Hyaluronan-binding by CD44 reduces the memory potential of activated murine CD8 T cells

Publication Date:
2018-01-09
Publisher:
Wiley-Blackwell
Print ISSN:
0014-2980
Electronic ISSN:
1521-4141
Topics:
Medicine
Published by:
http://www.wiley.com/wiley-blackwell
_version_ 1836398740924006400
autor Sally S. M. Lee-Sayer, Nina Maeshima, Meghan N. Dougan, Anita Dahiya, Arif A. Arif, Manisha Dosanjh, Christopher A. Maxwell, Pauline Johnson
beschreibung Expansion and death of effector CD8 T cells are regulated to limit immunopathology and cells that escape contraction go on to generate immunological memory. CD44, a receptor for the extracellular matrix component hyaluronan, is a marker of activated and memory T cells. Here, we show with a murine model that the increase in CD44 expression and hyaluronan binding induced upon CD8 T cell activation was proportional to the strength of TCR engagement, thereby identifying the most strongly activated T cells. When CD44 −/− and CD44 +/+ OT-I CD8 T cells were adoptively transferred into mice challenged with Listeria -OVA, there was a slight increase in the percentage of CD44 +/+ cells at the effector site. However, CD44 +/+ cells were out-competed by CD44 −/− cells after the contraction phase in the lymphoid tissues, and the CD44 −/− cells preferentially formed more memory cells. The hyaluronan-binding CD44 +/+ CD8 effector T cells showed increased pAkt expression, higher glucose uptake, and were more susceptible to cell death during the contraction phase compared to non-binding CD44 +/+ and CD44 −/− OT-I CD8 T cells, suggesting that CD44 and its engagement with hyaluronan skews CD8 T cells towards a terminal effector differentiation state that reduces their ability to form memory cells. This article is protected by copyright. All rights reserved
citation_standardnr 6133847
datenlieferant ipn_articles
feed_id 2606
feed_publisher Wiley-Blackwell
feed_publisher_url http://www.wiley.com/wiley-blackwell
insertion_date 2018-01-09
journaleissn 1521-4141
journalissn 0014-2980
publikationsjahr_anzeige 2018
publikationsjahr_facette 2018
publikationsjahr_intervall 7984:2015-2019
publikationsjahr_sort 2018
publisher Wiley-Blackwell
quelle European Journal of Immunology
relation http://onlinelibrary.wiley.com/resolve/doi?DOI=10.1002%2Feji.201747263
search_space articles
shingle_author_1 Sally S. M. Lee-Sayer, Nina Maeshima, Meghan N. Dougan, Anita Dahiya, Arif A. Arif, Manisha Dosanjh, Christopher A. Maxwell, Pauline Johnson
shingle_author_2 Sally S. M. Lee-Sayer, Nina Maeshima, Meghan N. Dougan, Anita Dahiya, Arif A. Arif, Manisha Dosanjh, Christopher A. Maxwell, Pauline Johnson
shingle_author_3 Sally S. M. Lee-Sayer, Nina Maeshima, Meghan N. Dougan, Anita Dahiya, Arif A. Arif, Manisha Dosanjh, Christopher A. Maxwell, Pauline Johnson
shingle_author_4 Sally S. M. Lee-Sayer, Nina Maeshima, Meghan N. Dougan, Anita Dahiya, Arif A. Arif, Manisha Dosanjh, Christopher A. Maxwell, Pauline Johnson
shingle_catch_all_1 Hyaluronan-binding by CD44 reduces the memory potential of activated murine CD8 T cells
Expansion and death of effector CD8 T cells are regulated to limit immunopathology and cells that escape contraction go on to generate immunological memory. CD44, a receptor for the extracellular matrix component hyaluronan, is a marker of activated and memory T cells. Here, we show with a murine model that the increase in CD44 expression and hyaluronan binding induced upon CD8 T cell activation was proportional to the strength of TCR engagement, thereby identifying the most strongly activated T cells. When CD44 −/− and CD44 +/+ OT-I CD8 T cells were adoptively transferred into mice challenged with Listeria -OVA, there was a slight increase in the percentage of CD44 +/+ cells at the effector site. However, CD44 +/+ cells were out-competed by CD44 −/− cells after the contraction phase in the lymphoid tissues, and the CD44 −/− cells preferentially formed more memory cells. The hyaluronan-binding CD44 +/+ CD8 effector T cells showed increased pAkt expression, higher glucose uptake, and were more susceptible to cell death during the contraction phase compared to non-binding CD44 +/+ and CD44 −/− OT-I CD8 T cells, suggesting that CD44 and its engagement with hyaluronan skews CD8 T cells towards a terminal effector differentiation state that reduces their ability to form memory cells. This article is protected by copyright. All rights reserved
Sally S. M. Lee-Sayer, Nina Maeshima, Meghan N. Dougan, Anita Dahiya, Arif A. Arif, Manisha Dosanjh, Christopher A. Maxwell, Pauline Johnson
Wiley-Blackwell
0014-2980
00142980
1521-4141
15214141
shingle_catch_all_2 Hyaluronan-binding by CD44 reduces the memory potential of activated murine CD8 T cells
