http://onlinelibrary.wiley.com/resolve/doi?DOI=10.1002%2Fjcb.26740 |
| search_space | articles |
| shingle_author_1 | Prajna Hegde, Sindhura B.R, Bale M Swamy, Shashikala R Inamdar |
| shingle_author_2 | Prajna Hegde, Sindhura B.R, Bale M Swamy, Shashikala R Inamdar |
| shingle_author_3 | Prajna Hegde, Sindhura B.R, Bale M Swamy, Shashikala R Inamdar |
| shingle_author_4 | Prajna Hegde, Sindhura B.R, Bale M Swamy, Shashikala R Inamdar |
| shingle_catch_all_1 | A mitogenic lectin from Rhizoctonia bataticola arrests growth, inhibits metastasis and induces apoptosis in human colon epithelial cancer cells The correlation between colorectal cancer (CRC) progression and altered expression of N -glycans can be considered in search for new biomarkers and anticancer agents to control CRC. Earlier N-glycan specific mitogenic lectin from Rhizoctonia bataticola ( RBL) has been reported which has growth inhibitory and apoptotic effect on human ovarian and leukemic cells, but mitogenic effect on normal PBMCs revealing its clinical potential. Here we report the effect of RBL on human colon cancer HT 29, SW480 and SW620 cell growth and its differential binding to human normal colon and cancer tissues. RBL has strong binding to both primary and metastatic colon cancer cells with MFI of 403, 404 and 192 respectively for HT 29, SW480 and SW620 cells. RBL shows dose and time dependent growth inhibitory effect with IC 50 of 5, 6.4 and 6.8 µg/ml respectively for HT 29, SW480 and SW620 cells. RBL inhibited the clonogenicity of colon carcinoma cells. RBL arrests metastatic SW620 cell growth at S phase, increased hypodiploid population by 6.1, 14.3 and 23.2% respectively at 12, 24 and 36 h. Further, RBL induces SW620 cell apoptosis in time dependent manner, showed increased release of ROS and nuclear degradation compared to lectin untreated control. Adhesion, wound healing, invasion and migration assays demonstrated anti-metastasis effect of RBL in SW620 cells apart from its growth inhibitory effect. Anti angiogenic effect of RBL was demonstrated by CAM assay. All these results show the promising potential of RBL both as diagnostic and therapeutic agent. This article is protected by copyright. All rights reserved Prajna Hegde, Sindhura B.R, Bale M Swamy, Shashikala R Inamdar Wiley-Blackwell 0091-7419 00917419 |
| shingle_catch_all_2 | A mitogenic lectin from Rhizoctonia bataticola arrests growth, inhibits metastasis and induces apoptosis in human colon epithelial cancer cells The correlation between colorectal cancer (CRC) progression and altered expression of N -glycans can be considered in search for new biomarkers and anticancer agents to control CRC. Earlier N-glycan specific mitogenic lectin from Rhizoctonia bataticola ( RBL) has been reported which has growth inhibitory and apoptotic effect on human ovarian and leukemic cells, but mitogenic effect on normal PBMCs revealing its clinical potential. Here we report the effect of RBL on human colon cancer HT 29, SW480 and SW620 cell growth and its differential binding to human normal colon and cancer tissues. RBL has strong binding to both primary and metastatic colon cancer cells with MFI of 403, 404 and 192 respectively for HT 29, SW480 and SW620 cells. RBL shows dose and time dependent growth inhibitory effect with IC 50 of 5, 6.4 and 6.8 µg/ml respectively for HT 29, SW480 and SW620 cells. RBL inhibited the clonogenicity of colon carcinoma cells. RBL arrests metastatic SW620 cell growth at S phase, increased hypodiploid population by 6.1, 14.3 and 23.2% respectively at 12, 24 and 36 h. Further, RBL induces SW620 cell apoptosis in time dependent manner, showed increased release of ROS and nuclear degradation compared to lectin untreated control. Adhesion, wound healing, invasion and migration assays demonstrated anti-metastasis effect of RBL in SW620 cells apart from its growth inhibitory effect. Anti angiogenic effect of RBL was demonstrated by CAM assay. All these results show the promising potential of RBL both as diagnostic and therapeutic agent. This article is protected by copyright. All rights reserved Prajna Hegde, Sindhura B.R, Bale M Swamy, Shashikala R Inamdar Wiley-Blackwell 0091-7419 00917419 |
