Hyaluronan-binding by CD44 reduces the memory potential of activated murine CD8 T cells
Sally S. M. Lee-Sayer, Nina Maeshima, Meghan N. Dougan, Anita Dahiya, Arif A. Arif, Manisha Dosanjh, Christopher A. Maxwell, Pauline Johnson
Wiley-Blackwell
Published 2018
Wiley-Blackwell
Published 2018
Publication Date: |
2018-01-09
|
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Publisher: |
Wiley-Blackwell
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Print ISSN: |
0014-2980
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Electronic ISSN: |
1521-4141
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Topics: |
Medicine
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Published by: |
_version_ | 1836398740924006400 |
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autor | Sally S. M. Lee-Sayer, Nina Maeshima, Meghan N. Dougan, Anita Dahiya, Arif A. Arif, Manisha Dosanjh, Christopher A. Maxwell, Pauline Johnson |
beschreibung | Expansion and death of effector CD8 T cells are regulated to limit immunopathology and cells that escape contraction go on to generate immunological memory. CD44, a receptor for the extracellular matrix component hyaluronan, is a marker of activated and memory T cells. Here, we show with a murine model that the increase in CD44 expression and hyaluronan binding induced upon CD8 T cell activation was proportional to the strength of TCR engagement, thereby identifying the most strongly activated T cells. When CD44 −/− and CD44 +/+ OT-I CD8 T cells were adoptively transferred into mice challenged with Listeria -OVA, there was a slight increase in the percentage of CD44 +/+ cells at the effector site. However, CD44 +/+ cells were out-competed by CD44 −/− cells after the contraction phase in the lymphoid tissues, and the CD44 −/− cells preferentially formed more memory cells. The hyaluronan-binding CD44 +/+ CD8 effector T cells showed increased pAkt expression, higher glucose uptake, and were more susceptible to cell death during the contraction phase compared to non-binding CD44 +/+ and CD44 −/− OT-I CD8 T cells, suggesting that CD44 and its engagement with hyaluronan skews CD8 T cells towards a terminal effector differentiation state that reduces their ability to form memory cells. This article is protected by copyright. All rights reserved |
citation_standardnr | 6133847 |
datenlieferant | ipn_articles |
feed_id | 2606 |
feed_publisher | Wiley-Blackwell |
feed_publisher_url | http://www.wiley.com/wiley-blackwell |
insertion_date | 2018-01-09 |
journaleissn | 1521-4141 |
journalissn | 0014-2980 |
publikationsjahr_anzeige | 2018 |
publikationsjahr_facette | 2018 |
publikationsjahr_intervall | 7984:2015-2019 |
publikationsjahr_sort | 2018 |
publisher | Wiley-Blackwell |
quelle | European Journal of Immunology |
relation | http://onlinelibrary.wiley.com/resolve/doi?DOI=10.1002%2Feji.201747263 |
search_space | articles |
shingle_author_1 | Sally S. M. Lee-Sayer, Nina Maeshima, Meghan N. Dougan, Anita Dahiya, Arif A. Arif, Manisha Dosanjh, Christopher A. Maxwell, Pauline Johnson |
shingle_author_2 | Sally S. M. Lee-Sayer, Nina Maeshima, Meghan N. Dougan, Anita Dahiya, Arif A. Arif, Manisha Dosanjh, Christopher A. Maxwell, Pauline Johnson |
shingle_author_3 | Sally S. M. Lee-Sayer, Nina Maeshima, Meghan N. Dougan, Anita Dahiya, Arif A. Arif, Manisha Dosanjh, Christopher A. Maxwell, Pauline Johnson |
shingle_author_4 | Sally S. M. Lee-Sayer, Nina Maeshima, Meghan N. Dougan, Anita Dahiya, Arif A. Arif, Manisha Dosanjh, Christopher A. Maxwell, Pauline Johnson |
shingle_catch_all_1 | Hyaluronan-binding by CD44 reduces the memory potential of activated murine CD8 T cells Expansion and death of effector CD8 T cells are regulated to limit immunopathology and cells that escape contraction go on to generate immunological memory. CD44, a receptor for the extracellular matrix component hyaluronan, is a marker of activated and memory T cells. Here, we show with a murine model that the increase in CD44 expression and hyaluronan binding induced upon CD8 T cell activation was proportional to the strength of TCR engagement, thereby identifying the most strongly activated T cells. When CD44 −/− and CD44 +/+ OT-I CD8 T cells were adoptively transferred into mice challenged with Listeria -OVA, there was a slight increase in the percentage of CD44 +/+ cells at the effector site. However, CD44 +/+ cells were out-competed by CD44 −/− cells after the contraction phase in the lymphoid tissues, and the CD44 −/− cells preferentially formed more memory cells. The hyaluronan-binding CD44 +/+ CD8 effector T cells showed increased pAkt expression, higher glucose uptake, and were more susceptible to cell death during the contraction phase compared to non-binding CD44 +/+ and CD44 −/− OT-I CD8 T cells, suggesting that CD44 and its engagement with hyaluronan skews CD8 T cells towards a terminal effector differentiation state that reduces their ability to form memory cells. This article is protected by copyright. All rights reserved Sally S. M. Lee-Sayer, Nina Maeshima, Meghan N. Dougan, Anita Dahiya, Arif A. Arif, Manisha Dosanjh, Christopher A. Maxwell, Pauline Johnson Wiley-Blackwell 0014-2980 00142980 1521-4141 15214141 |