Expansion and death of effector CD8 T cells are regulated to limit immunopathology and cells that escape contraction go on to generate immunological memory. CD44, a receptor for the extracellular matrix component hyaluronan, is a marker of activated and memory T cells. Here, we show with a murine model that the increase in CD44 expression and hyaluronan binding induced upon CD8 T cell activation was proportional to the strength of TCR engagement, thereby identifying the most strongly activated T cells. When CD44 −/− and CD44 +/+ OT-I CD8 T cells were adoptively transferred into mice challenged with Listeria -OVA, there was a slight increase in the percentage of CD44 +/+ cells at the effector site. However, CD44 +/+ cells were out-competed by CD44 −/− cells after the contraction phase in the lymphoid tissues, and the CD44 −/− cells preferentially formed more memory cells. The hyaluronan-binding CD44 +/+ CD8 effector T cells showed increased pAkt expression, higher glucose uptake, and were more susceptible to cell death during the contraction phase compared to non-binding CD44 +/+ and CD44 −/− OT-I CD8 T cells, suggesting that CD44 and its engagement with hyaluronan skews CD8 T cells towards a terminal effector differentiation state that reduces their ability to form memory cells. This article is protected by copyright. All rights reserved
Sally S. M. Lee-Sayer, Nina Maeshima, Meghan N. Dougan, Anita Dahiya, Arif A. Arif, Manisha Dosanjh, Christopher A. Maxwell, Pauline Johnson
Wiley-Blackwell
0014-2980
00142980
1521-4141
15214141
shingle_catch_all_3 Hyaluronan-binding by CD44 reduces the memory potential of activated murine CD8 T cells
Expansion and death of effector CD8 T cells are regulated to limit immunopathology and cells that escape contraction go on to generate immunological memory. CD44, a receptor for the extracellular matrix component hyaluronan, is a marker of activated and memory T cells. Here, we show with a murine model that the increase in CD44 expression and hyaluronan binding induced upon CD8 T cell activation was proportional to the strength of TCR engagement, thereby identifying the most strongly activated T cells. When CD44 −/− and CD44 +/+ OT-I CD8 T cells were adoptively transferred into mice challenged with Listeria -OVA, there was a slight increase in the percentage of CD44 +/+ cells at the effector site. However, CD44 +/+ cells were out-competed by CD44 −/− cells after the contraction phase in the lymphoid tissues, and the CD44 −/− cells preferentially formed more memory cells. The hyaluronan-binding CD44 +/+ CD8 effector T cells showed increased pAkt expression, higher glucose uptake, and were more susceptible to cell death during the contraction phase compared to non-binding CD44 +/+ and CD44 −/− OT-I CD8 T cells, suggesting that CD44 and its engagement with hyaluronan skews CD8 T cells towards a terminal effector differentiation state that reduces their ability to form memory cells. This article is protected by copyright. All rights reserved
Sally S. M. Lee-Sayer, Nina Maeshima, Meghan N. Dougan, Anita Dahiya, Arif A. Arif, Manisha Dosanjh, Christopher A. Maxwell, Pauline Johnson
Wiley-Blackwell
0014-2980
00142980
1521-4141
15214141
shingle_catch_all_4 Hyaluronan-binding by CD44 reduces the memory potential of activated murine CD8 T cells
Expansion and death of effector CD8 T cells are regulated to limit immunopathology and cells that escape contraction go on to generate immunological memory. CD44, a receptor for the extracellular matrix component hyaluronan, is a marker of activated and memory T cells. Here, we show with a murine model that the increase in CD44 expression and hyaluronan binding induced upon CD8 T cell activation was proportional to the strength of TCR engagement, thereby identifying the most strongly activated T cells. When CD44 −/− and CD44 +/+ OT-I CD8 T cells were adoptively transferred into mice challenged with Listeria -OVA, there was a slight increase in the percentage of CD44 +/+ cells at the effector site. However, CD44 +/+ cells were out-competed by CD44 −/− cells after the contraction phase in the lymphoid tissues, and the CD44 −/− cells preferentially formed more memory cells. The hyaluronan-binding CD44 +/+ CD8 effector T cells showed increased pAkt expression, higher glucose uptake, and were more susceptible to cell death during the contraction phase compared to non-binding CD44 +/+ and CD44 −/− OT-I CD8 T cells, suggesting that CD44 and its engagement with hyaluronan skews CD8 T cells towards a terminal effector differentiation state that reduces their ability to form memory cells. This article is protected by copyright. All rights reserved
Sally S. M. Lee-Sayer, Nina Maeshima, Meghan N. Dougan, Anita Dahiya, Arif A. Arif, Manisha Dosanjh, Christopher A. Maxwell, Pauline Johnson
Wiley-Blackwell
0014-2980
00142980
1521-4141
15214141
shingle_title_1 Hyaluronan-binding by CD44 reduces the memory potential of activated murine CD8 T cells
shingle_title_2 Hyaluronan-binding by CD44 reduces the memory potential of activated murine CD8 T cells
shingle_title_3 Hyaluronan-binding by CD44 reduces the memory potential of activated murine CD8 T cells
shingle_title_4 Hyaluronan-binding by CD44 reduces the memory potential of activated murine CD8 T cells
timestamp 2025-06-30T23:31:53.781Z
titel Hyaluronan-binding by CD44 reduces the memory potential of activated murine CD8 T cells
titel_suche Hyaluronan-binding by CD44 reduces the memory potential of activated murine CD8 T cells
topic WW-YZ
uid ipn_articles_6133847