| shingle_catch_all_3 | A mitogenic lectin from Rhizoctonia bataticola arrests growth, inhibits metastasis and induces apoptosis in human colon epithelial cancer cells The correlation between colorectal cancer (CRC) progression and altered expression of N -glycans can be considered in search for new biomarkers and anticancer agents to control CRC. Earlier N-glycan specific mitogenic lectin from Rhizoctonia bataticola ( RBL) has been reported which has growth inhibitory and apoptotic effect on human ovarian and leukemic cells, but mitogenic effect on normal PBMCs revealing its clinical potential. Here we report the effect of RBL on human colon cancer HT 29, SW480 and SW620 cell growth and its differential binding to human normal colon and cancer tissues. RBL has strong binding to both primary and metastatic colon cancer cells with MFI of 403, 404 and 192 respectively for HT 29, SW480 and SW620 cells. RBL shows dose and time dependent growth inhibitory effect with IC 50 of 5, 6.4 and 6.8 µg/ml respectively for HT 29, SW480 and SW620 cells. RBL inhibited the clonogenicity of colon carcinoma cells. RBL arrests metastatic SW620 cell growth at S phase, increased hypodiploid population by 6.1, 14.3 and 23.2% respectively at 12, 24 and 36 h. Further, RBL induces SW620 cell apoptosis in time dependent manner, showed increased release of ROS and nuclear degradation compared to lectin untreated control. Adhesion, wound healing, invasion and migration assays demonstrated anti-metastasis effect of RBL in SW620 cells apart from its growth inhibitory effect. Anti angiogenic effect of RBL was demonstrated by CAM assay. All these results show the promising potential of RBL both as diagnostic and therapeutic agent. This article is protected by copyright. All rights reserved Prajna Hegde, Sindhura B.R, Bale M Swamy, Shashikala R Inamdar Wiley-Blackwell 0091-7419 00917419 |
| shingle_catch_all_4 | A mitogenic lectin from Rhizoctonia bataticola arrests growth, inhibits metastasis and induces apoptosis in human colon epithelial cancer cells The correlation between colorectal cancer (CRC) progression and altered expression of N -glycans can be considered in search for new biomarkers and anticancer agents to control CRC. Earlier N-glycan specific mitogenic lectin from Rhizoctonia bataticola ( RBL) has been reported which has growth inhibitory and apoptotic effect on human ovarian and leukemic cells, but mitogenic effect on normal PBMCs revealing its clinical potential. Here we report the effect of RBL on human colon cancer HT 29, SW480 and SW620 cell growth and its differential binding to human normal colon and cancer tissues. RBL has strong binding to both primary and metastatic colon cancer cells with MFI of 403, 404 and 192 respectively for HT 29, SW480 and SW620 cells. RBL shows dose and time dependent growth inhibitory effect with IC 50 of 5, 6.4 and 6.8 µg/ml respectively for HT 29, SW480 and SW620 cells. RBL inhibited the clonogenicity of colon carcinoma cells. RBL arrests metastatic SW620 cell growth at S phase, increased hypodiploid population by 6.1, 14.3 and 23.2% respectively at 12, 24 and 36 h. Further, RBL induces SW620 cell apoptosis in time dependent manner, showed increased release of ROS and nuclear degradation compared to lectin untreated control. Adhesion, wound healing, invasion and migration assays demonstrated anti-metastasis effect of RBL in SW620 cells apart from its growth inhibitory effect. Anti angiogenic effect of RBL was demonstrated by CAM assay. All these results show the promising potential of RBL both as diagnostic and therapeutic agent. This article is protected by copyright. All rights reserved Prajna Hegde, Sindhura B.R, Bale M Swamy, Shashikala R Inamdar Wiley-Blackwell 0091-7419 00917419 |
| shingle_title_1 | A mitogenic lectin from Rhizoctonia bataticola arrests growth, inhibits metastasis and induces apoptosis in human colon epithelial cancer cells |
| shingle_title_2 | A mitogenic lectin from Rhizoctonia bataticola arrests growth, inhibits metastasis and induces apoptosis in human colon epithelial cancer cells |
| shingle_title_3 | A mitogenic lectin from Rhizoctonia bataticola arrests growth, inhibits metastasis and induces apoptosis in human colon epithelial cancer cells |
| shingle_title_4 | A mitogenic lectin from Rhizoctonia bataticola arrests growth, inhibits metastasis and induces apoptosis in human colon epithelial cancer cells |
| timestamp | 2025-06-30T23:32:22.123Z |
| titel | A mitogenic lectin from Rhizoctonia bataticola arrests growth, inhibits metastasis and induces apoptosis in human colon epithelial cancer cells |
| titel_suche | A mitogenic lectin from Rhizoctonia bataticola arrests growth, inhibits metastasis and induces apoptosis in human colon epithelial cancer cells |
| topic | W V WW-YZ |
| uid | ipn_articles_6151039 |