shingle_catch_all_2 | Hyaluronan-binding by CD44 reduces the memory potential of activated murine CD8 T cells Expansion and death of effector CD8 T cells are regulated to limit immunopathology and cells that escape contraction go on to generate immunological memory. CD44, a receptor for the extracellular matrix component hyaluronan, is a marker of activated and memory T cells. Here, we show with a murine model that the increase in CD44 expression and hyaluronan binding induced upon CD8 T cell activation was proportional to the strength of TCR engagement, thereby identifying the most strongly activated T cells. When CD44 −/− and CD44 +/+ OT-I CD8 T cells were adoptively transferred into mice challenged with Listeria -OVA, there was a slight increase in the percentage of CD44 +/+ cells at the effector site. However, CD44 +/+ cells were out-competed by CD44 −/− cells after the contraction phase in the lymphoid tissues, and the CD44 −/− cells preferentially formed more memory cells. The hyaluronan-binding CD44 +/+ CD8 effector T cells showed increased pAkt expression, higher glucose uptake, and were more susceptible to cell death during the contraction phase compared to non-binding CD44 +/+ and CD44 −/− OT-I CD8 T cells, suggesting that CD44 and its engagement with hyaluronan skews CD8 T cells towards a terminal effector differentiation state that reduces their ability to form memory cells. This article is protected by copyright. All rights reserved Sally S. M. Lee-Sayer, Nina Maeshima, Meghan N. Dougan, Anita Dahiya, Arif A. Arif, Manisha Dosanjh, Christopher A. Maxwell, Pauline Johnson Wiley-Blackwell 0014-2980 00142980 1521-4141 15214141 |
shingle_catch_all_3 | Hyaluronan-binding by CD44 reduces the memory potential of activated murine CD8 T cells Expansion and death of effector CD8 T cells are regulated to limit immunopathology and cells that escape contraction go on to generate immunological memory. CD44, a receptor for the extracellular matrix component hyaluronan, is a marker of activated and memory T cells. Here, we show with a murine model that the increase in CD44 expression and hyaluronan binding induced upon CD8 T cell activation was proportional to the strength of TCR engagement, thereby identifying the most strongly activated T cells. When CD44 −/− and CD44 +/+ OT-I CD8 T cells were adoptively transferred into mice challenged with Listeria -OVA, there was a slight increase in the percentage of CD44 +/+ cells at the effector site. However, CD44 +/+ cells were out-competed by CD44 −/− cells after the contraction phase in the lymphoid tissues, and the CD44 −/− cells preferentially formed more memory cells. The hyaluronan-binding CD44 +/+ CD8 effector T cells showed increased pAkt expression, higher glucose uptake, and were more susceptible to cell death during the contraction phase compared to non-binding CD44 +/+ and CD44 −/− OT-I CD8 T cells, suggesting that CD44 and its engagement with hyaluronan skews CD8 T cells towards a terminal effector differentiation state that reduces their ability to form memory cells. This article is protected by copyright. All rights reserved Sally S. M. Lee-Sayer, Nina Maeshima, Meghan N. Dougan, Anita Dahiya, Arif A. Arif, Manisha Dosanjh, Christopher A. Maxwell, Pauline Johnson Wiley-Blackwell 0014-2980 00142980 1521-4141 15214141 |
shingle_catch_all_4 | Hyaluronan-binding by CD44 reduces the memory potential of activated murine CD8 T cells Expansion and death of effector CD8 T cells are regulated to limit immunopathology and cells that escape contraction go on to generate immunological memory. CD44, a receptor for the extracellular matrix component hyaluronan, is a marker of activated and memory T cells. Here, we show with a murine model that the increase in CD44 expression and hyaluronan binding induced upon CD8 T cell activation was proportional to the strength of TCR engagement, thereby identifying the most strongly activated T cells. When CD44 −/− and CD44 +/+ OT-I CD8 T cells were adoptively transferred into mice challenged with Listeria -OVA, there was a slight increase in the percentage of CD44 +/+ cells at the effector site. However, CD44 +/+ cells were out-competed by CD44 −/− cells after the contraction phase in the lymphoid tissues, and the CD44 −/− cells preferentially formed more memory cells. The hyaluronan-binding CD44 +/+ CD8 effector T cells showed increased pAkt expression, higher glucose uptake, and were more susceptible to cell death during the contraction phase compared to non-binding CD44 +/+ and CD44 −/− OT-I CD8 T cells, suggesting that CD44 and its engagement with hyaluronan skews CD8 T cells towards a terminal effector differentiation state that reduces their ability to form memory cells. This article is protected by copyright. All rights reserved Sally S. M. Lee-Sayer, Nina Maeshima, Meghan N. Dougan, Anita Dahiya, Arif A. Arif, Manisha Dosanjh, Christopher A. Maxwell, Pauline Johnson Wiley-Blackwell 0014-2980 00142980 1521-4141 15214141 |
shingle_title_1 | Hyaluronan-binding by CD44 reduces the memory potential of activated murine CD8 T cells |
shingle_title_2 | Hyaluronan-binding by CD44 reduces the memory potential of activated murine CD8 T cells |
shingle_title_3 | Hyaluronan-binding by CD44 reduces the memory potential of activated murine CD8 T cells |
shingle_title_4 | Hyaluronan-binding by CD44 reduces the memory potential of activated murine CD8 T cells |
timestamp | 2025-06-30T23:31:53.781Z |
titel | Hyaluronan-binding by CD44 reduces the memory potential of activated murine CD8 T cells |
titel_suche | Hyaluronan-binding by CD44 reduces the memory potential of activated murine CD8 T cells |
topic | WW-YZ |
uid | ipn_articles_6133847 |